73 research outputs found
Limb Salvage with Low Heat Treated Autobone and Conventional Prosthesis
Limb salvage using a low heat treated autobone and conventional joint prosthesis was done in 12 patients with nine osteosarcomas, one chondrosarcoma, and two malignant spindle cell sarcomas, composed of six proximal femurs and six proximal humeri. The overall union rate of the low heat treated autobone with normal host bone was 91.7%, and the mean union time was 4.6 months (range, 3-7 months) after surgery. The functional result of the proximal femur and proximal humerus were 76.7% and 56.8%, respectively. Complications were included hip dislocation (8.3%), fracture of the low heat treated bone (16.7%), and absorption of the low heat treated autobone in four of six patients of the humerus (66.7%). The five years survival rate of the low heat treated autobone was 83.3% using Kaplan Meier survival analysis. Based on the results of this study, limb salvage with the low heat treated autobone appears to be effective in treating primary bone sarcoma with proven high survival and low complication rate, circumventing complications of allograft bone.ope
Effects of Mutant Cartilage Oligomeric Matrix Protein on the Synthesis of Extracellular Matrix in the Swarm Rat Chondrosarcoma Cell Line
Purpose: To investigate the effects of mutated cartilage oligomeric matrix protein (COMP) on the synthesis of cartilage-specific major matrix proteins of Swarm rat chondrosarcoma chondrocytes.
Materials and Methods: The Swarm rat chondrosarcoma chondrocytes transfected with chimeric construct consisting of a mutant gene of human COMP and an amino acid FLAG tag sequence were cultured in agarose gel. Formation of extracellular proteoglycan and type-β
‘ collagen of the cells were evaluated by immunohistochemical staining and measuring 35S-sulfate incorporation.
Results: No difference was observed in type-ll collagen detection among the cell line expressing mutant COMP and control cell lines. Histochemical staining of sulfated proteoglycans with safranin-0 showed lower amounts of proteoglycans were incorporated into the extracellular matrix of chondrocytes transfected with mutant gene. 35S-sulfate incorporation into the cell/matrix fractions demonstrates marked lower radiolabel incorporation compared to control cells.
Conclusion: Mutation of COMP impacts the processing of proteoglycans rather than type-β
‘ collagen in three-dimensional culture of Swarm rat chondrosarcoma chondrocytes.ope
The Regional distribution of smooth muscle fibers and growth factors, and their changes following castration in the rat ventral prostate
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Όλ¬Έ(λ°μ¬)--μμΈλνκ΅ λνμ :μνκ³Ό λΉλ¨κΈ°κ³Όνμ 곡,1998.Docto
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Thesis(doctor`s)--μμΈλνκ΅ λνμ :μΉμνκ³Ό ꡬκ°λ΄κ³ΌΒ·μ§λ¨ν μ 곡,2006.Docto
볡μ‘ν μ§νμμ λκΈ° λλ₯μ νΉμ± λΆμ
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Όλ¬Έ(μμ¬)--μμΈλνκ΅ λνμ :λκΈ°κ³Όνκ³Ό,1998.Maste
Comparison of autologous transfusion with homologous transfusion in spinal arthrodesis : Postoperative infection and clinical re
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Autologous transfusion has been suspected to overcome the adverse effects of homologous transfusion, such as transmissions of infection. Clinical studies have been designed to examine the relationship between autologous and homologous transfusion and postoperative results in those undergoing general orthopaedic
surgeries and total hip arthroplasties. But, few studies have been designed to examine the relationship between autologous and homologous transfusion and postoperative infection, specially in those undergoing spinal fusion which has longer duration of operative procedure and larger amount of blood loss. The purpose
is to determine whether there is a relationship between autologous and homologous transfusion and postoperative infection and clinical results of spinal fusion.
We reviewed the medical records of all patients who underwent instrumented spinal fusion with autogenous bone graft and received transfusion between January 1999 and December 2000. In 30 procedures patients received autologous blood only, in 32
procedures patients received homologous blood only. We collected information on the patient's gender, age, hemoglobin, hematocrit, platelet count at preoperative and postoperative 3rd day, segmental time in surgery, segmental estimated blood loss,
segmental transfused units, total transfused units and duration of antibiotic therapy. We also collected information on the postoperative infections including wound infection, pneumonia, urinary tract infection, cellulitis, and viral transmitted disease and incidence and duration of fever. We also collected
information on the improvement of postoperative clinical results, fusion rates and satisfaction with the transfusion. Statistical analysis was performed with software of SPSS 10 and p values that were less than 0.05 were considered significant.
Our autologous and homologous recipients were similar to each other in measures such as gender, age, hematologic features, segmental time in surgery, segmental estimated blood loss, segmental transfused units and duration of antibiotic
therapy. Homologous transfusion was associated with increased total number of units transfused. There are a significantly increased number of total units transfused in homologous transfused group. No differences were found in the postoperative
infection, incidence of fever, but homologous transfusion was associated with longer duration of fever. No differences were found in the improvement of postoperative clinical results, fusion rates, but autologous transfusion was associated with increased satisfaction with the transfusion.
