Limb Salvage with Low Heat Treated Autobone and Conventional Prosthesis

Limb salvage using a low heat treated autobone and conventional joint prosthesis was done in 12 patients with nine osteosarcomas, one chondrosarcoma, and two malignant spindle cell sarcomas, composed of six proximal femurs and six proximal humeri. The overall union rate of the low heat treated autobone with normal host bone was 91.7%, and the mean union time was 4.6 months (range, 3-7 months) after surgery. The functional result of the proximal femur and proximal humerus were 76.7% and 56.8%, respectively. Complications were included hip dislocation (8.3%), fracture of the low heat treated bone (16.7%), and absorption of the low heat treated autobone in four of six patients of the humerus (66.7%). The five years survival rate of the low heat treated autobone was 83.3% using Kaplan Meier survival analysis. Based on the results of this study, limb salvage with the low heat treated autobone appears to be effective in treating primary bone sarcoma with proven high survival and low complication rate, circumventing complications of allograft bone.ope

Effects of Mutant Cartilage Oligomeric Matrix Protein on the Synthesis of Extracellular Matrix in the Swarm Rat Chondrosarcoma Cell Line

Purpose: To investigate the effects of mutated cartilage oligomeric matrix protein (COMP) on the synthesis of cartilage-specific major matrix proteins of Swarm rat chondrosarcoma chondrocytes. Materials and Methods: The Swarm rat chondrosarcoma chondrocytes transfected with chimeric construct consisting of a mutant gene of human COMP and an amino acid FLAG tag sequence were cultured in agarose gel. Formation of extracellular proteoglycan and type-Ⅱ collagen of the cells were evaluated by immunohistochemical staining and measuring 35S-sulfate incorporation. Results: No difference was observed in type-ll collagen detection among the cell line expressing mutant COMP and control cell lines. Histochemical staining of sulfated proteoglycans with safranin-0 showed lower amounts of proteoglycans were incorporated into the extracellular matrix of chondrocytes transfected with mutant gene. 35S-sulfate incorporation into the cell/matrix fractions demonstrates marked lower radiolabel incorporation compared to control cells. Conclusion: Mutation of COMP impacts the processing of proteoglycans rather than type-Ⅱ collagen in three-dimensional culture of Swarm rat chondrosarcoma chondrocytes.ope

The Regional distribution of smooth muscle fibers and growth factors, and their changes following castration in the rat ventral prostate

학위논문(박사)--서울대학교 대학원 :의학과 비뇨기과학전공,1998.Docto

동물 Mucin과 인체 타액의 점도와 습윤성

Thesis(doctor`s)--서울대학교 대학원 :치의학과 구강내과·진단학 전공,2006.Docto

복잡한 지형에서 대기 난류의 특성 분석

학위논문(석사)--서울대학교 대학원 :대기과학과,1998.Maste

Comparison of autologous transfusion with homologous transfusion in spinal arthrodesis : Postoperative infection and clinical re

