237 research outputs found
The Effect of Extracellular Collagen on Synthesis of Extracellular Matrix in a 3-Dimensional Culture of Intervertebral Disc Cells
Study Design
In-vitro experimental study.
Objectives
To determine the proteoglycan synthesis of the rabbit nucleus pulposus cells in various concentration of extracellular collagen type I and II under the stimulation of TGF-Ξ²1.
Summary of Literature Review
Therapeutic effect of growth factor and gene therapy can be altered by composition of extracellular matrix. However, the effect of extracellular collagen types I and II on synthetic activity of intervertebral disc cells is not thoroughly studied before.
Materials and Methods
The nucleus pulposus cells were isolated and cultured from 10 skeletally mature rabbits. Cultures were trypsinized and incorporated into alginate beads with different concentration of extracellular collagen type I and II (0.5%, 1.0% and 1.5%). Those cultures with TGF-Ξ²1 (10 ng/ml) served stimulated condition of matrix synthesis. Newly synthesized proteoglycans were assessed by 35S-sulfate incorporation using chromatography on Sephadex G-25 in PD-10 columns. Scintillation count was normalized with DNA content by Hoechst dye method.
Results
In basal condition, difference in proteoglycan synthesis in given concentration of extracellular collagen type I and II were statistically insignificant. In stimulated condition with TGF-Ξ²1, difference in proteoglycan synthesis in given concentration of extracellular collagen type I and II was also statistically insignificant. However, cultures in stimulated condition with TGF-Ξ²1 showed increased amount of newly synthesized proteoglycans compared to those of basal condition regardless of the concentration of extracellular collagen type I and II (p < 0.05).
Conclusion
Anabolic response of rabbit nucleus pulposus cells is relatively insensitive to extracellular matrix composition, which facilitates application of gene therapy in various conditions of disc degeneration.ope
Cytotoxic effect of zinc compound on osteosarcoma cell line
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[μλ¬Έ]Because of the advent of chemotherapy in osteosarcoma limb salvage procedure has become possible and the survival rate of the patients has improved. Yet, there are still problems such as drug resistance and relapse. Multi-drug resistance is one of the major causes of this treatment failure in patients with osteosarcoma. Recent studies have shown that zinc, as a mineral component, has cytotoxic and apoptotic effects on various cancer cell lines of lymphocyte, breast, prostate, ovary, and colon. However, there has been little systematic study regarding the effect of zinc on the osteosarcoma cell line. This study investigates the biological and molecular effects of zinc on the osteosarcoma cell line in vitro study by comparing the osteosarcoma cell with normal fibroblast cell.Primary cultured normal fibroblast (CCD-1120Sk) and osteosarcoma cells (MG-63) were exposed to different concentrations of zinc chloride and zinc-citrate compound. Each was cultured for various time durations. Then, the cell viability was assessed by CCK-8 assay. The cytotoxic effect on cell cycle was evaluated with flow cytometry and the apoptosis was confirmed by the DNA laddering using the agarose gel electrophoresis. I checked the activity of p21WAF1, Bax, Bcl-2, procaspase-3 using the Western blot analysis and caspase-3 activity by ELISA to check the molecular mechanism of apoptosis.The CCK-8 assay revealed that the zinc-citrate compound rather than zinc only, in vitro condition, had distinguishably cytotoxic effects on osteosarcoma cell line in proportion to the dose and time comparing with the normal fibroblast. The flow cytometry and the DNA laddering analysis further showed the apoptosis of the osteosarcoma cells by the particular zinc-citrate compound (0.3mM zinc and 7.5mM citrate). The molecular mechanisms of apoptosis are associated with inactivation of Bcl-2, enhancement of Bax, and activation of caspase-3.In conclusion, this study shows that zinc-citrate compound induces apoptosis of osteosarcoma cells. The zinc-citrate compound is expected to be a new regimen of the chemotherapy although in vivo investigations should follow to ascertain the clinical relevance.ope
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