Salmonella Shedding in Slaughter Pigs and the Use of Esterified Formic Acid in the Drinking Water as a Potential Abattoir-Based Mitigation Measure

Pigs shedding Salmonella at slaughter are considered a source of carcass contamination and human infection. To assess this potential risk, the proportion of Salmonella shedders that arrive for slaughter was evaluated in a population of 1068 pigs from 24 farms. Shedding was present in 27.3% of the pigs, and the monophasic variant of Salmonella Typhimurium, an emerging zoonotic serotype, was the most prevalent (46.9%). Antimicrobial resistance (AMR) in Salmonella isolates was common, but few isolates showed AMR to antimicrobials of critical importance for humans such as third-generation cephalosporins (5%), colistin (0%), or carbapenems (0%). However, AMR to tigecycline was moderately high (15%). The efficacy of an esterified formic acid in the lairage drinking water (3 kg formic acid/1000 L) was also assessed as a potential abattoir-based strategy to reduce Salmonella shedding. It was able to reduce the proportion of shedders (60.7% in the control group (CG) vs. 44.3% in the treatment group (TG); p < 0.01). After considering clustering and confounding factors, the odds of shedding Salmonella in the CG were 2.75 (95% CI = 1.80–4.21) times higher than those of the TG, suggesting a potential efficacy of reduction in shedding as high as 63.6%. This strategy may contribute to mitigating the burden of abattoir environmental contamination

Pengaruh Penambahan Cacahan Limbah Plastik Jenis High Density Polyethylene (Hdpe) Pada

Waste is a very complex problem in urban area. Plastic waste is increasing every year. Kupang with population of 291,794 people generate waste reaches 926 m3/day. Organic waste to 700 m3 and inorganic waste about 226 m3. Concrete is planned by strength quality 25 MPa. Based on the analysis in this study obtained that concrete flexural strength value increased due to the addition of HDPE plastic shredded into the concrete, with chopped levels are added to the concrete at 0%, 0.50% and 0.90% .0,70%. Flexural strength value of normal concrete without the addition of shredded plastic (0%) is 4.12 MPa, flexural strength of concrete with the addition of shredded plastic 0.50% is 4.30 MPa increased 4.37% from normal concrete flexural strength, flexural strength of concrete with the addition of shredded plastics 0.70% is 4.21 MPa increased 2.19% from the normal concrete flexural strength and flexural strength of concrete with the addition of shredded plastic 0.90% is 3.94 MPa decreased 3.64% of flexural strength normal concrete

Salmonella infection in nursery piglets and its role in the spread of salmonellosis to further production periods

Few studies have focused on assessing Salmonella infection in the nursery and its role in further pig production periods. Mesenteric lymph nodes, intestinal content, and meat juice from 389 6-week-old male piglets intended for human consumption from five breeding farms and 191 pooled floor fecal samples from gilt development units (GDU) from the same farms were analyzed to estimate and characterize (by pulsed-field gel electrophoresis and antimicrobial resistance analyses) Salmonella infection. The prevalence of infection and shedding among piglets was 36.5% and 37.3%, respectively, shedding being significantly associated with infection (Odds Ratio = 12.7; CI 7.3–22.0). Salmonella Rissen; S. 4, [5], 12:i:-; and S. Derby were the most common serotypes. A low level of Salmonella-specific maternal antibodies at the beginning of the nursery period suggested it was a period of high risk of infection. Resistance to 3rd-and 4th-generation cephalosporins was detected in piglet isolates although the piglets never received antibiotics, indicating they could be vectors of antimicrobial resistance. The same Salmonella clones were detected in piglet and GDU isolates, suggesting that infected piglets play a significant role in the infection of gilts and consequently of finishing pigs in the case of production farms. The control of Salmonella infection in nursery piglets may decrease the risk of abattoir and carcass contamination

Ocean Wave Energy Converters: Analysis, Modeling, and Simulation. Some case studies

Wave energy has much more potential and benefits than other forms of renewable energy. It is more predictable, consistent, and controllable than wind or solar energy. In this way, an adequate infrastructure can be an alternative and also sustainable system for power supply. In this paper, different wave energy conversion mechanisms (buoys, Pelamis, and oysters) have been described. These models are implemented and simulated using the Design Modeller, ANSYS-AQWA, and WEC-SIM applications. The purpose has been to develop a complete simulation of the wave energy converter and discuss its operation. The analysis has been developed in Matlab-Simulink and both regular and irregular waves have been considered. For this, an approximation to the linear waves theory has been used. The results obtained indicate the energy absorbed from the sea waves and also the energy supplied to the power grid. The simulation results estimated with the different WEC models are comparable to the results shown by other research papers. © 2022, European Association for the Development of Renewable Energy, Environment and Power Quality (EA4EPQ). All rights reserved

