한국인 상용식품의 플라보노이드 데이터베이스 구축 및 한국 성인의 플라보노이드 섭취량과 항산화능 추정

학위논문 (석사)-- 서울대학교 보건대학원 : 보건학과 보건영양학 전공, 2016. 2. 정효지.Epidemiological studies have suggested that flavonoids exhibit preventive effects on degenerative diseases. However, lack of sufficient data on flavonoid intake have limited evaluating the proposed effects in populations. Therefore, we aimed to estimate the flavonoid intake and antioxidant capacity of flavonoids among Korean adults and determine the major dietary sources of these flavonoids. We constructed a flavonoid database for common Korean foods, based on the food list reported in the 24-hour recall of the KNHANES 2007-2012, using data from the Korea functional food composition table, US Department of Agriculture flavonoid database, Phenol-Explorer database, and other analytical studies. This database, which covers 49% of food items and 76% of food intake, was linked with the 24-hour recall data of 33,581 subjects aged ≥19 years in the KNHANES 2007-2012. The mean daily intake of total flavonoids in Korean adults was 318.0 mg/d, from proanthocyanidins (22.3%), flavonols (20.3%), isoflavones (18.1%), flavan-3-ols (16.2%), anthocyanidins (11.6%), flavanones (11.3%), and flavones (0.3%). The major contributing food groups to the flavonoid intake were fruits (54.4%), vegetables (20.5%), legumes and legume products (16.2%), and beverages and alcohols (3.1%), and food items were apples (21.9%), mandarins (12.5%), tofu (11.5%), onions (9.6%), and grapes (9.0%). In the regression analysis, the consumption of legumes and legume products, vegetables and fruits were the major predictors for total flavonoid intake. In addition, we estimated the antioxidant capacity of flavonoids as 463.0 mg VCE/d. The major dietary sources of antioxidant capacity were fruits (77.6%), mainly grapes, persimmons, and strawberries. The findings of this study could facilitate further investigation on the health benefits of flavonoids and provide the basic information for establishing recommended flavonoid intakes for Koreans.Ⅰ. INTRODUCTION 1 Ⅱ. SUBJECTS AND METHODS 4 Ⅲ. RESULTS 10 Ⅳ. DISCUSSION 32 Ⅴ. REFERENCES 40 Ⅵ. KOREAN ABSTRACT 49Maste

위암에서의 유전자 복제 수와 mRNA 발현 량의 통합적인 분석을 통한 발암유전자 제시

학위논문 (석사)-- 서울대학교 대학원 : 약학과, 2014. 2. 신영기.Gastric cancer is the most common cancer in Asia and remains the second leading cause of cancer mortality in the world. Gastric cancer is usually diagnosed at a very advanced stage. The median survival is ranges from 6-10 months, and 5-year survival rates are <10% in patients with advanced Gastric cancer. Although various studies had been performed to identify the causative genes involved in gastric cancer development, a deeper understanding of the oncogenes that could be characteristic of a variety of histological subtypes and stages of gastric tumor is required. Herein, we report an integrated analysis of genome-wide copy number alteration and global mRNA expression of 40 gastric cancer tissues from 19 elderly and 21 young patients using array-based comparative genomic hybridization (aCGH) and expression microarray. We showed that gastric cancer genomes showed recurrent DNA copy number alterations such as, amplifications at 3q26, 7p11-7p22, 8p11-8q36, 20p11-20p13, and 20q11-20q13, and deletions at 4p11-4p16, 4q11-4q35, 1p35-1p36, 3p21, 8p12, 9p24, and 19p12-19p13. The correlating of transcriptional expression profiling with the aCGH analysis revealed 506 candidate oncogenes showing both overexpression and frequent amplification, and 60 candidate tumor suppressor genes showing both down-regulation and frequent deletion. We identified two regions containing noble target genes. In addition to analyzing Heebo chip and real time PCR, we selected specific cell lines from 14 gastric cell lines. Among our candidate oncogenes, siRNA-based knockdown of CAPZA2, C17orf37, PERLD1, STARD3 and GRB7 within the MET and ErbB2 amplicons in gastric cancer cell lines resulted in a significant suppression of cell proliferation and migration, strongly suggesting that they could be potential oncogenes in gastric cancer. In this study, we suggest that oncogenes and tumor suppressor genes selected by aCGH and microarray, could be potentially used as therapeutic targets.CONTENTS ………………………………………………ii LIST OF FIGURES ……………………………………iv LIST OF TABLES ………………………………………vi INTRODUCTION …………………………………………1 MATERIALS AND METHODS Patients, Tissue Collection and Pathological Review …4 aCGH and gene expression microarray ……………………4 aCGH data analysis ……………………………………………5 Cell culture and reagents …………………………………6 Transfection of cells with siRNA …………………………6 mRNA Analysis (qRT-PCR) …………………………………6 Cell Proliferation assay ……………………………………7 Western blot Analysis ………………………………………7 Transwell Migration assay …………………………………8 RESULTS Comparison in copy number alteration pattern between cancer genomes from young and elderly gastric cancer patients ………………………………………………………9 Array-based CGH Analysis of human gastric cancer genome ……………………………9 Integration analysis array-based CGH and expression array by Patients ……………………11 Relationship between histo-pathological gastric cancer subtypes and gene expression patterns …………………12 Gene expression status in gastric cancer cells ……13 Silencing MET and CAPZA2 expression inhibits proliferation of gastric cancer cell lines ...13 Knock-down of MET and CAPZA2 inhibits the migration of gastric cancer cells …………14 New potential oncogenes within ErbB2 genomic region …15 Synergy effect of Combi-treatment ……………17 DISCUSSION ………………………………………35 REFERANCES …………………………………………………38 국문초록 …………………………………………………42Maste

미스 반 데어 로에의 건축 작품에 나타난 외피의 표현성

학위논문(석사)--서울대학교 대학원 :건축학과,2000.Maste