나노다공성 실리카를 이용한 고 민감도의 면역 진단

학위논문(석사) - 한국과학기술원 : 생명과학과, 2006.8, [ iv, 25 p. ]we developed a GOx immobilized nanoporous MCF silica and magnetic bead based sandwich immuno-detection system. Taking advantage of the unique structural characteristics of the nanoporous MCF silica, the large pore volume and surface area, and mesopores with large diameter and uniform sizes, we successfully immobilized the large enzyme, GOx into the pores using cross-linking agent, also modified the surface of the MCF silica with antibodies. As a result, the MCF-GOx-Ab was used to detecte antigens captured by magnetic bead-Ab through sandwich approach. At optimized conditions, 107beads/mL of magnetic beads, and 10min of catalytic reaction time, using GOx immobilized in MCF silica it could detect model antigens, human IgG from 100pM to 100nM with less nonspecific binding to chicken IgG., and it showed about 20times higher sensitivity than conventional immunoassay using Ab-GOx conjugate antibodies made in our lab. To improve the sensitivity of this immuno detection, several factors still need to be optimized, for instance, the concentration of magnetic beads, the enzyme loading percentage, the quantity of antibodies linked to the surface of MCF silica and the concentrations of the substrates for catalytic reaction. Also different enzymes like HRP (horseradish peroxidase), or ALP (alkaline phosphatase) could be tried to develop a sensitive immunoprobe. As a new immunosensing technology, it must have profound potential not only in detection of various antigens but pathogens as well.한국과학기술원 : 생명과학과

Method for enrichment and desalting of peptides/proteins using magnetic mesoporous carbon foams and its application to mass spectrometric analysis

본 발명은 펩타이드 시료의 효과적인 농축 및 염류제거 방법에 관한 것으로서, 보다 상세하게는 (A) 펩타이드 시료를 나노 다공성 탄소자성체에 흡착시켜 농축하는 농축단계; (B) 시료가 흡착된 나노 다공성 탄소자성체를 세척하여 염류를 제거하는 세척단계; 및 (C) 염류를 제거한 나노 다공성 탄소자성체로부터 흡착되어 농축된 시료를 용출 용매를 사용하여 분리하는 추출단계;로 이루어지는 펩타이드 시료의 농축 및 염류제거 방법에 관한 것이다.본 발명에 의하면 대량의 용액 내에 낮은 농도로 존재하는 펩타이드 시료를 시료의 손상없이 간단히 농축/세척/분리할 수 있어, 펩타이드 시료의 고감도 질량분석 등을 위한 전처리 방법으로 이용될 수 있다. 나노다공성 탄소, 자성체, 역상분리, 농축, 염류제거, 질량분석, 펩타이드, 단백