2 research outputs found
Ornithine decarboxylase(ODC) activity in human colorectal tumors
μνκ³Ό/μμ¬[νκΈ]
λμ₯ μ’
μμ μ λ§ μ¦μ μ λλ₯Ό μμ보λ μννμ νμ§μλ‘λ Proliferating Cell Nuclear Antigen staining, Ki-67, [3H]-Thymidine, Ornithine decarboxylase νμ±λ λ° Protein Kinase Cλ±μ΄ μλ€κ³ μλ €μ Έ μλ€ μ΅κ·Ό μ°λ¦¬λλΌμμλ μμ΄ μμμ μꡬνμ ν¨κ»
λμ₯μμ λ°λ³μ¨μ΄ μ¦κ°λλ μΆμΈμ΄λ©°, λμ₯ μ λ§ μ‘°μ§μ κ³Όλ€ν μ¦μμ΄ λμ₯ μ©μ’
κ³Ό λμ₯μμ λ°μμ μ€μν μκ±΄μ΄ λλ―λ‘ λμ₯ μ λ§ μ€μ μ λλ₯Ό μμ보λ κ²λ μμμ μΌλ‘ λ§€μ° μμκ° μλ€κ³ μκ°λλ€.
Ornithine decarbcxylase (ODC)λ μ λ§ μ‘°μ§μ μ¦μκ³Ό λΆνμ κΌ νμν Polyamine μν©μ±μ μ²μ λ¨κ³μμ μ΄λ§€ ν¨μλ‘μ μμ©νλ©° ODC νμ±λμ μ¦κ°λ λ°μ λͺ¨ν νΉν λ°μ μ΄μ§ λ¨κ³μμ μ’
μ μΈν¬ μ¦μμ μ€μν μν μ νλ κ²μΌλ‘ μλ €μ Έ μλ€. μ΄μ μ μλ λμ₯ μ’
μ νμλ₯Ό λμμΌλ‘ μ’
μ μ‘°μ§κ³Ό μ£Όλ³ μ μ μ λ§ μ‘°μ§μμ ODC νμ±λμ λ³ν μμμ κ΄μ°°νκ³ , μ’
μ μ‘°μ§μ μμ λ³λ¦¬νμ νΉμ±μ λ°λ₯Έ ODC νμ±λλ₯Ό λΉκ΅ λΆμνμμΌλ©°, λμ₯ μ©μ’
μ μ λ§μ¦μ μ λλ₯Ό μμ보λ λμ₯ μ λ§ μ¦μμ νμ§μλ‘μ ODC νμ±λμ μ μ©μ±μ κ΄μ°°νμλ€.
1993λ
1μλΆν° 1994λ
9μκΉμ§ μ°μΈλνκ΅ μκ³Όλν μΈλΈλμ€ λ³μκ³Ό μμ£Όλνκ΅ μκ³Όλν λΆμλ³μμ λ΄μνμ¬ λ³λ¦¬ μ‘°μ§νμ μΌλ‘ νμΈλ λμ₯μ’
μ νμ 37μ λ₯Ό λμμΌλ‘ νμλ€. λμ₯μμ κ²½μ° λμ₯ μ μ μ μ μνν ν 10λΆ μ΄λ΄μ μμ‘°μ§ μ νΈκ³Ό μ£Όλ³ μ μμ‘°μ§ μ νΈμ μ±μ·¨νμ¬ Fujirntoλ±μ΄ μ μν λ°©λ²μ κΈ°μ€μΌλ‘ (14)**C -Ornithineμ΄ λ°μνμ¬ μ 리λλ (14)**CO^^2 μ μμ μΈ‘μ νμ°νμ¬ ODC νμ±λλ₯Ό μΈ‘μ νμλ€. λμ₯ μ©μ’
μ κ²½μ°μλ μ‘°μ§ κ²Έμλ‘ μ±μ·¨ν μ‘°μ§ μ νΈμ μκΈ°μ κ°μ λ°©λ²μ μ¬μ©νμ¬ ODC νμ±λλ₯Ό μΈ‘μ νμλ€.
