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    ๋ฐ”์ด์˜คํ•„๋ฆ„์œผ๋กœ ์˜ค์—ผ๋œ ์ƒ์•„์งˆ์˜ ์ ‘์ฐฉ ๊ฐ•๋„์— ์‚ฐ ๋ถ€์‹ ๊ณผ์ •์ด ๋ฏธ์น˜๋Š” ์˜ํ–ฅ

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    ํ•™์œ„๋…ผ๋ฌธ (์„์‚ฌ) -- ์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› : ์น˜์˜ํ•™๋Œ€ํ•™์› ์น˜์˜๊ณผํ•™๊ณผ, 2020. 8. ๊น€์„ ์˜ํ•œ์Šนํ˜„.Objectives. This study aimed to investigate biofilm removal by acid-etching procedures and the effects of residual biofilm on dentin surfaces on composite-dentin adhesion. Materials and Methods. Dentin discs were assigned to five groups: no biofilm formation (C); biofilm formation and no surface treatment (BF); biofilm formation and acid etching (BF-E); biofilm formation and acid etching followed by chlorhexidine soaking (BF-EC); biofilm formation and rubbing with pumice, followed by acid etching (BF-RE). Biofilms were formed on saliva-precoated dentin discs by soaking the discs in Streptococcus mutans (S. mutans) suspension. Biofilm removal from the dentin surface was evaluated quantitatively and qualitatively by confocal laser scanning microscopy and scanning electron microscopy, respectively. To compare the bond strength of biofilm-contaminated dentin with surface treatments, the micro-shear bond strength test was performed with a universal testing machine (LF Plus, Lloyd Instruments, Fareham, UK). Shear force was applied to the bonding interface with crosshead speed 0.5 mm/min. Assessments of micro-shear bond strength and subsequent failure modes were performed. Results. BF-E and BF-EC did not remove the biofilm, whereas BF-RE partially removed the biofilm attached to dentin (P < 0.05). The bond strength of BF-RE was significantly higher than those of BF-E and BF-EC, but lower than that of C-E (P < 0.05). Conclusion. Mechanical biofilm removal is recommended before etching procedures to enhance adhesion to biofilm-contaminated dentin.1. ๋ชฉ์  ๋ณธ ์—ฐ๊ตฌ์˜ ๋ชฉ์ ์€ ๋ฐ”์ด์˜คํ•„๋ฆ„์ด ์‚ฐ ๋ถ€์‹ ๊ณผ์ •์— ์˜ํ•ด ์ œ๊ฑฐ๋˜๋Š”์ง€, ๊ทธ๋ฆฌ๊ณ  ์ƒ์•„์งˆ ํ‘œ๋ฉด์˜ ์ž”์—ฌ ๋ฐ”์ด์˜ค ํ•„๋ฆ„์ด ๋ณตํ•ฉ๋ ˆ์ง„๊ณผ ์ƒ์•„์งˆ ์‚ฌ์ด์˜ ์ ‘์ฐฉ์— ๋ฏธ์น˜๋Š” ์˜ํ–ฅ์ด ์žˆ๋Š”์ง€์— ๋Œ€ํ•ด ์•Œ์•„๋ณด๋Š” ๊ฒƒ์ด๋‹ค. 2. ์žฌ๋ฃŒ ๋ฐ ๋ฐฉ๋ฒ• ์‚ฌ๋žŒ์˜ ์ œ3๋Œ€๊ตฌ์น˜์—์„œ ์–ป์€ ์ƒ์•„์งˆ ๋””์Šคํฌ๋“ค์„ ๋ฐ”์ด์˜คํ•„๋ฆ„ ํ˜•์„ฑ ์œ ๋ฌด์™€ ํ‘œ๋ฉด์ฒ˜๋ฆฌ ๋ฐฉ๋ฒ•์— ๋”ฐ๋ผ 5๊ฐœ์˜ ๊ทธ๋ฃน์œผ๋กœ ๊ตฌ๋ถ„ํ•˜์˜€๋‹ค: 1) ๋ฐ”์ด์˜คํ•„๋ฆ„ ๋ฏธํ˜•์„ฑ(C); 2) ๋ฐ”์ด์˜คํ•„๋ฆ„ ํ˜•์„ฑ ํ›„ ํ‘œ๋ฉด ์ฒ˜๋ฆฌ ํ•˜์ง€ ์•Š์Œ(BF); 3) ๋ฐ”์ด์˜คํ•„๋ฆ„ ํ˜•์„ฑ ํ›„ ์‚ฐ ๋ถ€์‹(BF-E); 4) ๋ฐ”์ด์˜คํ•„๋ฆ„ ํ˜•์„ฑ ํ›„ ์‚ฐ ๋ถ€์‹, ์ดํ›„ ํด๋กœ๋ฅดํ—ฅ์‹œ๋”˜ ์ฒ˜๋ฆฌ(BF-EC); 5) ๋ฐ”์ด์˜คํ•„๋ฆ„ ํ˜•์„ฑ ํ›„ ํผ๋ฏธ์Šค๋ฅผ ์ด์šฉํ•˜์—ฌ ํ‘œ๋ฉด์„ ๋ฌธ์ง€๋ฅธ ํ›„ ์‚ฐ ๋ถ€์‹ ์‹œํ–‰(BF-RE). ํƒ€์•ก์œผ๋กœ ์ฝ”ํŒ…๋œ ์ƒ์•„์งˆ ๋””์Šคํฌ๋ฅผ Streptococcus mutans ๋ถ€์œ ์•ก์— 72์‹œ๊ฐ„ ๋™์•ˆ ๋ฐฐ์–‘ํ•˜์—ฌ ๋ฐ”์ด์˜คํ•„๋ฆ„์„ ํ˜•์„ฑํ•˜์˜€๋‹ค. ์ƒ์•„์งˆ ํ‘œ๋ฉด์—์„œ ๋ฐ”์ด์˜คํ•„๋ฆ„์ด ์ œ๊ฑฐ๋œ ์–‘์ƒ์„ ๊ณต์ดˆ์  ๋ ˆ์ด์ € ์ฃผ์‚ฌ ํ˜„๋ฏธ๊ฒฝ๊ณผ ์ฃผ์‚ฌํ˜• ์ „์žํ˜„๋ฏธ๊ฒฝ์„ ํ†ตํ•ด ํ‰๊ฐ€ํ•˜์˜€๋‹ค. ๋ฐ”์ด์˜คํ•„๋ฆ„์œผ๋กœ ๋ฎํžŒ ์ƒ์•„์งˆ์„ ํ‘œ๋ฉด ์ฒ˜๋ฆฌํ•œ ํ›„ ์ด์— ๋”ฐ๋ฅธ ์ ‘์ฐฉ๊ฐ•๋„๋ฅผ ๋น„๊ตํ•˜๊ธฐ ์œ„ํ•ด ๋งŒ๋Šฅ์žฌ๋ฃŒ์‹œํ—˜๊ธฐ(LF Plus, Lloyd Instruments, Fareham, UK)๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ ๋ฏธ์„ธ ์ „๋‹จ ๊ฐ•๋„ ์‹คํ—˜์„ ์‹œํ–‰ํ•˜์˜€๋‹ค. 0.5 mm/min์˜ ํฌ๋กœ์Šคํ—ค๋“œ ์†๋„๋กœ ์ ‘์ฐฉ๋ฉด์— ์ „๋‹จ๋ ฅ์„ ๊ฐ€ํ•˜์˜€๋‹ค. ๋ฏธ์„ธ์ „๋‹จ์ ‘์ฐฉ๊ฐ•๋„์™€ ํƒˆ๋ฝ๋œ ํ‘œ๋ฉด์˜ ํŒŒ์ ˆ์–‘์ƒ ๋ถ„์„์„ ์‹œํ–‰ํ•˜์˜€๋‹ค. 3. ๊ฒฐ๊ณผ BF-E์™€ BF-EC์—์„œ๋Š” ์ƒ์•„์งˆ์—์„œ ๋ฐ”์ด์˜คํ•„๋ฆ„์ด ์ œ๊ฑฐ๋˜์ง€ ์•Š์•˜๋‹ค. BF-RE์—์„œ๋Š” BF-E์™€ BF-EC์™€ ๋น„๊ตํ–ˆ์„ ๋•Œ์—๋Š” ํšจ๊ณผ์ ์œผ๋กœ ๋ฐ”์ด์˜คํ•„๋ฆ„์ด ์ œ๊ฑฐ๋˜์—ˆ์ง€๋งŒ ์™„์ „ํžˆ ์ œ๊ฑฐ๋˜์ง€๋Š” ์•Š์•˜๋‹ค. BF-RE์—์„œ์˜ ์ ‘์ฐฉ๊ฐ•๋„๊ฐ€ BF-E์™€ BF-EC์—์„œ๋ณด๋‹ค ์œ ์˜ํ•˜๊ฒŒ ๋†’์•˜์ง€๋งŒ C-E๋ณด๋‹ค๋Š” ๋‚ฎ์•˜๋‹ค(P < 0.05) 4. ๊ฒฐ๋ก  ๋ฐ”์ด์˜คํ•„๋ฆ„์œผ๋กœ ์˜ค์—ผ๋œ ์ƒ์•„์งˆ์—์„œ์˜ ์ ‘์ฐฉํšจ์œจ์„ ํ–ฅ์ƒ์‹œํ‚ค๊ธฐ ์œ„ํ•ด ์‚ฐ ๋ถ€์‹ ๊ณผ์ • ์ด์ „์— ๋ฐ”์ด์˜คํ•„๋ฆ„์„ ๋ฌผ๋ฆฌ์ ์ธ ๋ฐฉ๋ฒ•์œผ๋กœ ์ œ๊ฑฐํ•˜๋Š” ๊ฒƒ์ด ์ถ”์ฒœ๋œ๋‹ค.1. Introduction 1 2. Materials and Methods 4 3. Results 13 4. Discussion 15 5. Conclusion 21 6. References 22 Tables and Figures 29 Abstract (in Korean) 35Maste

    ๋งˆ์ด๋ ˆ์•„ ์ฃผํƒ์˜ ๋„๋กœ์ž‰ ๋ถ„์„์„ ํ†ตํ•ด์„œ ๋ณธ ์•Œ๋ฐ” ์•Œํ†  ๊ฑด์ถ•์˜ ์งˆ์„œ์— ๊ด€ํ•œ ์—ฐ๊ตฌ

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :๊ฑด์ถ•ํ•™๊ณผ,2000.Maste
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