6 research outputs found
(The) effect of gentamicin on calcim transport in normal rabbit lenses according to the route of administration
์ํ๊ณผ/๋ฐ์ฌ[ํ๊ธ]
์๋ด ์์ ์ด๋ ์ฒ๊ณต์ฑ ์์ธ์์ ๋ฐ์ ํ ์ธ๊ท ์ ์๋ด ์นจํฌ๋ก ์ธํ์ฌ ์ด์์ฒด๋ด์ ์ผ์ฆ์ ์ผ์ผํค๋ ์ธ๊ท ์ฑ ์๋ด์ผ์ ์์ผ๋ด์ ์๊ตฌ์ ์น๋ช
์ ์ธ ์์์ ์ค ์ ์๋ค. ์ต๊ทผ์๋ ๋
น๋๊ท ์ ํฌํจํ ๊ทธ๋ ์์ฑ ๊ฐ๊ท ๋ค์ ์ํ ๊ฒฝ์ฐ๊ฐ ์ฆ๊ฐํ๋ ์ถ์ธ๋ก, ์ด๋ฐ ๊ฒฝ์ฐ ๋ณ์ ์งํ์ด
๋น ๋ฅด๊ณ ์ฌํ๋ฉฐ ์ํ๊ฐ ๋๋จํ ๋ถ๋ํ์ฌ ์๊ตฌ๊ฐ ์์ค๋๊ธฐ๋ ํ๋ค. ์ด์ ์น๋ฃ์ ์ ์, ๊ฒฝ๊ตฌํฌ์ฌ, ๊ทผ์ก์ฃผ์ฌ, ์ ๋งฅ์ฃผ์ฌ ๋ฐ ๊ฒฐ๋งํ์ฃผ์ฌ ๋ฑ์ ์ผ๋ฐ์ ์ธ ํญ์์ ํฌ์ฌ ๋ฐฉ๋ฒ์ผ๋ก๋ ํ์ก๋ง๋ง์ฑ
์ด๋ ํ์ก๋ฐฉ์์ฑ
๋ฑ์ ์๊ตฌ์ ํด๋ถ์๋ฆฌํ์ ํน์ง์ผ๋ก ์ธํด ์ด์์ฒด๋ด๋ก ์ฝ๋ฌผ์ ์นจํฌ๊ฐ
์ฝ๊ฒ ์ด๋ฃจ์ด์ง์ง ์์ ์น๋ฃ ํจ๊ณผ๋ฅผ ๊ธฐ๋ํ ์ ์๋ค.
์ธ๊ท ์ฑ ์๋ด์ผ์ ์น๋ฃ์๋ ๊ด๋ฒ์ ํญ์์ ๋ฅผ ์ด์์ฒด๋ด๋ก ์ง์ ์ฃผ์ฌํ๋ ๋ฐฉ๋ฒ์ด ๊ฐ์ฅ ์ ํจํ๊ณ ์ ํฉํ๋ค๊ณ ์๋ ค์ ธ ์๋ค. ํนํ ๋
น๋๊ท ์ ๋ํด ์ ํจํ aminoglycoside ๊ณ์ด์ gentamicin์ ๊ฐ์ฅ ๋๋ฆฌ ์ฌ์ฉ๋๊ณ ์๋ ์์ฉ์ฑ์ ๊ด๋ฒ์ ํญ์๋ฌผ์ง๋ก์ ๊ทธ๋ ์์ฑ๊ท ๊ณผ ์์ฑ๊ท
๋ชจ๋์ ํจ๊ณผ๊ฐ ์๋๋ฐ, gentamicin์ ์ ๋ฐฉ์ด๋ ์ด์์ฒด๋ด๋ก ์ฃผ์ฌํจ์ผ๋ก์จ ์ธ๊ท ์ฑ ์๋ด์ผ์ ๋ํ ์น๋ฃ ํจ๊ณผ๋ฅผ ๊ธฐ๋ํ ์ ์๊ฒ ๋์๋ค.
