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    ๏ง†ๆœ ๅ—ฃๅพทๅธ ๆ™‚ๆœŸ ๅฐไฝ›ํ˜‘์ƒ์—์„œ์˜ ์นดํ†จ๋ฆญ ๋ฌธ์ œ : 1860ยท70๋…„๋Œ€ ๆ–‡็ดณ์˜ ๋ฐ˜๋ฐœ๊ณผ ๊ด€๋ จํ•˜์—ฌ

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :๋™์–‘์‚ฌํ•™๊ณผ,1998.Maste

    ์‹คํ—˜์ ์œผ๋กœ ์œ ๋ฐœํ•œ ๊ฐ€ํ† ์˜ ๊ธ‰์„ฑ ์‹ ๋ถ€์ „์ฆ์—์„œ ์‹ ์žฅ ๋„ํ”Œ๋Ÿฌ ์ €ํ•ญ์ง€์ˆ˜์™€ ํ˜ˆ์ค‘ ํฌ๋ ˆ์•„ํ‹ฐ๋‹Œ์น˜์™€์˜ ์ƒ๊ด€๊ด€๊ณ„์— ๊ด€ํ•œ ์—ฐ๊ตฌ

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :์˜ํ•™๊ณผ ๋ฐฉ์‚ฌ์„ ๊ณผํ•™์ „๊ณต,1995.Maste

    ๊ฐœ์˜ ๊ฒฐ์†๊ณจ ๋ชจ๋ธ์—์„œ ์ž๊ฐ€ํ˜ˆ์ฒญ์œ ๋ž˜ ์•Œ๋ถ€๋ฏผ ์Šค์บํด๋“œ์™€ ๊ฐœ ์ง€๋ฐฉ์œ ๋ž˜ ๊ฐ„์—ฝ์ค„๊ธฐ์„ธํฌ์˜ ๊ณจ ํ˜•์„ฑ ์ด‰์ง„

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    ํ•™์œ„๋…ผ๋ฌธ (์„์‚ฌ)-- ์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› : ์ˆ˜์˜ํ•™๊ณผ, 2014. 2. ๊ถŒ์˜ค๊ฒฝ.Composite synthetic grafts with progenitor cells instead of auto or allografts offer an alternative approach for the fracture repair. The aim of this study was to evaluate osteogenesis of autologous serum-derived albumin (ASA) scaffold seeded with canine adipose tissue-derived mesenchymal stem cells (Ad-MSCs) in canine segmental bone defect model. ASA scaffold was prepared with canine serum by cross-linking and freeze-drying procedures. Beta-tricalcium phosphate (ฮฒ-TCP) was mixed at cross-linking stage of the procedures. Ad-MSCs were seeded into scaffold and incubated for a day before implantation. The grafts were harvested 16 weeks after implantation for histological analysis. Dogs were divided into five groupscontrol, ASA scaffolds with and without Ad-MSCs, ASA scaffolds including ฮฒ-TCP with and without Ad-MSCs. The group of ASA scaffold with Ad-MSCs had larger radiopaque area on radiographs than the groups with ฮฒ-TCP and more bone formation in histological finding than other groups (p < 0.05). It was suggested that Ad-MSCs seeded into ASA scaffold enhanced osteogenesis in bone defect model but ฮฒ-TCP in the ASA scaffold prevented the penetration of the cells related to bone healing. However, more time is required to verify whether fibrous tissues in the middle of the defects could be converted to osteoprogenitor cells.I.INTRODUCTION โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 1 II.MATERIALS AND METHODS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 3 1. AUTOLOGOUS SERUM-DERIVED ALBUMIN SCAFFOLDS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ3 2. PREPARATION OF BETA-TRICALCIUM PHOSPHATE (ฮฒ-TCP) โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ4 3.PREPARATION OF CANINE ADIPOSE-DERIVED MESENCHYMAL STEM CELLS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 4 4.CELL SEEDING โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 5 5.ORTHOTOPIC IMPLANTATION AND HARVEST โ€ฆโ€ฆโ€ฆ 6 6.RADIOGRAPHIC EXAMINATION โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 9 7.HISTOLOGICAL EXAMINATION โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 9 8.STATISTICAL ANALYSIS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 10 III.RESULTS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 11 1.RADIOGRAPHIC FINDINGS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ11 2.HISTOLOGICAL FINDINGS โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ15 IV.DISCUSSION โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 21 V.REFERENCES โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 25 VI.ABSTRACT IN KOREAN โ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆโ€ฆ 31Maste

    ์œ ์†๋ชจํ˜•์„ ์ด์šฉํ•œ ์ถœ๋ ฅ๋„ํ”Œ๋Ÿฌ ์‹ ํ˜ธ๊ฐ•๋„์— ๋Œ€ํ•œ ์‹คํ—˜์  ์—ฐ๊ตฌ

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    ํ•™์œ„๋…ผ๋ฌธ(๋ฐ•์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :์˜ํ•™๊ณผ ์ง„๋‹จ๋ฐฉ์‚ฌ์„ ๊ณผํ•™์ „๊ณต,1998.Docto
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