Our finding suggested that autologous transfusion dose not result in decreased incidence of postoperative infection. But, the use of autologous blood transfusions may enhance the recovery to the activity of daily living by increasing satisfaction
for the transfusion due to decreased number of total units transfused and decreased duration of postoperative fever.ope
(The) role of AITR and AITRL in the lumbar disc herniation
μνκ³Ό/λ°μ¬[νκΈ]
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μΈ λ₯λ§ν°μ€ κ΄μ μΌ νμμ κ΄μ νλ§ μΈν¬μ λ°νλλ κ²μ΄ κ΄μ°°λμλ€. λ°λΌμ, AITRκ³Ό AITRLμ μΆκ°ν νμΆμ¦κ³Ό κ°μ μκ° λ©΄μ μ§νμ λ³ν μ리μ μ°κ΄μ±μ΄ μμ κ²μΌλ‘ μΆμ λλ, νμ¬κΉμ§ μμΆ μΆκ°ν νμΆμ¦κ³Ό κ΄λ ¨ν AITRκ³Ό AITRLμ λ°ν νΉμ±μ λν μ°κ΅¬λ μ΄λ£¨μ΄μ§μ§ μμλ€. λ°λΌμ λ³Έ μ°κ΅¬λ μμΆ μΆκ°ν νμΆμ¦ νμμ μΆκ°ν μ‘°μ§κ³Ό νμ‘μμ AITRκ³Ό AITRLμ λ°ν νΉμ±μ μ‘°μ¬νκ³ μ νμλ€. λμ νμλ₯Ό μμΆ μΆκ°ν νμΆμ¦ νμκ΅° (μ€νκ΅°, 20μ)κ³Ό μ²μΆ 골μ νμκ΅° (λμ‘°κ΅°, 5μ)μΌλ‘ λΆλ₯νμ¬ λΉκ΅νμλ€. μΆκ°ν νμΆμ¦ νμμ μΆκ°ν μ‘°μ§ λ° λ§μ΄ νμ‘μΌλ‘λΆν° λΆλ¦¬λ μΈν¬μμμ AITRκ³Ό AITRLμ λ°ν νΉμ±μ μ μΈν¬λΆμ (flow cytometric analysis), 곡μ΄μ νκ΄ νλ―Έκ²½(confocal laser fluorescent microscope), λ©΄μμ‘°μ§ννμΌμλ²(immunohistochemistry), reverse transcriptase-polymerase chain reaction(RT-PCR), enzyme-linked immunosorbent assay (ELISA)μ μ΄μ©νμ¬ λΆμνμλ€. μ μΈν¬λΆμ, 곡μ΄μ νκ΄ νλ―Έκ²½, λ©΄μμ‘°μ§ννμΌμλ²μΌλ‘ μ‘°μ¬ν κ²°κ³Ό μ€νκ΅°μ΄ λμ‘°κ΅°λ³΄λ€ AITRκ³Ό AITRLμ λ°νμ΄ μ¦κ°νμλ€. AITRLμ mRNAλ μ€νκ΅°μμλ λ°νμ΄ κ΄μ°°λμμΌλ, λμ‘°κ΅°μμλ κ΄μ°°μ΄ λμ§ μμκ³ AITRμ mRNAλ μ€νκ΅° λ° λμ‘°κ΅° λͺ¨λμμ κ΄μ°°μ΄ λμ§ μμλ€. νμ²λ΄ soluble AITR, soluble AITRL, IL-2, IL-6, IL-8, TNF-οΌλ μ€νκ΅°μ΄ λμ‘°κ΅°λ³΄λ€ λμμΌλ, IL-1b λ μ°¨μ΄κ° μμλ€. μμΆ μΆκ°ν νμΆμ¦ νμμ μΆκ°ν μ‘°μ§κ³Ό νμ‘μμ AITRκ³Ό AITRLμ λ°νμ΄ μ¦κ°νμλ€.
[μλ¬Έ]In the lumbar disc herniation the herniated nucleus pulposus may be recognized as a foreign-body by the immune system and this will lead to autoimmune reaction. It was demonstrated that antagonistic monoclonal antibodies specific for costimulatory molecules could be used as novel therapeutic agents to block autoimmune disease progression. Although human activation-inducible tumor necrosis factor receptor (AITR) and ligand for AITR (AITRL) might be considered important costimulatory molecules in the pathogenesis of autoimmune diseases, their examination in lumbar disc herniation remains unexplored. The purpose is to investigate whether AITR and AITRL might be overexpressed in the lumbar disc herniation.The study population consisted of 20 patients suffering from lumbar disc herniation. Five macroscopically normal discs were obtained from the patients with spinal fracture managed with anterior discectomy as a control group. Peripheral blood samples from the patients and control groups were collected during the same period of this study. The expression levels of AITR and AITRL were investigated by flow cytometric analysis, confocal laser scanning microscopy, immunohistochemistry and reverse transcriptaseοΌolymerase chain reaction (RT-PCR). Serum levels of soluble AITR and AITRL were measured by an enzyme-linked immunosorbent assay.In the flow cytometric analysis of AITR and AITRL the patients with lumbar disc herniation had significant higher levels than did normal controls expressed as percentages of cells from disc tissues and PBMC. We found increased AITR and AITRL expression in confocal laser scanning microscopy, immunohistochemistry from the patients with lumbar disc herniation. We did not find increased AITRL expression in RT-PCR from the normal controls but found it from the patients with lumbar disc herniation. We did not find increased AITR expression in RT-PCR from the normal controls and the patients with lumbar disc herniation. Soluble AITR, AITRL, IL-2, IL-6, IL-8 and TNF-οΌ were elevated in the patients with lumbar disc herniations but there was no difference of IL-1b in the normal controls and the patients with lumbar disc herniation.AITR and AITRL increased in the herniated disc tissue and peripheral blood of the patients with lumbar disc herniation, but decreased in healthy discs.ope
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Όλ¬Έ(μμ¬)--μμΈλνκ΅ λνμ :μμν΅κ³΅νκ³Ό,1996.Maste
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