Design and Implementation of Online Tax Service Hall for State Tax Bureau of Yunnan Province

为切实做到深化纳税服务、提升服务质量,集成我省现有网上办税服务系统，为纳税人提供便捷、高效的网上办税服务，建立统一的网上办税服务平台势在必行。通过整合+集成的方式对现有办税服务系统进行升级和重构，制定网上办税系统的统一开发、管理、准入标准，搭建符合我省实际情况和需要的“网上办税服务厅”，对切实做到减轻纳税人办税成本和办税负担、提高工作效率具有重大的意义。 本文介绍基于JSON(JavaScriptObjectNotation)数据交换格式、Http网络协议、Oracle数据库技术的“网上办税服务厅”的设计与具体实现。出于集成原有办税系统的考虑，采用了B/S的架构设计，把系统严格划分为展现层、...In order to deepen tax services and improve quality of service, it is undoubted imperative that online tax services we now have held should be made into integration, convenient and fast online tax service should be provided to taxpayer, and unified tax service platform online should be established. The tax service system should be reconfigured and upgraded by the method of coordination and integra...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201323123

Expression and purification of mucin 16 and preparation and characterization of anti-mucin 16 monoclonal antibody

目的:在原核生物中表达带有HIS标签的MuCIn 16n端重组蛋白(简称为HIS-MuCIn 16n),制备抗MuCIn 16的单克隆抗体(MAb)。方法:将MuCIn 16基因片段插入原核表达载体PET-32,在大肠杆菌中表达重组蛋白,用亲和纯化方法纯化后免疫bAlb/C小鼠,并进行细胞融合。筛选可稳定分泌抗MuCIn 16抗体的阳性单克隆杂交瘤细胞株,用WESTErnblOT、ElISA、免疫荧光和免疫组化等方法分析和鉴定抗MuCIn16的MAb。结果:表达并纯化了HIS-MuCIn 16n蛋白;筛选出几株可稳定分泌特异性抗人MuCIn 16 MAb的细胞株;挑选出效价高、特异性好的1株进行纯化。获得的抗MuCIn 16 MAb,可用于WESTErn blOT、ElISA、免疫组化、免疫荧光等检测,并鉴定该抗体亚型为Igg1。通过上述免疫学实验,分析了在不同肿瘤细胞中MuCIn 16的表达情况。结论:在原核生物中成功表达和纯化带HIS标签的MuCIn 16n重组蛋白,制备出具有高特异性的抗MuCIn16的MAb。AIM:To generate monoclonal antibodies(mAbs) against mucin 16 using purified recombinant protein of human mucin 16 N terminus with His tag(His-mucin 16N) as the antigen.METHODS: Mucin 16 N terminus was cloned into a prokaryotic expression vector pET-32.His-mucin 16N was then expressed in E.coli and purified by the affinity chromotography.Cell fusion was performed after the BALB/c mice were immunized with the purified His-mucin 16N protein.We screened hybridoma cell strains producing mAbs against mucin 16.The specificity and titer of the antibodies were characterized with ELISA,Western blotting,immunofluorescent and immunohistochemical staining.RESULTS: The recombinant protein of His-mucin 16N was expressed and purified.A few hybridoma cell strains which could secrete specific mAbs against mucin 16 were obtained,and one anti-mucin 16 mAb with good specificity and high titer was selected and purified.The isotype of this anti-mucin 16 mAb was determined as IgG1,which indicated that this anti-mucin 16 mAb could be used for ELISA,Western blotting,immunofluorescent and immunohistochemical staining.The endogenous expression of mucin 16 in various cancer cell lines or tissues was also examined with this anti-mucin 16 antibody by Western blotting and other immunoassays.CONCLUSION: The recombinant protein of His-mucin 16N was expressed and purified successfully,with which we prepared anti-mucin 16 mAb with good specificity and high titer.福建省科技重点项目(2011Y0050);厦门市科技计划创新项目(3502Z20123009

Characterization of NSE monoclonal antibodies and establishment of a double-antibody sandwich ELISA assay

通讯作者，E-mail: xtli@ xmu.edu.cn 作者简介: 丁焕弟( 1985 年－ ) ，女，在读硕士，主要从事抗肿瘤单克 隆抗体及诊断试剂盒的研究，E-mail: dinghuandi1125 @163.com。[中文文摘]目的:制备并鉴定NSE(Neuron-specific enolase)单克隆抗体,建立可检测NSE蛋白的双抗夹心ELISA方法。方法:用本实验室已表达纯化的NSE融合蛋白免疫BALB/c小鼠,采用杂交瘤技术制备单克隆抗体。采用WB、IP、IF、IHC等方法对获得的NSE单抗进行鉴定及亚型检测。利用辣根过氧化物酶标记纯化后的NSE单抗,建立一个可检测NSE蛋白的双抗夹心ELISA法。结果:通过分析和鉴定,选定2株可稳定分泌抗NSE抗体的杂交瘤细胞株,效价达4.2×107～6.5×107,亚型为IgG2b。免疫印迹结果显示,该抗体不仅能识别细胞内源NSE蛋白,还能识别分泌到细胞培养上清液中的NSE蛋白,此外还可用于免疫荧光及免疫组化检测。文中所建立的双抗夹心ELISA法,最低检测极限为8.85 ng/ml。结论:成功获得了效价高、灵敏度好及特异性强的NSE单抗,建立了一个双抗体夹心ELISA检测系统,具有良好的临床应用前景。[英文文摘]Objective: Preparation and characterization of monoclonal antibodies against NSE protein，and establishment of a double-antibody sandwich ELISA assay． Methods: BALB/c mice were immunized by using purified recombinant NSE，and monoclonal antibodies were generated by hydridoma technique． These antibodies were characterized with ELISA，Western blot，Immunofluorescent and Immunohistochemical staining． The isotypes of these antibodies were determined with an antibody isotyping kit． With Horseradish Peroxidase labelled NSE monoclonal antibody，we were able to establish a double-antibody sandwich ELISA to detect NSE protein． Results: Two positive hybridoma cell lines were selected for test，the titers of these two monoclonal antibodies could reach 4． 2 × 107 －6. 5 × 107，and their isotypes were IgG2b． Our NSE antibodies could detect not only endogernous NSE protein from cells，but also secreted NSE protein from cells in culture medium by Western blot，in addition，they could be used for immunofluorescent and immunohistochemical staining． The minimum amount of NSE protein could be detected by this double-antibody sandwich ELISA was 8． 85 ng /ml． Conclusion: Our NSE monoclonal antibodies achieved good sensitivity and specificity with high titers，and we established a doubleantibody sandwich ELISA assay which could be used for clinical test in future．福建省科技重点项目(编号项目No.2011Y0050); 厦门市科技计划项目(No.3502Z20123009