๊ทธ๋ฌ๋ gentamicin์ ์ด์ ๊ฐ์ ์ฌ๋ฌ ๊ฒฝ๋ก๋ฅผ ํตํด ํฌ์ฌํ๋ ๊ฒฝ์ฐ gentamicin์ด ์ฃผ์ ์๊ตฌ์กฐ์ง์ ์นจํฌํ์ฌ ์ธํฌ๊ธฐ๋ฅ์ ์ด์์ ์ด๋ํ ์ ์์ผ๋ฉฐ, ๊ทธ์ค ์์ ์ฒดํผํ์ด๋ ๋ง๋ง์ ๋
์ฑ์ ์ผ์ผํค๋ ๊ฒฝ์ฐ๊ฐ ๊ฐ์ฅ ๋ฌธ์ ๊ฐ ๋๋ค. ์ด๊ฒ์ gentamicin์ด ์ธํฌ๋ด์ calcium๋๋๋ฅผ
์ฆ๊ฐ์์ผ ๋
์ฑ์ ์ผ์ผํค๊ธฐ ๋๋ฌธ์ธ๋ฐ, ์์ ์ฒด์์๋ calcium transport system์ด ์ต์ ๋์ด ์์ ์ฒด๋ด์ calcium๋๋๊ฐ ์ฆ๊ฐ๋์ด ๋ฐฑ๋ด์ฅ์ด ์ ๋ฐ๋๋ค๊ณ ํ๋ค. ์ต๊ทผ์ ์ฐ๊ตฌ์์๋ ์ ์ถ๋ ์ ์๊ฐํ ์ ์์ ์ฒด๋ฅผ (45)**Ca์ด ํฌํจ๋ ์ฉ์ก๋ด์์ incubation์ํจ ํ gentamicin์
ํฌ์ฌํ ๋ (45)**Ca์ ๋ฅ๋์ ์ ์ถ์ ๊ฐ์์ํค๊ณ gentamicin์ด Ca**++-ATPase์ ํ์ฑ๋๋ฅผ ์ต์ ์ํค๋ฉฐ, Ca**++์ด ์์ ์ฒด๋ด์ ์ถ์ ๋๋ค๋ ๊ฒ์ด ๋ณด๊ณ ๋์๋ค.
์ด์ ์ ์๋ ์ ์๊ฐํ ์์ gentamicin์ ์ ๋ฐฉ ๋ฐ ์ด์์ฒด๋ด๋ก ์ฃผ์ฌํ๊ณ , 1, 3 ๋ฐ 7์ผ ํ์ ์์ ์ฒด๋ฅผ ์ ์ถํ์ฌ ์์ ์ฒด์ Ca**++-activated ATPase ํ์ฑ๋์ ์์ ์ฒด๋ญ์ ํตํ Ca**
++ ์ ์ถ, ์์ ์ฒด๋ด์ Ca**++ ๋๋๋ฅผ ์ธก์ ํ์ฌ, ์ธ๊ท ์ฑ ์๋ด์ผ์ ์น๋ฃ์ gentamicin ํฌ์ฌ ๋ฐฉ๋ฒ์ ๋ฐ๋ฅธ ์์ ์ฒด์์ calcium ์ด๋์ ๋น๊ตํ์ฌ ๋ค์๊ณผ ๊ฐ์ ๊ฒฐ๊ณผ๋ฅผ ์ป์๋ค.
1. ์์ ์ฒด์ Ca**++-ATPase ํ์ฑ๋๋ ์๋ฆฌ์์ผ์ ์ฃผ์ฌ๊ตฐ์์๋ ์ฃผ์ฌํ ์๊ฐ์ด ๊ฒฝ๊ณผํ์ฌ๋ ๋ณํ๊ฐ ์์์ผ๋, gentamicin ์ฃผ์ฌ๊ตฐ์์๋ ์ฃผ์ฌํ ์๊ฐ์ด ๊ฒฝ๊ณผํจ์ ๋ฐ๋ผ ๋์กฐ๊ตฐ์ ๋นํด ์์ ์๊ฒ ์ต์ ๋จ์ ์ ์ ์์๋ค.
2. ์์ ์ฒด์์์ Ca**++ ์ ์ถ์ gentamicin ์ฃผ์ฌ๊ตฐ์ด ์๋ฆฌ์์ผ์ ์ฃผ์ฌ๊ตฐ์ ๋นํด ์์์๊ฒ ๊ฐ์๋์ด ์์์ ์ ์ ์์๋ค.
3. ์์ ์ฒด๋ด์ Ca**++ ๋๋๋ ์๋ฆฌ์์ผ์ ์ฃผ์ฌ๊ตฐ์์๋ ์ฃผ์ฌํ ์๊ฐ์ด ๊ฒฝ๊ณผํ์ฌ๋ ๋ณํ๊ฐ ์์์ผ๋, gentamicin ์ฃผ์ฌ๊ตฐ์์๋ ์ฃผ์ฌํ ์๊ฐ์ด ๊ฒฝ๊ณผํจ์ ๋ฐ๋ผ ์์์๊ฒ ์ฆ๊ฐํจ์ ์ ์ ์์๋ค.
