Military Tobacco Dependence Treatment: Implications of Treatment Type on Abstinence and Weight Management Ability

Military readiness, employer productivity and individual health are all significantly impacted by high rates of tobacco use. Current research, to date, suggests that use of brief, individually tailored behavioral modification strategies through group therapy may be most effective in smoking cessation. The objective of this Practice Inquiry Project was to explore aspects of tobacco use the U.S. military population and examine potential relationships between type of treatment, smoking abstinence, and maintenance of body weight (e.g. avoid weight gain) among those seeking tobacco use cessation. The findings of this project can provide guidance on enhancing current tobacco cessation efforts to enable long-term tobacco use abstinence while maintaining body weight among U.S. Navy & Marine (as well as other U.S. military) personnel

A two-step method for identifying photopigment opsin and rhodopsin gene sequences underlying human color vision phenotypes.

PurposeTo present a detailed, reliable long range-PCR and sequencing (LR-PCR-Seq) procedure to identify human opsin gene sequences for variations in the long wavelength-sensitive (OPN1LW), medium wavelength-sensitive (OPN1MW), short wavelength-sensitive (OPN1SW), and rhodopsin (RHO) genes.MethodsColor vision was assessed for nine subjects using the Farnsworth-Munsell 100 hue test, Ishihara pseudoisochromatic plates, and the Rabin cone-contrast threshold procedure (ColorDX, Konan Medical). The color vision phenotypes were normal trichromacy (n = 3), potential tetrachromacy (n = 3), dichromacy (n = 2), and unexplained low color vision (n = 1). DNA was isolated from blood or saliva and LR-PCR amplified into individual products: OPN1LW (4,045 bp), OPN1MW (4,045 bp), OPN1SW (3,326 bp), and RHO (6,715 bp). Each product was sequenced using specific internal primer sets. Analysis was performed with Mutation Surveyor software.ResultsThe LR-PCR-Seq technique identified known single nucleotide polymorphisms (SNPs) in OPN1LW and OPN1MW gene codons (180, 230, 233, 277, and 285), as well as those for lesser studied codons (174, 178, 236, 274, 279, 298 and 309) in the OPN1LW and OPN1MW genes. Additionally, six SNP variants in the OPN1MW and OPN1LW genes not previously reported in the NCBI dbSNP database were identified. An unreported poly-T region within intron 5(c.36+126) of the rhodopsin gene was also found, and analysis showed it to be highly conserved in mammalian species.ConclusionsThis LR-PCR-Seq procedure (single PCR reaction per gene followed by sequencing) can identify exonic and intronic SNP variants in OPN1LW, OPN1MW, OPN1SW, and rhodopsin genes. There is no need for restriction enzyme digestion or multiple PCR steps that can introduce errors. Future studies will combine the LR-PCR-Seq with perceptual behavior measures, allowing for accurate correlations between opsin genotypes, retinal photopigment phenotypes, and color perception behaviors

Partial-Wave Analysis of Single-Pion Production Reactions

We present an overview of our efforts to analyze pion-nucleon elastic scattering data, along with data from related photo- and electroproduction reactions, in order to study the baryon spectrum. We then focus on the Delta(1232) resonance. Fits to pion photo- and electroproduction data have been used to extract values for the R_EM = E2/M1 and R_SM = S2/M1 ratios as functions of Q^2. These results are compared to other recent determinations.Comment: 7 pages, 9 figures, the Shape of Hadrons Workshop Proceedings (27-29 April, 2006 Athens, Greece

Results from the Analysis of Crystal Ball Meson Production Measurements at BNL

The Crystal Ball spectrometer, with its nearly complete angular coverage, is an efficient detector of photon and neutron final states. While installed in the C6 beamline of the Alternating Gradient Synchrotron (AGS) of Brookhaven National Laboratory (BNL), this feature was used in a series of precise measurements of reactions with all-neutral final states. Here we concentrate on the analysis of data from the pion-induced reactions: pi- p --> gamma n, pi- p --> pi0 n, pi- p --> eta n, and pi- p --> pi0 pi0 n.Comment: Conference contribution to MESON 2006 - Krakow, Pola