암, 당뇨/비만 및 관절염 치료제 후보물질 효능/약리 평가를 위한 in vivo animal model 기반 구축

암, 당뇨/비만 및 관절염 치료제 후보물질 효능/약리 평가를 위한 in vivo animal model 기반 구축BGM079142

Detection of Microcystin Producing-Cyanobacteria by PCR

본 발명은 마이크로시스틴을 생산하는 남조류에만 특이적으로 존재하는 펩타이드 신세타아제(peptide synthetase) 유전자의 특정부위를 증폭할 수 있는 시발체(primer) 및 상기 시발체를 사용한 중합효소 연쇄 반응(polymerase chain reaction, 이하 'PCR'이라 함) 방법에 의한 마이크로시스틴을 생산하는 남조류 검색방법에 관한 것으로, 본 발명에서 사용되는 PCR 방법은 검색대상인 미생물의 특정 DNA의 염기서열을 선택적으로 증폭시킴으로써 검체 내에 존재하는 매우 적은 숫자의 미생물도 쉽게 검색할 수 있기 때문에 미량의 남조류일지라도 검색하여 수중에 존재하는 마이크로시스틴을 생산하는 남조류의 대량 발생을 초기에 감지하고 예측하여 수질의 적절한 관리를 할 수 있다.마이크로시스틴, 시발체, PC

Anti-Inflammatory Metabolites from the Deep-Sea Sediment-Derived Fungus Cystobasidium laryngis

Marine microorganisms are recognized as an important source for drug discovery because they produce secondary metabolites with different skeleton and diverse bioactivities than terrestrial counterparts [1, 2]. Especially, marine microbes derived from the deep-sea are relatively untapped reservoir of metabolites with structural and biological diversity waiting to be discovered because of lack of technology and the difficulty of collecting samples [3]. In our continuing research for bioactive substances from deep-sea microorganisms, we encountered a marine-derived yeast-like fungus Cystobasidium laryngis isolated from a deep-sea sediment sample. Here, we report the fermentation, isolation, structure elucidation and anti-inflammatory activities of three new and four known phenazine derivatives produced by the deep-sea derived fungus C. laryngis.2

Silymarin의 항염증작용에 관한 연구

학위논문(박사) - 한국과학기술원 : 생물과학과, 2003.2, [ xi, 79 p. ]Silymarin, a polyphenolic flavonoid antioxidant, is known to have anti-inflammatory, hepatoprotective, and anticarcinogenic effects. In the present study, we report anti-septic and anti-atherogenic activity of silymarin. This study showed that silymarin protected mice against LPS-induced sepsis. In this model of sepsis, silymarin improved the rate of survival of LPS-treated mice from 6% to 38%. To investigate the mechanism responsible for anti-septic effect of silymarin, we examined the effect of silymarin on the production of inflammatory mediators, including nitric oxide (NO), Interleukin-1β(IL-1β), prostaglandin $E_2 (PGE_2)$, and tumor necrosis factor-α(TNF-α). Silymarin dose dependently suppressed the LPS-induced production of NO in isolated peritoneal macrophages and RAW 264.7 cells, a murine macrophage-like cell line. Also, LPS-induced production of IL-1β and $PGE_2$ in isolated mouse peritoneal macrophages and RAW 264.7 cells. However, TNF-α production was not affected in LPS-stimulated macrophages. Consistent with these results, mRNA expression of iNOS, IL-1β, and COX-2 was significantly abrogated by silymarin in LPS-stimulated RAW 264.7 cells and TNF-α mRNA level was not affected by silymarin treatment. To further investigate the mechanism responsible for the inhibition of gene expression of inflammatory mediators by silymarin, we examined the effect of silymarin on LPS-induced nuclear factor-κB (NF-κB)/Rel$activation, which regulates various genes involved in immune and inflammatory response. In RAW 264.7 cells, the LPS-induced DNA binding activity of NF-κB/Rel was significantly inhibited by silymarin, and this effect was mediated through the inhibition of the degradation of inhibitory factor-κB-α (IκB-α)$. NF-κB/Rel -dependent reporter gene expression was also suppressed by silymarin in LPS-stimulated RAW 264.7 cells. Further study showed that silymarin suppressed the production of reactive oxygen species generated by $H_2O_2$ in RAW 264.7 cells. C...한국과학기술원 : 생물과학과

New Ophiobolins, Cytotoxic Sesterterpenes from the Marine Fungus Aspergillus flocculosus