의학과/석사[한글] 자가 수혈은 감염 등의 동종 수혈의 부작용을 예방한다고 주장되어 왔으며 일반 정형외과 수술이나 고관절 인공관절 치환술 시 자가 수혈과 동종 수혈의 결과를 비교한 연구는 있었다. 그러나, 상대적으로 수술 시간이 길고 실혈량이 많은 척추 유합술에 국한하여 자가 수혈과 동종 수혈간의 술 후 결과를 비교한 연구는 적었다. 본 연구에서는 척추 유합술을 시행 받은 환자를 대상으로 동종 수혈과 자가 수혈의 술 후 감염 및 임상적 결과를 비교하였다. 1999년 1월부터 2000년 12월까지 감압술 후 내고정 기기 고정 및 자가골 이 식술에 의한 척추 유합술 시 수혈을 받은 환자를 대상으로 하였으며 자가 수혈만을 시행한 30예(자가 수혈군)와 동종 수혈만을 시행한 32예(동종 수혈군)로 구분하여 비교하였다. 성별, 연령, 수술 전 및 후 3일의 혈액학적 검사, 분절당 수술 시간, 분절당 실혈량, 분절당 수혈량, 총수혈량, 항생제의 사용 기간을 비교하였다. 술 후 결과를 비교하기 위하여 창상 감염, 요로 감염, 폐렴, 붕소염, 바이러스성 질환의 술 후 감염 빈도를 조사하고 술 후 발열 여부, 발열 기간, 임상적 증상의 호전 정도, 골 유합 및 수혈 처치에 대한 만족도를 조사하였다. 통계 분석은 윈도우용 SPSS 통계분석 프로그램 10을 이용하였고 p 값이 0.05 이하일 때 통계적 유의성이 있는 것으로 판정하였다. 자가 수혈군과 동종 수혈군을 비교 시 성별, 연령, 혈액학적 검사, 분절당 수술 시간, 분절당 실혈량, 분절당 수혈량, 항생제 사용 기간에서 통계학적 차이가 없었으나, 총수혈량은 동종 수혈군에서 많았다. 술 후 감염 빈도, 발열 빈도는 차이가 없었으나, 발열 기간은 동종 수혈군에서 길었다. 수술 후 임상적 증상의 호전 정도 및 골 유합 유무에서는 차이가 없었으나, 수혈 처치에 대한 만족도는 동종 수혈군에서 감소하였다. 자가 수혈이 술 후 감염을 감소 시키지는 않으나 총수혈량과 술 후 발열 기간을 감소시켜 수혈 처치의 불편감을 해소하여 척추 유합술의 목적인 정상 생활로의 복귀에 도움을 주는 것으로 사료된다. -------------------- 핵심 되는 말 : 척추 유합술, 자가 수혈, 임상적 결과, 술 후 감염 [영문] Autologous transfusion has been suspected to overcome the adverse effects of homologous transfusion, such as transmissions of infection. Clinical studies have been designed to examine the relationship between autologous and homologous transfusion and postoperative results in those undergoing general orthopaedic surgeries and total hip arthroplasties. But, few studies have been designed to examine the relationship between autologous and homologous transfusion and postoperative infection, specially in those undergoing spinal fusion which has longer duration of operative procedure and larger amount of blood loss. The purpose is to determine whether there is a relationship between autologous and homologous transfusion and postoperative infection and clinical results of spinal fusion. We reviewed the medical records of all patients who underwent instrumented spinal fusion with autogenous bone graft and received transfusion between January 1999 and December 2000. In 30 procedures patients received autologous blood only, in 32 procedures patients received homologous blood only. We collected information on the patient's gender, age, hemoglobin, hematocrit, platelet count at preoperative and postoperative 3rd day, segmental time in surgery, segmental estimated blood loss, segmental transfused units, total transfused units and duration of antibiotic therapy. We also collected information on the postoperative infections including wound infection, pneumonia, urinary tract infection, cellulitis, and viral transmitted disease and incidence and duration of fever. We also collected information on the improvement of postoperative clinical results, fusion rates and satisfaction with the transfusion. Statistical analysis was performed with software of SPSS 10 and p values that were less than 0.05 were considered significant. Our autologous and homologous recipients were similar to each other in measures such as gender, age, hematologic features, segmental time in surgery, segmental estimated blood loss, segmental transfused units and duration of antibiotic therapy. Homologous transfusion was associated with increased total number of units transfused. There are a significantly increased number of total units transfused in homologous transfused group. No differences were found in the postoperative infection, incidence of fever, but homologous transfusion was associated with longer duration of fever. No differences were found in the improvement of postoperative clinical results, fusion rates, but autologous transfusion was associated with increased satisfaction with the transfusion. Our finding suggested that autologous transfusion dose not result in decreased incidence of postoperative infection. But, the use of autologous blood transfusions may enhance the recovery to the activity of daily living by increasing satisfaction for the transfusion due to decreased number of total units transfused and decreased duration of postoperative fever.ope

