A threonine synthase homolog from a mammalian genome

The genomes of several vertebrates contain two genes encoding proteins highly similar to threonine synthase (TS), even though the biosynthesis Of L-threonine (L-Thr) is not known to occur in these animals. We report a bioinformatic analysis of the two TS-like genes, the recombinant expression of one murine TS homolog (mTSH2) and its initial biochemical characterization. Recombinant mTSH2 contained bound pyridoxal-5'-phosphate (PLP), but did not synthesize L-Thr. The enzyme did, however, bind O-phospho-homoserine (PHS; the actual TS substrate) and degraded it to alpha-ketobutyrate, phosphate, and ammonia-a known side reaction of microbial TSs. mTSH2 also degraded O-phospho-threonine (PThr) to alpha-ketobutyrate, showing that it can act as a catabolic phospho-lyase on both gamma- and P-phosphorylated substrates. These findings suggest an unusual evolutionary origin for mTSH2, whereby an original TS enzyme became 'recycled' into a phospho-lyase upon dismissal, in metazoa, of the L-Thr biosynthetic pathway

Note Illustrative della Carta geologica d'Italia alla scala 1:50.000, F. 118 Milano, Servizio Geologico d'Italia - ISPRA

Note illustrative redatte per il Foglio geologico n.118 Milano della Carta Geologica d'Italia alla scala 1:50.000. 224 pp

Il progetto HELI-DEM (Helvetia-Italy Digital Elevation Model): scopi e stato di attuazione

The HELI-DEM(Helvetia Italy Digital Elevation Model) project aims to create a unified digital model of heights for the alpine and subalpine zones along the border between Italy and Swizzerland. This model should be properly geo-referenced and produced combining in a single model all the available information. The project is divided into nine Work Packages (WPs), for a total duration of 30 months. The project is now in the ninth month. This paper discusses the purposes of HELI-DEM, presents the different WPs and the present state of the project

Il progetto HELI-DEM (Helvetia-Italy Digital Elevation Model): scopi e stato di attuazione

The HELI-DEM(Helvetia Italy Digital Elevation Model) project aims to create a unified digital model of heights for the alpine and subalpine zones along the border between Italy and Swizzerland. This model should be properly geo-referenced and produced combining in a single model all the available information. The project is divided into nine Work Packages (WPs), for a total duration of 30 months. The project is now in the ninth month. This paper discusses the purposes of HELI-DEM, presents the different WPs and the present state of the projec