A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world

Archivos de Medicina Veterinaria Cholesterol and vitamin E determination in broiler chickens fed canola oil Cholesterol and vitamin E determination in broiler chickens fed canola oil Determinación de colesterol y vitamina E en pollos broiler alimentados c

RESUMEN Este estudio describe el efecto del aceite de canola sobre las proporciones de colesterol y vitamina E (α and γ tocopherol) en piernas y pechugas de pollos broiler. Ciento veinte pollos broiler de 1 d de edad fueron aleatoriamente asignados dentro de cuatro grupos de 32 pollos cada uno. Cada grupo fue dividido en cuatro subgrupos de ocho pollitos, los que recibieron una de las cuatro dietas conteniendo: 15% de aceite con diferentes porcentajes de aceite de canola (dieta 1: 0%; 2: 5%; 3: 10%; y 4: 15%) por 31 días. Los animales fueron sacrificados al día 45 para la obtención de muestras. Colesterol en piernas y γ tocoferol en piernas y también en pechugas no mostraron una tendencia clara acorde incrementaba el contenido de aceite de canola y decrecía el de aceite de maíz en las dietas (a pesar de las mayores proporciones de γ tocoferol encontradas en el aceite de maíz). En conclusión las proporciones de vitamin E encontradas en el aceite de canola no influenciaron las proporciones de vitamina E en el tejido muscular en pollos broiler. Palabras clave: antioxidantes: α tocoferol, γ tocoferol, carne de pollo. SUMMARY This article describes the effect of canola oil on cholesterol and vitamin E (α and γ tocopherol) proportion in broiler chicken legs and breast. One hundred and twenty eight 1-day old broiler chickens w e r e randomly assigned into 4 groups of 32 chicks each. Each group was divided into 4 subgroups of 8 chicks which received one of the four d i e t s c o n t a i n i n g 15% oil with different percentages of canola oil (diet 1: 0%; 2: 5%; 3: 10%; and 4: 15%) for 31 days. Animals were sacrificed at day 45 to obtain samples. Cholesterol in legs and γ tocopherol in legs and also chicken breast did not show a clear trend according increased canola and decreased corn oil in the diets (despite of the higher γ tocopherol proportions found in corn oil). In conclusion vitamin E proportions found in canola oil did not influence vitamin E proportions in muscle tissue in broiler chickens

A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world