Five new sesterterpenes (11

Pharmaceutical Composition for the prevention or treatment of inflammatory disease comprising terrein

본 발명은 테레인(terrein)을 유효성분으로 포함하는 염증성 질환 예방 및 치료용 약학적 조성물에 관한 것으로, 상기 테레인은 대식세포에서 IL-6(interleukin-6) 활성 및 p65 단백질 인산화를 유의적으로 억제하는 효과를 나타냄으로, 상기 테레인을 염증성 질환 예방 및 치료용 약학적 조성물의 유효성분으로 유용하게 사용할 수 있다.ope

복원대상지 수산자원 및 관광활성화를 위한 전문가 워크숍(I)

1. 주  제 : 연안복원을 통한 어촌소득 증대방안 마련 2. 일  시 : 2016년 2월 24일(수) 15:00~18:00 3. 장  소 : 충남연구원 1층 회의실 배경 1. 최근 기후 변화 등으로 천수만· 가로림만 주 양식 품종인 바지락의 주기적 폐사 발생으로기후변화 대체 품종 개발 필요성제기 2. 대체품종으로 새꼬막 양식기술 개발· 보급하여 갯벌활용도를 높이고 양식 품종 다양화 및안정 생산으로 어업인 소득 기반 구축 - 이후 생략- 충남 태안해역 새꼬막 양식가능성시범사업 1. 새꼬막 양식 시범사업 추진배경 2. 그 동안 추진 사항 3. 결과 및 파급효과 - 갯벌생태문화의 유형과 활용 방안 1. 갯벌공동체 '갯살림' 2. 갯벌생태문화 유형들 3. 외국 사례 4. 갯벌의 가치 재인식 - 갯벌생물다양성 및 자원화 1. 생물다양성의 개념 2. 갯벌 저서규조류의 생물다양성 3. 갯벌 저서동물의 생물다양성 4. 갯벌생물·복원의 자원화 가능

New Hydroxylated Rhamnolipids from the Marine-Derived Actinomycete Actinoalloteichus hymeniacidonis

Marine-derived microorganisms are an interesting source for valuable secondary metabolites. Moreover, many natural products from marine microbes were developed as medicines and are being studied to cure incurable diseases and diverse infections. Especially, the genus of Actinomyces has been received significant attention since they produce unexpected and unusual chemical compounds with notable biological activities such as antibacterial, antifungal, antiparasitic, anticancer, antiviral activities. During our ongoing investigation on secondary metabolites from marine microorganisms, the Actinoalloteichus hymeniacidonis strain 179DD2

2-acetylaminofluorene이 macrophage에서 interleukin-1β의 유전자 발현에 미치는 영향

학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ vi, 39 p. ]The effects of 2-acetylaminofluorene (AAF), an arylamide carcinogen, on IL-1 production and IL-1 gene expression were investigated. AAF inhibited IL-1 production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and rat peritonial macrophages. To further characterize the effect of AAF on the gene expression of IL-1β, we assessed the effect of AAF on the expression of IL-1β mRNA using competitive RT-PCR. Treatment of RAW 264.7 cells and mouse splenic macrophages with AAF significantly inhibited the production of IL-1β mRNA. In order to investigate the effects of AAF on the regulation of IL-1β gene expression, the effects of AAF on the activation of transcription factors such as NF-κB, AP-1, CRE, and NF-IL6 were examined using a transient transfection and chloramphenicol acetyltransferase (CAT) assay and electrophoretic mobility shift assay. AAF inhibited the transcriptional activation of NF-κB in LPS-stimulated RAW 264.7 cells. The DNA binding activity of NF-κB was also inhibited by AAF in LPS-stimulated RAW 264.7 cells. These data suggest that inhibition of IL-1β gene expression by AAF is mediated by blocking LPS-stimulated NF-κB activation.한국과학기술원 : 생물과학과

Pharmaceutical Composition for the prevention or treatment of osteoporosis comprising Artemisinin

본 발명은 아르테미시닌을 유효성분으로 포함하는 골다공증 예방 및 치료용 약학적 조성물에 관한 것으로, 상기 아르테미시닌은 조골세포를 증식시키고, 파골세포의 형성 및 활성을 유의적으로 억제하는 효과를 나타냄으로, 상기 아르테미시닌을 골다공증 예방 및 치료용 약학적 조성물의 유효성분으로 유용하게 사용할 수 있다.ope