4. ์์ ์ฒด์ Ca**++-ATPase ํ์ฑ๋์ ์ต์ , ์์ ์ฒด์์์ Ca**++ ์ ์ถ์ ๊ฐ์ ๋ฐ ์์ ์ฒด๋ด Ca**++ ๋๋์ ์ฆ๊ฐ๋ gentamicin์ ์ ๋ฐฉ๋ด ์ฃผ์ฌํ ๊ฒฝ์ฐ๋ณด๋ค ์ด์์ฒด๋ด ์ฃผ์ฌํ ๊ฒฝ์ฐ์ ๋ ์ฌํ์๋ค.
์ด์์ ๊ฒฐ๊ณผ๋ก ๋ณด์ gentamicin์ฃผ์ฌ์ ์์ ์ฒด๋ด์ Ca**++ ๋๋์ ์ฆ๊ฐ๋ ์์ ์ฒด Ca**++-ATPase ํ์ฑ๋์ ์ต์ ๋ก ์ธํ ์์ ์ฒด๋ญ์ ํตํ Ca**++ ์ ์ถ์ ๊ฐ์์ ๊ธฐ์ธํ๋ ๊ฒ์ผ๋ก ์๊ฐ๋๋ฉฐ, ์ด๋ฐ ํ์์ ์ด์์ฒด๋ด ์ฃผ์ฌ๊ตฐ์์ ์ ๋ฐฉ๋ด ์ฃผ์ฌ๊ตฐ๋ณด๋ค ๋ ํ์ ํ์๋ค.
The effect of gentamicin on calcium transport in normal rabbit lenses according to
the route of administration
Byung Joo Cho
Department of Medical Science, The Graduate School, Yonsei University
(Directed by Professor Hong Bok Kim)
Endophthalmitis is one of the most catastrophic complications in intraocular
surgery and penetrating injuries of the eye. Recently, gram-negative rods including
pseudomonas have been found to be the major causative organisms of bacterial
endophthalmitis. Gentamicin has been the conventional treatment for pseudomonas
infection and therefore its use for eye infections was evaluated.
Because of poor penetration of antibiotics into the eye and particularly into the
vitreous via the conventional route of administration, the method of injection of
antibiotics directly into either the anterior chamber or the vitreous employed.
Although higher concentration of intraocular gentamicin were obtained,
sight-limiting complications such as cataract and retinal toxicity secondary to
toxic effects on adjacent ocular tissues resulted.
The mechanism of cataract formation have not been proven definitively but the
transparency of the lens is known to depend on its ^^Ca++ level, and many
researchers have proven experimentally that cataracts may occur in cases of
increased lens calcium levels. Recently, in vitro studies with rabbit lenses have
showed that increased calcium levels in cataracts were the results of decreased
active Ca**++ efflux from the lens secondary to inhibition of Ca**++ -activated
ATPase activity in the lens.
In this experiment, gentamicin and normal saline were administrated into the
anterior chamber and into the vitreous. Ca**++-activated ATPase activity in the
lens, active Ca**++ efflux from the lens and calcium concentration in the lens were
measured at day 1, day 3 and 7 days after injection of gentamicin or saline
solution into the anterior chamber or vitreous to compare the effect on calcium
transport in the rabbit lenses according to the route of administration between
four different groups. They included normal saline injection group(control group)
and gentamicin injection group(experimental group), intracameral gentamicin
injection group and intravitreal gentamicin injection group.
The results are summarized as follows;
1. The Ca**++-activated ATPase activity of the lens showed no interval change in
the control group, but progressively inhibited in the experimental group.
2. The active efflux of Ca**++ from the lens was significantly decreased in the
experimental group compared to the control group.
3. The calcium concentration in the lens showed no interval change in the control
group, but progressively increased in the experimental group.
4. The inhibition of Ca**++-activated ATPase activity of the lens, decreased
active Ca**++ efflux from the lens and increased calcium concentration in the lens
were more severe in the intravitreal injection group than intracameral injection
group.
From the above results, it may be speculated that the decreased active efflux of
Ca**++ from the lens induced by gentamicin is related to inhibiting
Ca**++-activated ATPase activity of the lens and this makes the accumulation of
calcium in the lens. These effects were more severe in the intravitreal gentamicin
injection group than in the intracameral gentamicin injection group.
