Very Rare Complementation between Mitochondria Carrying Different Mitochondrial DNA Mutations Points to Intrinsic Genetic Autonomy of the Organelles in Cultured Human Cells

In the present work, a large scale investigation was done regarding the capacity of cultured human cell lines (carrying in homoplasmic form either the mitochondrial tRNALys A8344G mutation associated with the myoclonic epilepsy and ragged red fiber (MERRF) encephalomyopathy or a frameshift mutation, isolated in vitro, in the gene for the ND4 subunit of NADH dehydrogenase) to undergo transcomplementation of their recessive mitochondrial DNA (mtDNA) mutations after cell fusion. The presence of appropriate nuclear drug resistance markers in the two cell lines allowed measurements of the frequency of cell fusion in glucose-containing medium, non-selective for respiratory capacity, whereas the frequency of transcomplementation of the two mtDNA mutations was determined by growing the same cell fusion mixture in galactose-containing medium, selective for respiratory competence. Transcomplementation of the two mutations was revealed by the re-establishment of normal mitochondrial protein synthesis and respiratory activity and by the relative rates synthesis of two isoforms of the ND3 subunit of NADH dehydrogenase. The results of several experiments showed a cell fusion frequency between 1.4 and 3.4% and an absolute transcomplementation frequency that varied between 1.2 × 10^-5 and 5.5 × 10^-4. Thus, only 0.3-1.6% of the fusion products exhibited transcomplementation of the two mutations. These rare transcomplementing clones were very sluggish in developing, grew very slowly thereafter, and showed a substantial rate of cell death (22-28%). The present results strongly support the conclusion that the capacity of mitochondria to fuse and mix their contents is not a general intrinsic property of these organelles in mammalian cells, although it may become activated in some developmental or physiological situations

Can rigidly rotating polytropes be sources of the Kerr metric?

We use a recent result by Cabezas et al. to build up an approximate solution to the gravitational field created by a rigidly rotating polytrope. We solve the linearized Einstein equations inside and outside the surface of zero pressure including second-order corrections due to rotational motion to get an asymptotically flat metric in a global harmonic coordinate system. We prove that if the metric and their first derivatives are continuous on the matching surface up to this order of approximation, the multipole moments of this metric cannot be fitted to those of the Kerr metric.Comment: LaTeX, 17 pages, submitted to CQ

Cálculo de tensiones en la superficie generalizada de recipientes a presión de composites: criterios de fallo

IX CONGRESO NACIONAL DE MATERIALES COMPUESTOS. Celebrado en Girona los días 5, 6, 7 y 8 de julio de 2011Se revisan las alternativas usadas, en el ámbito de los composites, para la determinación de las tensiones en puntos de la superficie generalizada de recipientes a presión de forma cilíndrica sometidos en exclusiva a la acción de sobrepresión interior. La revisión se complementa con la de los criterios de fallo empleados. Se comparan los resultados obtenidos para el campo de tensiones mediante: modelos analíticos simplificados (Teoría Clásica de Laminados y Cálculo de red), modelos analíticos generales (Formulación de Parnas-Lekhmitskii), modelos numéricos de softwares comerciales y medidas de laboratorio. La comparación de los criterios de fallo implica a los siguientes: Tsai-Hill, Tsai-Wu, Hoffman, Tensión máxima, Deformación máxima y AMS3 (AE). Se señalan los resultados consistentes entre todos los estudios, (ángulo óptimo de tejido y secuencias de apilado) y se evidencian y discuten aquéllos otros contradictorios, dando pie a nuevas líneas de trabaj

Hydrologic and landscape changes in the Middle Ebro River (NE Spain): implications for restoration and management