26μμ λμ μμ‘°μ§μμμ νκ· ODC νμ±λλ 18.31(pmol CO^^2 /hr/mg protein),15μμ λμ μ©μ’
μ‘°μ§μμλ 8.05,25μμ λμ μμ‘°μ§ μ£Όλ³μ μ μ μ λ§ μ‘°μ§μμλ 9.63μΌλ‘, μμ‘°μ§μμμ ODC νμ±λκ° μ©μ’
μ‘°μ§κ³Ό μ£Όλ³ μ μ μ‘°μ§μ λΉνμ¬ ν΅κ³μ μΌλ‘ μμ μκ²
μ¦κ°λ λ°λ©΄, λμ₯ μ©μ’
μ‘°μ§κ³Ό μ£Όλ³ μ μμ‘°μ§μ ODC νμ±λ μ°¨μ΄λ ν΅κ³μ μΌλ‘ μ μνμ§ μμλ€.26μμ λμ μνμ μ€ 1μμμλ μ©μ’
μ‘°μ§μ΄ μ
μ±ν λ³νλ₯Ό 보μλλ° ODC νμ±λκ° 30.42 pmol C0^^2 /hr/mg proteinμΌλ‘ λ§μ΄ μ¦κ°λμλ€. λν λμ₯ μ μ’
μ λλ°ν λμ₯μ μ‘°μ§μ ODC νμ±λκ° λ¨λ
μΌλ‘ μμ‘°μ§λ§ μλ κ²½μ°λ³΄λ€ 1.6λ°°κ°λ μ¦κ°λμλ€. κ·Έλ¬λ μμ‘°μ§μμ μμ ν¬κΈ°, μμ νν, μμ λΆν λ° λ³κΈ°λ ODC νμ±λμ μν₯μ λ―ΈμΉμ§ μμλ€. λ¨μ§ μ±λ³κ³Ό μμ μμΉμ λ°λ₯Έ ODC νμ±λλ₯Ό λΉκ΅ν κ²½μ°μλ μ§μ₯μλ³΄λ€ κ²°μ₯μμμ, μ¬μλ³΄λ€ λ¨μμμ ODC νμ±λκ° κ°κ° 1.8λ°°, 1.6λ°°λ‘ λμλ€. 25μμ λμ ν¨νμμ μ μ μ λ§ μ‘°μ§μ νκ· ODC νμ±λλ 9.63(pmol CO^^2 /hr/mg protein), λμ μ©μ’
νμ 10μμ μ μ μ λ§ μ‘°μ§μ νκ· ODC νμ±λλ 6.45λ‘ λ κ·Έλ£Ήκ°μ μ°¨μ΄λ ν΅κ³μ μΌλ‘ μ μνμ§ μμλ€. λμ₯ μ μ’
μ‘°μ§ 8μμ νκ· ODC νμ±λλ 8.94, λΉ μ μ’
μ± μ©μ’
μ‘°μ§ 7μμ νκ· ODC νμ±λλ 7.03μΌλ‘ μ©μ’
μ μ‘°μ§νμ μ견μ λ°λΌ ODC νμ±λμ μ°¨μ΄κ° μμλ€.
κ²°λ‘ μ μΌλ‘ λμ₯μ μ‘°μ§μ ODC νμ±λλ μ©μ’
μ‘°μ§κ³Ό λμ₯μ μ£Όλ³μ μ μ μ λ§μ‘°μ§μ λΉνμ¬ νμ ν μ¦κ°λμ΄ μμΌλ©° μμ μΉ¨μ€κ³Ό μμ λΆνμ λμλ κ΄λ ¨μ±μ΄ μλ κ²μΌλ‘ 보μ, ODC νμ±λλ λμ₯μμ μ λ§μ€μ μ λμ κΉμ μ°κ΄ κ΄κ³κ° μλ€κ³ μκ°λλ€. μ¦ λμ₯μ μ‘°μ§μ μ€μμ λλ λμ₯ μ©μ’
μ‘°μ§μ΄λ μ μ£Όλ³μ μ μ μ λ§μ¦μ λ₯λ ₯κ³Ό λΉκ΅νμ¬ μλ±ν μ¦κ°λμ΄ μμΌλ©°, λμ₯ μ μ’
μ λλ°ν λμ₯μμΌμλ‘ μμ‘°μ§μ μ¦μμ λκ° λμ₯μ μ’
μ λλ°νμ§ μμ λμ₯μμ λΉνμ¬ λμ κ²μΌλ‘ κ΄μ°°λμλ€. κ·Έλ¬λ λμ₯ μ©μ’
μ‘°μ§μ μ¦μμ λλ μ©μ’
μ£Όλ³μ μ μ μ λ§μ‘°μ§κ³Ό λΉκ΅νμ¬ μ μν μ°¨μ΄κ° μμμΌλ©°, μ©μ’
μ μ‘°μ§νμ μ견μ λ°λΌ ODC νμ±λμ λ³νκ° μμλ€. μμΌλ‘ λ κ΄λ²μν λμ₯ μ©μ’
μ‘°μ§μμμ ODC νμ±λλ₯Ό μΈ‘μ νμ¬ μ©μ’
μ‘°μ§μ μ λ§μ¦μ μ λλ₯Ό κ·λͺ
ν νμκ° μλ€κ³ μκ°λλ€.
