10 research outputs found
Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae
CARBON NANOTUBES–IONIC LIQUID GEL. CHARACTERIZATION AND APPLICATION TO PSEUDOEPHEDRINE AND CHLORPHENIRAMINE DETERMINATION IN PHARMACEUTICALS
Gold nanoparticles as an efficient drug delivery system for GLP - 1 peptides
In this work, the potential application of gold nanoparticles for GLP-1 analogues delivery was studied. For this purpose, the original sequence of the incretin GLP-1 was slightly modified in the C-terminal region by adding a cysteine residue to facilitate conjugation to the gold surface. The interaction between peptides and gold nanoparticles and also the colloid stability of the conjugates were studied by UV-vis spectrophotometry, TEM, IR and XPS spectroscopy. Moreover, the permeability of these conjugates was assayed using a Caco-2/goblet monolayer model. On the basis of the stability and permeability results, one of the conjugates was chosen to be administered intraperitoneally to normoglycemic rats. The intraperitoneal delivery of the GLP-1 analogue using gold nanoparticles led to decrease levels of blood glucose in the same way as native GLP-1, thereby demonstrating that the formulation of the analogue is stable in physiological conditions and maintains the activity of this incretin. (C) 2017 Elsevier B.V. All rights reserved.CONICYT (Chile)
ATD-24110090
AECID (Spain)
A1/035396/11
CONICYT
Vicerectory of Academic Issues (University of Chile)
Fondap
1513001