(The) role of AITR and AITRL in the lumbar disc herniation

의학과/박사[한글] 요추 추간판 탈출증은 정형외과 질환 중 가장 흔하며 요통 및 하지 방사통을 유발한다. 추간판 탈출증에서 병리 기전은 기계적인 압박, 염증 반응, 자가 면역 반응, apoptosis 등으로 보고된다. 자가 면역 반응의 발생 기전에서 중요한 역할을 하는 면역 조절 T 세포(regulatory T cell)에서 발견된 공동 자극 분자(costimulatory molecule)로서 세포 표면 수용체인 AITR(human, activation inducible tumor necrosis factor receptor[TNFR], GITR : murine, glucocorticoid induced TNFR)이 최근 주목 받고 있다. 동물 실험 결과에서 GITR에 신호를 보내는 항진성(agonistic) anti-GITR 단클론항체를 주입하면 면역 조절 T 세포의 기능이 저하되어 자가 면역 증상이 유발되었으며, 다량의 AITRL(AITR ligand)이 자가 면역 질환의 일종인 류마티스 관절염 환자의 관절 활막 세포에 발현되는 것이 관찰되었다. 따라서, AITR과 AITRL은 추간판 탈출증과 같은 자가 면역 질환의 병태 생리와 연관성이 있을 것으로 추정되나, 현재까지 요추 추간판 탈출증과 관련한 AITR과 AITRL의 발현 특성에 대한 연구는 이루어지지 않았다. 따라서 본 연구는 요추 추간판 탈출증 환자의 추간판 조직과 혈액에서 AITR과 AITRL의 발현 특성을 조사하고자 하였다. 대상 환자를 요추 추간판 탈출증 환자군 (실험군, 20예)과 척추 골절 환자군 (대조군, 5예)으로 분류하여 비교하였다. 추간판 탈출증 환자의 추간판 조직 및 말초 혈액으로부터 분리된 세포에서의 AITR과 AITRL의 발현 특성을 유세포분석 (flow cytometric analysis), 공초점 형광 현미경(confocal laser fluorescent microscope), 면역조직화학염색법(immunohistochemistry), reverse transcriptase-polymerase chain reaction(RT-PCR), enzyme-linked immunosorbent assay (ELISA)을 이용하여 분석하였다. 유세포분석, 공초점 형광 현미경, 면역조직화학염색법으로 조사한 결과 실험군이 대조군보다 AITR과 AITRL의 발현이 증가하였다. AITRL의 mRNA는 실험군에서는 발현이 관찰되었으나, 대조군에서는 관찰이 되지 않았고 AITR의 mRNA는 실험군 및 대조군 모두에서 관찰이 되지 않았다. 혈청내 soluble AITR, soluble AITRL, IL-2, IL-6, IL-8, TNF-？는 실험군이 대조군보다 높았으나, IL-1b 는 차이가 없었다. 요추 추간판 탈출증 환자의 추간판 조직과 혈액에서 AITR과 AITRL의 발현이 증가하였다. [영문]In the lumbar disc herniation the herniated nucleus pulposus may be recognized as a foreign-body by the immune system and this will lead to autoimmune reaction. It was demonstrated that antagonistic monoclonal antibodies specific for costimulatory molecules could be used as novel therapeutic agents to block autoimmune disease progression. Although human activation-inducible tumor necrosis factor receptor (AITR) and ligand for AITR (AITRL) might be considered important costimulatory molecules in the pathogenesis of autoimmune diseases, their examination in lumbar disc herniation remains unexplored. The purpose is to investigate whether AITR and AITRL might be overexpressed in the lumbar disc herniation.The study population consisted of 20 patients suffering from lumbar disc herniation. Five macroscopically normal discs were obtained from the patients with spinal fracture managed with anterior discectomy as a control group. Peripheral blood samples from the patients and control groups were collected during the same period of this study. The expression levels of AITR and AITRL were investigated by flow cytometric analysis, confocal laser scanning microscopy, immunohistochemistry and reverse transcriptase？olymerase chain reaction (RT-PCR). Serum levels of soluble AITR and AITRL were measured by an enzyme-linked immunosorbent assay.In the flow cytometric analysis of AITR and AITRL the patients with lumbar disc herniation had significant higher levels than did normal controls expressed as percentages of cells from disc tissues and PBMC. We found increased AITR and AITRL expression in confocal laser scanning microscopy, immunohistochemistry from the patients with lumbar disc herniation. We did not find increased AITRL expression in RT-PCR from the normal controls but found it from the patients with lumbar disc herniation. We did not find increased AITR expression in RT-PCR from the normal controls and the patients with lumbar disc herniation. Soluble AITR, AITRL, IL-2, IL-6, IL-8 and TNF-？ were elevated in the patients with lumbar disc herniations but there was no difference of IL-1b in the normal controls and the patients with lumbar disc herniation.AITR and AITRL increased in the herniated disc tissue and peripheral blood of the patients with lumbar disc herniation, but decreased in healthy discs.ope

삼중수소 입자크기에 따른 호흡기 내부피폭에 관한 연구

학위논문(석사)--서울대학교 대학원 :원자핵공학과,1996.Maste