[μλ¬Έ]
Ornithine decarboxylase(ODC) is the first and rate-limiting enzyme of polyamine biosynthesis involved in normal and neoplastic cell proliferation. The induction of ODC activity plays an important role in the tumer promoting step of the two stage carcinogenesis model, initiation and promotion. Initially, an excellent correlation between ODC activity and tumor promoting ability was shown in mouse skin carcinogenesis model. Also, ODC levels have shown correlation with the progression from normal mucosa to adenoma and carcinoma, especially in hereditary polyposis syndromes. Recently, elevated ODC activity have been studied as potential markers for human colon cancer.
The purpose of this study was to find correlation bebreen ODC activity and colorectal tumor. We compared ODC activity in tumor tissues and adjacent normal appearing mucosa in patients with colorectal turner.
Subjects consisted of twenty six patients with colorectal cancer and eleven patients with colorectal polyp diagnosed at Severance Hospital Yonsei University College of Medicine and Ajou University Hospital from January, 1993 to September, 1954.
Tissue samples of grossly normal appearing mucosa, polyp and carcinoma in large bowel were obtained from patients who received colectomay or colonoscopic procedure. Tissue samples were frozen immediately in liquid nitrogen stored at -70β, and were analyzed for ODC activity within two weeks. ODC activity was assayed by a radiometric technique in which the amount of (14)**C0^^2 liberated from L-(1-(l4)**C) ornithine was measured according to the method by Fujimoto.
Radioactivity of the (14)**CO^^2 trapped in the filter paper was measured in an aqueous miscible scintillant.
The results were as follows:
1. Mean ODC activities(pmol CO^^2 /hr/mg protein) of cancer, polyp and adjacent normal mucosa were 18.31, 8.05, 9.63, respectively. There was a significant difference in mean ODC activity in cancer tissue compared to polyp and adjacent nornal mucosa.
2. There was no significant difference in mean ODC activity according to the size, age, morphology, differentiation and stage of cancer. But there was a statistical difference in mean ODC activity according to sex and tumor location.
Mean ODC activity of males with cancer and of those with colon cancer was significantly higher than that of females with cancer and of these with rectal cancer.
3. Mean ODC activity of cancer coexistent with adenoma was significantly higher than that of cancer with no coexisting adenoma.
4.In adjacent normal mucosa of cancer and polyp, mean ODC activities were 9.63, 6.45, respectively. There was no statistical difference in mean ODC activity between the two groups.
5. Mean ODC activities in adenomatous polyps and non-adenomatous polyps were 8.94, 7.03, respectively.
In conclusion the ODC activity in cancer tissue of large bowel was markedly increased compared with polyp and normal appearing colonic mucosa. But ODC activity was not correlated with invasion and differentiation of colorectal cancer. These results suggest that the elevation of ODC is correlated with cell proliferation
status of colorectal cancer and reflects changes in the large bowel epithelium toward phenotypic neoplastic expression. Also, the mucosa of colorectal cancer with coexisting adenoma was shown to demonstrate a marked increase in mean ODC activity than that of colorectal cancer without adenoma. But the mucosal proliferation status of polyp was not higher than that of normal mucosa adjacent to polyp and ODC activity had no correlation to the pathologic characteristics of polyps. A broad and larger scale study on ODC activity of colorectal polyps is necessary to help predict cell proliferation of tumor.restrictio