Effect of different olive oil-derived antioxidants (hydroxytyrosol and 3,4-dihydroxyphenylglycol) on the quality of frozen-thawed ram sperm

The aim of the present study was to evaluate the effect of the addition of different concentrations of two olive oil-derived antioxidants, hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) and 3,4-dihydroxyphenylglycol (DHPG), on ovine semen during the freezing-thawing process. Sperm was collected, pooled and diluted with commercial extenders and then divided into aliquots supplemented with different concentrations (10 μg/ml, 30 μg/ml, 50 μg/ml and 70 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. A control group, without antioxidant, was also prepared. Sperm motility, viability, acrosome integrity, mitochondrial membrane potential and lipid peroxidation (LPO) were assessed. The results showed that frozen-thawed ram spermatozoa exhibited lower values for motility, membrane integrity, acrosome and mitochondrial membrane potential than fresh samples (P ≤ 0.01). However, when antioxidants were added, thawed spermatozoa exhibited relatively low LPO, recording values similar to fresh spermatozoa; by contrast, the control group of frozen-thawed spermatozoa without antioxidants exhibited significantly higher LPO (P ≤ 0.01). The addition of a HT+DHPG mixture (MIX) had a negative impact on sperm membrane and acrosome integrity, suggesting that a pure antioxidant supplementation has the potential to offer superior results. In conclusion, HT and DHPG exhibited a positive effect on the frozen-thawed spermatozoa inasmuch as they reduced the LPO. These olive oil-derived antioxidants have the potential to improve frozen-thawed sperm quality, although further studies should be carried out to analyse the antioxidant effect at different times after thawing.The authors would like to acknowledge Diputacion de Cordoba (Spain) for their help with the supply of semen samples.Peer Reviewe

Anti-Inflammatory local effect of hydroxytyrosol combined with pectin-alginate and olive oil on trinitrobenzene sulfonic acid-induced colitis in wistar rats

Purpose: Evaluate the efficacy of hydroxytyrosol in the local treatment of inflammatory colitis. Currently, the existing treatments for inflammatory bowel diseases does not cure the disease and it is associated with high rates of side effects and complications. Hydroxytyrosol is a phenyl-ethyl-alcohol derived from the hydrolysis of oleuropein and present in olive oil, previous studies have demonstrated the anti-inflammatory effect of dietary hydroxytyrosol supplement, with no toxicity. Materials & Methods: Colitis has been induced by using Trinitrobenzene Sulfonic Acid at 40 rats. They were divided into four groups randomly: 10 rats without treatment; 10 rats with pectin/alginate mixture; 10 rats treated with pectin/alginate + olive oil; 10 rats treated with pectin/alginate + olive oil + hydroxytyrosol. Animals were sacrificed 10 days after induction of trinitrobenzene sulfonic acid, receiving 5 days of continuous treatment. Samples of the rectal area were studied and observed under a microscope to determine the damage by Hunter scoring modified, assessing inflammatory infiltration, number of intestinal walls involved, damage to the mucosal architecture, and edema. Results: When the rectum was analyzed in a global way, nonsignificant differences were observed; however, when performing an individualized analysis, statistically significant differences in the inflammatory infiltrate are present in the samples, which were evaluated using the ANOVA and Student-T statistics. Conclusions: Local treatment with the natural antioxidant hydroxytyrosol combined with pectin/alginate and olive oil of inflammatory bowel disease has been shown to be effective against inflammatory infiltration of TNBS-induced colitis.This research was supported by the Spanish Min-istry of Economy, Industry and Competitivenessand cofunded by a European Social Fund (ESF)(Ramon y Cajal Programme RyC 2012–10456; projectAGL2013–48291-R and project AGL2016-79088-R) andA. Bermúdez-Oria received funding from the SpanishFPI program (MEIC) (BES-2014-068508)Peer Reviewe

Efecto del 3,4-dihidroxifenilglicol en la calidad de semen de gallo (Gallus gallus) criopreservado