The changes of landscape (1927–2003), discharge regime and anthropic activities with the river-floodplain of one reach at the Middle Ebro River (NE Spain) were investigated with the objective to identify the factors that best explain the natural ecotope succession and propose a realistic restoration option with consideration of the landscape dynamics during the last century and the socio-economic context. Our results indicate that hydrological and landscape patterns have been dramatically changed during the last century as a consequence of human alteration of the fluvial dynamics within the studied reach. The magnitude and variability of river discharge events have decreased at the end of the last century, and flood protection structures have disrupted the river floodplain connectivity. As a result, the succesional pathways of riparian ecotopes have been heavily modified because natural rejuvenation no longer takes place, resulting in decreased landscape diversity. It is apparent from these data that floodplain restoration must be incorporated as a significant factor into river management plans if a more natural functioning wants to be retrieved. The ecotope structure and dynamics of the 1927–1957 period should be adopted as the guiding image, whereas current hydrologic and landscape (dykes, raised surfaces) patterns should be considered. Under the current socio-economic context, the more realistic option seems to create a dynamic river corridor reallocating dykes and lowering floodplain heights. The extent of this river corridor should adapt to the restored flow regime, although periodic economic investments could be an option if the desired self-sustained dynamism is not reached

Behind taxonomic variability: The functional redundancy in the tick microbiome

The taxonomic composition and diversity of tick midgut microbiota have been extensively studied in different species of the genera Rhipicephalus, Ixodes, Amblyomma, Haemaphysalis, Hyalomma, Dermacentor, Argas and Ornithodoros, while the functional significance of bacterial diversity has been proportionally less explored. In this study, we used previously published 16S amplicon sequence data sets from three Ixodes scapularis cohorts, two of uninfected nymphs, and one of larvae experimentally infected with Borrelia burgdorferi, to test the functional redundancy of the tick microbiome. We predicted the metabolic profiling of each sample using the state-of-the-art metagenomics tool PICRUSt2. The results showed that the microbiomes of all I. scapularis samples share only 80 taxa (24.6%, total 324), while out of the 342 metabolic pathways predicted, 82.7%, were shared by all the ticks. Borrelia-infected larvae lack 15.4% of pathways found in the microbiome of uninfected nymphs. Taxa contribution analysis showed that the functional microbiome of uninfected ticks was highly redundant, with, in some cases, up to 198 bacterial taxa contributing to a single pathway. However, Borrelia-infected larvae had a smaller redundancy with 6.7% of pathways provided by more than 100 genera, while 15.7–19.2% of pathways were provided by more than 100 genera in the two cohorts of uninfected ticks. In addition, we compared the functional profiles of three microbial communities from each data set, identified through a network-based approach, and we observed functional similarity between them. Based on the functional redundancy and functional similarity of the microbiome of ticks in different developmental stages and infection status, we concluded that the tick gut microbiota is a self-regulating community of very diverse bacteria contributing to a defined set of metabolic pathways and functions with yet unexplored relevance for tick fitness and/or bacterial community stability. We propose a change of focus in which the tick microbiome must be analyzed in all dimensions, highlighting their functional traits, instead of the conventional taxonomic profiling

Intrinsic Optical and Electronic Properties from Quantitative Analysis of Plasmonic Semiconductor Nanocrystal Ensemble Optical Extinction

The optical extinction spectra arising from localized surface plasmon resonance in doped semiconductor nanocrystals (NCs) have intensities and lineshapes determined by free charge carrier concentrations and the various mechanisms for damping the oscillation of those free carriers. However, these intrinsic properties are convoluted by heterogeneous broadening when measuring spectra of ensembles. We reveal that the traditional Drude approximation is not equipped to fit spectra from a heterogeneous ensemble of doped semiconductor NCs and produces fit results that violate Mie scattering theory. The heterogeneous ensemble Drude approximation (HEDA) model rectifies this issue by accounting for ensemble heterogeneity and near-surface depletion. The HEDA model is applied to tin-doped indium oxide NCs for a range of sizes and doping levels but we expect it can be employed for any isotropic plasmonic particles in the quasistatic regime. It captures individual NC optical properties and their contributions to the ensemble spectra thereby enabling the analysis of intrinsic NC properties from an ensemble measurement. Quality factors for the average NC in each ensemble are quantified and found to be notably higher than those of the ensemble. Carrier mobility and conductivity derived from HEDA fits matches that measured in the bulk thin film literature