[์๋ฌธ]
Endophthalmitis is one of the most catastrophic complications in intraocular surgery and penetrating injuries of the eye. Recently, gram-negative rods including pseudomonas have been found to be the major causative organisms of bacterial
endophthalmitis. Gentamicin has been the conventional treatment for pseudomonas infection and therefore its use for eye infections was evaluated.
Because of poor penetration of antibiotics into the eye and particularly into the vitreous via the conventional route of administration, the method of injection of antibiotics directly into either the anterior chamber or the vitreous employed.
Although higher concentration of intraocular gentamicin were obtained, sight-limiting complications such as cataract and retinal toxicity secondary to toxic effects on adjacent ocular tissues resulted.
The mechanism of cataract formation have not been proven definitively but the transparency of the lens is known to depend on its ^^Ca++ level, and many researchers have proven experimentally that cataracts may occur in cases of increased lens calcium levels. Recently, in vitro studies with rabbit lenses have showed that increased calcium levels in cataracts were the results of decreased active Ca**++ efflux from the lens secondary to inhibition of Ca**++ -activated ATPase activity in the lens.
In this experiment, gentamicin and normal saline were administrated into the anterior chamber and into the vitreous. Ca**++-activated ATPase activity in the lens, active Ca**++ efflux from the lens and calcium concentration in the lens were
measured at day 1, day 3 and 7 days after injection of gentamicin or saline solution into the anterior chamber or vitreous to compare the effect on calcium transport in the rabbit lenses according to the route of administration between four different groups. They included normal saline injection group(control group) and gentamicin injection group(experimental group), intracameral gentamicin injection group and intravitreal gentamicin injection group.
The results are summarized as follows;
1. The Ca**++-activated ATPase activity of the lens showed no interval change in the control group, but progressively inhibited in the experimental group.
2. The active efflux of Ca**++ from the lens was significantly decreased in the experimental group compared to the control group.
3. The calcium concentration in the lens showed no interval change in the control group, but progressively increased in the experimental group.
4. The inhibition of Ca**++-activated ATPase activity of the lens, decreased active Ca**++ efflux from the lens and increased calcium concentration in the lens were more severe in the intravitreal injection group than intracameral injection
group.
From the above results, it may be speculated that the decreased active efflux of Ca**++ from the lens induced by gentamicin is related to inhibiting Ca**++-activated ATPase activity of the lens and this makes the accumulation of calcium in the lens. These effects were more severe in the intravitreal gentamicin
injection group than in the intracameral gentamicin injection group.restrictio
Refractive State in Childhood with Normal Visual Acuity
์ํ๊ณผ/์์ฌ[์๋ฌธ]
[ํ๊ธ]
์๋ ฅ์ ์๊ธฐ๋ฅ์ ์ฅ์ ๋ฅผ ์ฃผ๋ ์งํ์ด ์์ผ๋ฉฐ ๊ตด์ ์ด์์ด ์๋ ์ ์์์ ๊ฒฝ์ฐ์๋ ํญ์
์ ์์ผ๋ก ๋ํ๋๋ ๊ตด์ ์ด์์ด ์๋ ๋น์ ์์์ด๋ผ๋ ๊ตด์ ์ด์์ ์ข
๋ฅ์ ์ ๋์ ๋ฐ๋ผ์๋
์ ์์ผ๋ก ๋ํ๋ ์ ์๋ค.
์ด์ ์ ์๋ 1985๋
11์ 1์ผ๋ถํฐ 1986๋
3์ 30์ผ ์ฌ์ด์ ์ฐ์ธ๋ํ๊ต ์๊ณผ๋ํ๋ถ์ ์
๋์ธ๋ธ๋์ค๋ณ์ ๋ฐ ์ธ๋ธ๋์ค๋ณ์ ์๊ณผ ์ธ๋ ๋ด์ํ์์ค ์๋ ฅ๊ณผ ๊ตด์ ์ํ์ ์ฅ์ ๋ฅผ ์ค๋งํ
์งํ์ด ์์ผ๋ฉฐ Snellen์ ์์๋ ฅํ์ ์ํ ์๋ ฅ๊ฒ์ฌ๋ก ์์์ ๋์์๋ ฅ์ด ๊ฐ๊ฐ 20/20์ด
์์ธ 6์ธ์์ 15์๊น์ง์ ํ์ 190๋ช
380์์ ๊ด์ฐฐ๋์์ผ๋ก ํ์ฌ ํ๊ฐ์ ๊ตด์ ๊ฒ์ฌ์ ์๊ฐ
์ ๊ตด์ ๊ฒ์ฌ๋ฅผ ์กฐ์ ๋ง๋น์ ์ ์์ ๊ณผ ์ ์ํ์ ์ํํ์ฌ ๋ค์๊ณผ ๊ฐ์ ๊ฒฐ๋ก ์ ์ป์๋ค.