基于生物信息学途径探究载脂蛋白 H 在肝衰竭中的功能及作用机制

【目的】通过生物信息学方法，分析在肝衰竭中高表达的载脂蛋白H（APOH）在疾病发生发展中的功能与机制。【方法】从GEO数据库下载多个芯片数据集（GSE14688、GSE38941和GSE96851），以5筛选出差异表达基因，通过Cytoscape及R对前10位关键基因中的APOH基因进行GO生物学功能富集分析和KEGG信号通路分析，并进一步在慢乙肝相关肝衰竭组织中进行表达验证。【结果】共筛选出2438个差异表达基因，其中，1162个上调基因，1276个下调基因。蛋白质相互作用网络分析发现10个枢纽基因分别为：KNG1、IGF1、SPARC、APOH、CLU、SERPING1、TGFB2、CDC37L1、PCYOX1L和APOOL。通过慢乙肝相关肝衰竭组织验证发现APOH在肝衰竭组织中高表达，GeneMANIA预测APOH和炎症反应有关。GO分析及KEGG分析显示，APOH与补体/凝血级联通路以及碳代谢通路密切相关，并且，与补体C3（complement C3）的表达水平呈明显的正相关性。【结论】APOH在肝衰竭组织中高表达，可能通过补体C3调控补体/凝血级联通路以及碳代谢通路参与肝衰竭的发生发展

基于ADPSO算法的机械臂轨迹规划

焊接机械臂工作路径复杂，对规划轨迹平滑性要求较高，并且规划轨迹需满足各关节运动学约束。提出了带扰动的自适应粒子群（Adaptive particle swarm optimization，ADPSO）算法，可以在满足关节约束条件下规划出时间、能力、跃度最优轨迹。采用5次NURBS曲线插值关节工作路径点，使各关节位置、速度、加速度、跃度曲线均连续光滑。利用ADPSO算法进行多目标最优轨迹规划，首先，将粒子外推思想与粒子群优化（Particle swarm optimization，PSO）算法结合，以增强粒子搜索能力；然后，对搜索所得个体极值与群体极值引入扰动，加快粒子收敛速度。在 Matlab环境下进行仿真分析，对比其他智能算法，ADPSO算法的优化效果更好、优化时效性更快

Nursing of a patient with Sintilimab-induced autoimmune myocarditis (1例信迪利单抗治疗后出现免疫性心肌炎患者的护理)

This paper summarized the nursing management for a patient with autoimmune myocarditis after 2 courses of Sintilimab. The patient received hormonotherapy, immunoregulation and other drug treatment. Comprehensive nursing interventions including illness condition observation, medication nursing for intravenous therapy, guidance on rest and exercise, nutritional support and psychological care were carried out during the treatment. The patient's condition improved and was discharged in good condition. (总结1例信迪利单抗治疗2个疗程后发生免疫性心肌炎患者的护理经验。患者住院期间, 给予激素、免疫调节等药物治疗, 密切观察病情变化, 加强静脉治疗与用药护理, 正确指导患者休息与活动, 增强饮食营养, 做好患者心理护理。经过系统的治疗和护理后, 患者病情好转, 顺利出院。

Amplitude analysis of the decays D0 → π+π−π+π− and D0 → π+π−π0π0*

Using e+e− annihilation data corresponding to an integrated luminosity of 2.93 fb−1 taken at the center-of-mass energy √s = 3.773 GeV with the BESIII detector, a joint amplitude analysis is performed on the decays D0 → π+π−π+π− and D0 → π+π−π0π0 (non-η). The fit fractions of individual components are obtained, and large interferences among the dominant components of the decays D0 → a1(1260)π, D0 → π(1300)π, D0 → ρ(770)ρ(770), and D0 → 2(ππ)S are observed in both channels. With the obtained amplitude model, the CP-even fractions of D0 → π+π−π+π− and D0 → π+π−π0π0 (non-η) are determined to be (75.2 ± 1.1stat. ± 1.5syst.) % and (68.9 ± 1.5stat. ± 2.4syst.)%, respectively. The branching fractions of D0 → π+π−π+π− and D0 → π+π−π0π0 (non-η) are measured to be (0.688 ± 0.010stat. ± 0.010syst.)% and (0.951 ± 0.025stat. ± 0.021syst.)%, respectively. The amplitude analysis provides an important model for the binning strategy in measuring the strong phase parameters of D0 → 4π when used to determine the CKM angle γ(φ3) via the B− → DK− decay

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements