Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus-3

<p><b>Copyright information:</b></p><p>Taken from "Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus"</p><p>http://www.biomedcentral.com/1471-213X/7/69</p><p>BMC Developmental Biology 2007;7():69-69.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC1906761.</p><p></p>ve non-specific staining was seen throughout the embryo. B, Thymus-specific expression after removal of the cassette. The heads have been removed from both embryos to allow visualization of the thymic primordia. Arrows indicate one of the thymic lobes. h = heart

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus-4

<p><b>Copyright information:</b></p><p>Taken from "Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus"</p><p>http://www.biomedcentral.com/1471-213X/7/69</p><p>BMC Developmental Biology 2007;7():69-69.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC1906761.</p><p></p>ength product and the 124 bp exon 2–4 spliced product are first seen at 24 cycles in all genotypes. B-E, Keratin 5 and keratin 8 expression in the E16.5 thymus. Transverse sections through E16.5 thymic lobes from (B) wild-type, (C) , (D) and (E) embryos. Keratin 8 is shown in green and marks cortical epithelial cells, while keratin 5 marks medullary epithelial cells and is shown in red. F, FACS analysis of thymocytes from and mice. Thymocytes from newborn mice were stained with anti-CD4-APC, anti-CD8-FITC, anti-CD44-PE, and anti-CD25-Biotin followed by Streptavidin-PerCP. The upper panels show expression of CD4 and CD8 on total thymocytes. The lower panels show expression of CD44 and CD25 on the gated CD4CD8subpopulation

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus-0

<p><b>Copyright information:</b></p><p>Taken from "Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus"</p><p>http://www.biomedcentral.com/1471-213X/7/69</p><p>BMC Developmental Biology 2007;7():69-69.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC1906761.</p><p></p>nking probes (fp) are shown. Exons 7–9 are shown as boxes, coding regions are black and UTR sequences are grey. IRES-Cre or IRES-lacZ cassettes including the floxed or frt neo positive selection cassette were inserted into a unique XhoI site (X) in the 3'UTR in exon 9. The targeting vector homology was flanked by two TKgenes for negative selection. B. Southern blot analysis of ES cell clones. Genomic DNA was digested with EcoRV, and probes with the 5' flanking probe. The wild-type 14.8 kb band is present in all samples. Correctly targeted cell lines with bands corresponding to the two different targeted alleles are present in lanes 3–6. Lane two shows an abberant clone. C. PCR analysis of the lacZ alleles used for genotypeing. The larger, 600 bp band is the wild-type allele, the 300 bp band is the lacZ (z) allele. Marker (M) shown is the Promega 1 kb ladder. V, EcoRV; E, EcoRI; B, BamHI; K, KpnI; S, SspI; X, XhoI

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus-5

<p><b>Copyright information:</b></p><p>Taken from "Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus"</p><p>http://www.biomedcentral.com/1471-213X/7/69</p><p>BMC Developmental Biology 2007;7():69-69.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC1906761.</p><p></p>nking probes (fp) are shown. Exons 7–9 are shown as boxes, coding regions are black and UTR sequences are grey. IRES-Cre or IRES-lacZ cassettes including the floxed or frt neo positive selection cassette were inserted into a unique XhoI site (X) in the 3'UTR in exon 9. The targeting vector homology was flanked by two TKgenes for negative selection. B. Southern blot analysis of ES cell clones. Genomic DNA was digested with EcoRV, and probes with the 5' flanking probe. The wild-type 14.8 kb band is present in all samples. Correctly targeted cell lines with bands corresponding to the two different targeted alleles are present in lanes 3–6. Lane two shows an abberant clone. C. PCR analysis of the lacZ alleles used for genotypeing. The larger, 600 bp band is the wild-type allele, the 300 bp band is the lacZ (z) allele. Marker (M) shown is the Promega 1 kb ladder. V, EcoRV; E, EcoRI; B, BamHI; K, KpnI; S, SspI; X, XhoI

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus-1

<p><b>Copyright information:</b></p><p>Taken from "Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus"</p><p>http://www.biomedcentral.com/1471-213X/7/69</p><p>BMC Developmental Biology 2007;7():69-69.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC1906761.</p><p></p>ression. Black arrows in (A-I) point to the third pharyngeal pouch. White arrows in (J-L) indicate the -negative parathyroid region of the primordium. For sagittal sections, ventral is left and anterior is up. For transverse sections, dorsal is up. A, B, Lateral views of E10.5 (A) and ;R26R(B) whole embryos, and D, E, coronal sections through E10.5 (D) and ;R26R(E) embryos showing no expression in the third pharyngeal pouch. G, H, Lateral views of E11.5 (G) and ;R26R(H) embryos showing expression only in the developing thymus primordium. J, K, Sagittal sections through E11.5 embryo (J) and E11.5 ;R26Rembryo (K) showing expression restricted to the ventral thymus domain of the 3pouch-derived organ primordium. M, Sagittal section through E12.5 thymus primordium. N, Transverse section through E12.5 ;R26Rthymus primordium. O, Transverse section through E12.5 thymus showing hybridization for mRNA. P, Section through E16.5 thymus. Q, R, Sections through E16.5 (Q) and newborn (R) ;R26Rthymi showing more intense staining in the prospective medullary regions. c = cortex, m = medulla

Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus-2

<p><b>Copyright information:</b></p><p>Taken from "Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the locus"</p><p>http://www.biomedcentral.com/1471-213X/7/69</p><p>BMC Developmental Biology 2007;7():69-69.</p><p>Published online 18 Jun 2007</p><p>PMCID:PMC1906761.</p><p></p> Left panel shows the percentage of YFP positive cells in the CD45MHC Class IIpopulation. Right panel shows the percentage of YFP positive cells in the CD45UEA1population