Low-cost variable-speed wind turbines design by recycling small electrical machines. Arrangement of permanent magnets in the rotor

This paper describes the design of low-cost variable-speed wind turbines by recycling small electrical machines. In this way, electrical machines such as automobile claw-pole alternators, induction motors for domestic applications, or simply electric motors for some industrial applications are studied, considering their reuse as permanent magnet synchronous generators (PMSG) in small wind turbines or hydro-power turbines. The main purpose is the integration of hybrid energy conversion systems (wind and hydraulic turbines) in small stand-alone microgrids within the rural environment. Likewise, in order to optimize the design, the arrangement of the permanent magnets in the rotor is analyzed. The analysis has been carried out using the FluxMotor simulation software, which is based on the 2D finite element method. At the same time, the FEM software provides a lot of information about the optimization of the electrical machine and its multiple design options and topologies. Suggested designs have similar performance as well as a similar size and weight. The purpose has been to explore different topologies and select the most efficient designs. In this way, it is shown that it is possible to reuse an electrical generator easily, without losing much of the general performance. © 2022, European Association for the Development of Renewable Energy, Environment and Power Quality (EA4EPQ). All rights reserved

Inducible model for β-six-mediated site-specific recombination in mammalian cells

The prokaryotic β recombinase catalyzes site-specific recombination between two directly oriented minimal six sites in chromatin-integrated substrates. Here, we demonstrate that an enhanced green fluorescent protein (EGFP)-fused version of β recombinase (β-EGFP) is fully active, retaining most specific activity. It is used to develop a recombination-dependent activatable gene expression (RAGE) system based on the androgen receptor (AR) ligand-binding domain (LBD). Two hybrid molecules, a direct fusion of the LBD-AR to the C-terminus of β recombinase (β-AR) and a triple fusion of β-EGFP to the same ligand-binding domain (β-EGFP-AR), were engineered and their subcellular behavior, stability and catalytic activity were evaluated. Both chimeric β recombinase proteins showed in vivo inducible recombinogenic activity dependent on addition of an androgen receptor agonist, although the β-AR fusion protein demonstrated more accurate ligand-dependent translocation from cytoplasm to nucleus

Reactive trityl derivatives: stabilised carbocation mass-tags for life sciences applications

The rational design of novel triarylmethyl (trityl)-based mass tags (MT) for mass-spectrometric (MS) applications is described. We propose a "pKR+ rule" to correlate the stability of trityl carbocations with their MS performance: trityls with higher pKR+ values ionise and desorb better. Trityl blocks were synthesised that have high pKR+ values and are stable in conditions of MS analysis; these MTs can be ionised by matrix as well as irradiation with a 337 nm nitrogen laser. 13C-Labelled tags were prepared for MS quantitation applications. Moreover, the tags were equipped with a variety of functional groups allowing conjugation with different functionalities within (bio)molecules to enhance the MS characteristics of the latter. The MS behaviour of model polycationic trityl compounds with and without the matrix was studied to reveal that poly-trityl clusters are always singly charged under the (MA)LDI-TOF conditions. Several peptide-trityl conjugates were prepared and comparisons revealed a beneficial effect of trityl tags on the conjugate detection in MS. Trityl compounds containing para-methoxy- and dimethylamine groups, as well as a xanthene fragment, showed considerable enhancement in MS detection of model peptides; thus they are promising tools for proteomic applications. Dimethoxytrityl derivatives allow one to distinguish between Arg- and Lys-containing peptides. Maleimido trityl derivatives are suitable for the efficient derivatisation of thiol-containing peptides in pyridine

Synthesis and Biological Evaluation of New CRH Analogues

A series of 7 new human/rat Corticotropin Releasing Hormone (h/r-CRH) analogues were synthesized. The induced alterations include substitution of Phe at position 12 with D-Phe, Leu at positions 14 and 15 with Aib and Met at positions 21 and 38 with Cys(Et) and Cys(Pr). The analogues were tested regarding their binding affinity to the CRH-1 receptor and their activity which is represented by means of percentage of maximum response in comparison to the native molecule. The results indicated that the introduction of Aib, or Cys derivatives although altering the secondary structure of the molecule, did not hinder receptor recognition and binding