1. ์๊ฐ์ ๊ตด์ ๊ฒ์ฌ์, ์กฐ์ ๋ง๋น์ ์ ์์ ์๋ 147์(38.7%)์ด ๋น์ ์์์ผ๋ก ํ๋ช
๋์
๋๋ฐ, ๊ทธ ์ข
๋ฅ๋ ์์์ฑ๋จ๋์(50.3%), ์์ (37.4%), ๊ทผ์์ฑ๋จ๋์ (6.8%), ์์์ฑ๋ณต๋
์ (5.4%) ์์ด์์ผ๋ฉฐ, ์กฐ์ ๋ง๋น์ ์ ์ํ์๋ 183์(48.2%)์ด ๋น์ ์์์ผ๋ก ํ๋ช
๋์๊ณ
, ์์์ฑ๋จ๋์ (49.7%), ์์ (38.3%), ์์์ฑ๋ณต๋์(8.7%), ๊ทผ์์ฑ๋จ๋์ (3.2%) ์์ด
์๋ค.
2. ๋์๋ฅผ ์ง๋์์ ๋๋์๋ก ๊ตฌ๋ถํ๋ฉด ์ง๋์๊ฐ ๋๋์๋ณด๋ค ์ฝ 2.5๋ฐฐ ๋ง์๋ค.
3. ๊ตด์ ์ด์ ์ ๋๋ ์กฐ์ ๋ง๋น์ ์ ์์ ์๋ ํ๊ท 0.12 D.์๊ณ , 12์ธ์์ ๊ฐ์ฅ ๊ฐํ์์ผ
๋ฉฐ(0.23 D.), ์กฐ์ ๋ง๋น์ ์ ์ํ์๋ ํ๊ท 0.22 D.์๊ณ , 8์ธ์์ ๊ฐ์ฅ ๊ฐํ์๋ค(0.42 D.)
.
4. ์์์ ์ ๋๋ ์กฐ์ ๋ง๋น์ ์ ์์ ์๋ ๋ชจ๋์์ 1.00 D. ์ดํ ์์ผ๋ฉฐ, ์กฐ์ ๋ง๋น์
์ ์ํ์๋ ๋๋ถ๋ถ (93%)์์ 1.00D. ์ดํ์๋ค.
5. ๋์์ ์ ๋๋ ๋๋ถ๋ถ(98 %)์์ 1.00 D.์ดํ์๋ค.
Refractive State in Ckildhood with Normal Visual Acuity
Byung Joo Cho
Department of Medica1 Science The Graduate School Yonsei University
(Directed by Professor Ouk Choi ,M.D.)
To manifest visual acuity of 20/20 or better, the requirements are good optical
imagery, foveal fixation, intact receptor structure and function, and full
integrity of the involved neural pathways. Person with hyperopia of 1-10 diopter or
astigmatism of 1 diopter may be able to reats the line marked 20/20 of snellen's
chart.
Non-cycloplegic and chcloplegic subjective manifest refractions were done on 380
normal eyes with visual acuity of 20/20 or more to observe the prevalence, type and
degree of the refractive errors.
The results were as follows
1. In non-cycloplegic subjective manifest refraction, 147 eyes (38.7%) had
refractive error such as simple hyperopic astigmatism (50.3%), hyperopia (37.4%),
simple myopic astigmatism(6.8%) and compound hyperopic astigmatism(5.4%). In
cycloplegic subjective manifest refraction, 183 eyes (48.2%) were ametropia such as
simple hyperopic astigmatism(49.7%), hyperopia(38.3%), compound hyperopic
astigmatism(8.7%) and simple myopic astigmatism(3.2%).
2. As to the type of astigmatisms, "with the rule" astigmatism was 2.5 times more
than "against the rule".
3. Average degree of refractive errors were 0.12 diopter in non-cycloplegic
refraction and 0.22 diopter in cycloplegic refraction.
4. The degree of hyperopia was less than 1.00 diopter in all cases of
non-cycloplegic refractions and in most cases (93%) of cycloplegic refractions.
5. Degree of astigmatism were less than 1.00 diopter in the majority(98%).restrictio