(The) effect of bithionol-s-oxide administration on paragonimus westermani, with special reference to the mitochondria

의학과/박사[한글] [영문] Although many investigators attempted to discover the reason for the lethal effect of bithionol-s-oxide on lung flukes, there are only a few reports of its effect on mitochondria. The author present several interesting results noted following the oral administration of bithionol-s-oxide to dogs which had been infected experimentally with the metacercariae bithionol-s-oxide morphological changes in the adult worms and in the cellular mitochondria were observed by light microscopy as well as by electron microscopy. Materials and Methods There months after feeding the metacercariae of Paragonimus westermani, seven out of 10 dogs were given oral doses of bithionol-s-oxide in the doses of 10mg., 20mg., 30mg., 50mg., 70mg., 75mg., and 90mg. per kg. body weight respectively. The three other dogs used as the control group. The size, shape and any changes in the adult worms were observed. With the slides stained by the ordinary H and E stain method and Heidenhain's iron hematoxylin method, general histological changes in the adult worms were observed. Mitochondira in the visceral organs of the adult worms were studied by light microscopy, after adult worms were fixed in formalin, ferric chloride, Helly's solution or Regaud's solution, and embedded in paraffin, sectioned, and stained by Heidlenhains's iron hematoxylin method or Altmann-Kull method. The submicroscopic detail of mitochondria was observed by HS-6 type electron microscope. the tissues of the adult worm were fixed in 1% osmium tetroxide with pH 7.4 veronal-acetate buffer, embedded in metacrylate, sliced at less than 0.1μ by the ultramicrtome, and examined. Results and Conclusions 1. It was shown that the lethal dose of bithionol-s-oxide for the adult worms ranged from 50 to 70 mg./kg. on the basis of study of the number, mobility and morphological changes in the adult worms in both experimental and control groups. 2. While no differences were noted in histological findings between the experimental groups (from 10 to 30 mg./kg.) and the control group, degenerative changes such as cloudy swelling, hyaline degeneration and karyolysis were observed with doses 50mg./kg. More severe degenerative changes were seen in the groups 70mg. and 90mg./kg, 3. In both experimental and control groups, the shape of mitochondria in the intestinal epithelia of the adult worms was generally round or elliptical. The morphological changes of mitochondria reflecting the different dosages of bithionol-s-oxide were as follows: 10mg./kg.……0.3μ in average diameter, similar to that of the control group. 20mg./kg.……Mitochondria wer swollen to 0.4-0.5μ. 30mg./kg.……Swollen further, then destructed or shrunken. Many irregular-sized Mitochondria(0.1-0.6μ) were seen. 50mg./kg.……Mitochondria disappeared completely. 4. The mitochondria of the testicular and ovarian cells were generally fewer and smaller than those of the intestianl epithelium, and the mitochondrial changes varying with the different doses parallelled with those found in the intestianl epithelium in the experimental group. The mitochondria of egg cells in the uterus and reticulum cells were elliptical, round or rod in shape. There were significant changes after bithionol-s-oxide administration. 5. The structural changes in mitochondria observed by electron microscopy after bithionol-s-oxide administration were as follows: 10mg./kg.……The same as those of the control group. 20 and 30 mg./kg.……Swelling, thining, destruction, and disappearance of the double membranes, degenerative, changes of the cristae such as irregular arrangement, severance, destruction and vacuole formation. These were presumed to be the result of destructive phenomena of the tricarboxylic acid cycle.restrictio

전자상거래 과세에 관한 연구

학위논문(석사)--서울대학교 대학원 :경영학과 회계학전공,1999.Maste

The Effect of Perceived Parenting Style and Psychological Separation on Adaptation to School Life in Middle School Students

본 연구는 중학생이 지각하는 부·모의 양육태도 유형과 심리적 독립성의 수준에 따라 학교적응에 차이를 보이는지를 살펴보고자 하였다. 참가자는 서울소재 남녀공학 중학교에 재학 중인 1, 2, 3학년 학생이었고, 참가 자의 성별은 남학생 458명(46.3%), 여학생 532명(53.7%)이었다. 연구결과, 학교적응에 있어서 중학생이 지각 하는 부·모 양육태도의 주효과가 나타났다. 부·모의 양육태도 유형을 민주적으로 지각한 학생들은 권위적으로 지각하는 학생들보다 학교적응 수준이 높았다. 또한 학교적응에 대해 양육태도 유형과 심리적 독립성 수준에 따른 상호작용 효과가 나타났다. 부·모 모두에서 ‘민주적-낮은 독립성’ 집단이 ‘권위적-높은 독립성’집 단보다 학교적응 수준이 높았고, 아버지의 경우 학교적응의 하위 요인 중 학교교사적응에서 ‘권위적-낮은 독 립성’집단이 ‘권위적-높은 독립성’ 집단보다 학교적응 점수가 높게 나타났다. 이러한 결과들과 관련한 시사 점과 함께 추후연구를 위한 제언을 논의하였다. This study examined the difference in school adaptation by the perceived parenting style and psychological separation. 458 male and 532 female middle school students in Seoul participated in this study. The results showed that there was a main effect in school adaptation level by perceived parenting style. The school adaptation level was higher with students perceiving the parenting style as authoritative than authoritarian. Furthermore, there was a interaction effect in school adaptation level by parenting style and psychological separation: ‘authoritative-low psychological separation’ group showed higher level than ‘authoritarian-high separation’ group. With the psychological separation from father especially, ‘authoritarian-low psychological separation’ group had higher teacher adaptation than the ‘authoritarian-low psychological separation’ group. The current study also discussed the suggestions for future studies and implications