6 Páginas.-- 2 tablas[EN]The present study aimed to evaluate the effect of adding 3,4-dihydroxyphenylglycol (DHPG), that is an antioxidant derived from the olive tree (Olea europea), to the cryopreservation extender on post-thaw sperm quality in roosters. For this, 6 replicas of a semen pool which were obtained from 16 Utrerana breed roosters were cryopreserved. From each replicate, four aliquots containing different DHPG concentrations were obtained: Control: no DHPG; T1: DHPG 50 µg/mL; T2: DHPG 100 µg/mL and T3: DHPG 150 µg/mL. It was observed that the values of viability, acrosome integrity, HOST, total motility, progressive motility, linear velocity, rectilinear velocity, curvilinear velocity, and the mean amplitude of lateral displacement of the sperm head decreased significantly (P>0.05) in samples subjected to freezing compared to those of fresh semen. However, for samples subjected to cryopreservation, no significant differences (P<0.05) were observed for these parameters between the control treatment and samples supplemented with different concentrations of DHPG. In conclusion, the addition of these concentrations of DHPG to the cryopreservation extender had no significant effect on the different semen quality parameters evaluated in cryopreserved rooster semen. Thus, future studies increasing the concentration of this antioxidant become necessary.[ES]Efecto del 3,4-dihidroxifenilglicol en la calidad de semen de gallo (Gallus gallus) criopreservado Díaz Ruíz, E.1@; León Jurado, J.M.2; Bermúdez Oria, A.3; González Ariza, A.1,2; Fernández Prior, A.3; Navas González, F.J.1,4; Peláez Caro, M.1,5; Delgado Bermejo, J.V.1 y Arando Arbulu, A.1 1 Departamento de Genética. Universidad de Córdoba. Córdoba. España. 2 Centro Agropecuario Provincial. Diputación de Córdoba. Córdoba. España. 3 Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC). Sevilla. España. 4 Instituto de Investigación y Formación Agraria y Pesquera (IFAPA), Alameda del Obispo. Córdoba. España. 5 Asociación Nacional de Criadores de Caprino de Raza Murciano Granadina. Granada. España. Palabras Clave Antioxidante. Criopreservación. Semen. Avicultura. RESUMEN El objetivo del presente estudio fue evaluar el efecto de la adicción de diferentes concentraciones de 3,4-dihidroxifenilglicol (DHPG), antioxidante derivado del olivo (Olea europea), en el diluyente de crio- preservación sobre la calidad espermática post-descongelación en gallos. Para ello, se crioperservaron 5 réplicas de un pool de semen que procedían de 16 gallos de la raza Utrerana. De cada réplica, se obtuvieron cuatro alícuotas que contenían diferentes concentraciones de DHPG: Control (sin DHPG); T1: DHPG 50 μg/mL; T2: DHPG 100 μg/mL y T3: DHPG 150 μg/mL. Finalmente, las muestras se descon- gelaron y se evaluaron los siguientes parámetros: motilidad espermática, morfología, funcionalidad de membrana (HOST), viabilidad espermática, integridad del acrosoma, glutatión, concentración de EROs y peroxidación lipídica (LPO). Se observó que los valores de viabilidad, integridad del acrosoma, fun- cionalidad de membrana, motilidad total, motilidad progresiva y parámetros cinemáticos disminuyeron significativamente (P>0.05) en las muestras sometidas a congelación en comparación con las del semen fresco. Sin embargo, en cuanto a las muestras sometidas a criopreservación, no se apreciaron diferencias significativas (P<0.05) para estos parámetros entre el tratamiento control y las muestras suplementadas con diferentes concentraciones de DHPG. En conclusión, la adición de estas concentraciones de DHPG al diluyente de criopreservación no tuvo ningún efecto significativo sobre los distintos parámetros de calidad seminal evaluados en semen de gallo criopreservado. Así, futuros estudios aumentando la concentración de este antioxidante se tornan necesariosLos autores quieren agradecer su colaboración al Centro Agropecuario de la Diputación de Córdoba por el mantenimiento de los animales empleados en el presente estudioPeer reviewe

3,4-dihydroxyphenylglycol reduces lipid peroxidation during the cryopreservation process of ram semen

5 Páginas.--1 FiguraThe damage produced by cryopreservation processes on ovine spermatozoa reduces semen quality and therefore its fertilising capacity. The aim of this study was to evaluate the effect of adding different concentrations of 3,4-dihydroxyphenylglycol (DHPG) to sheep semen during the cryopre-servation process. For this purpose, the sperm, once collected, were pooled and diluted with Biladyl, supplemented with different concentrations of DHPG (59 μM, 177μM, 295μM and 413μM), leaving a control treatment without antioxidant supplementation. Motility, viability, acrosome integrity, mito-chondrial membrane potential and lipid peroxidation (LPO) were assessed. No significant differences were observed derived from the addition of DHPG for all the studied variables, with the exception of the LPO, which was reduced in comparison to the control treatment when the samples were cryopre-served. It was therefore concluded that the addition of DHPG has a positive effect on thawed sperm as they reduced LPO.Esta investigación ha sido financiada por el Programa Torres Quevedo del Ministerio de Ciencia e Innovación de España, número de subvención PTQ2019‐010670, concedida a Ander Arando ArbuluPeer reviewe