An Efficient Axillary Bud Multiplication System of Acacia mangium × A. auriculiformis

以马大杂种相思组培苗为研究对象,通过筛选最佳的增殖培养基,调整增殖时间,探讨芽体长度、继代次数对增殖的影响,增殖培养对生根的影响,建立马大杂种相思腋芽高效组培增殖体系。结果表明:将健壮的初代培养无菌芽剪成含1个腋芽的茎段,接入最佳增殖培养基(改良MS+6-bA1.5 Mg·l-1+nAA0.1 Mg·l-1+AC0.04g·l-1,添加蔗糖3%和琼脂7 g·l-1)中连续培养7代,7次继代的平均增殖倍数为2.52,增殖时间为35天,单株平均繁殖系数为14.99,且7次继代增殖倍数变化小、增殖芽质量好。将增殖芽的茎段(含2~3个腋芽)接入1/2MS+IbA1.0 Mg·l-1+nAA0.5 Mg·l-1(添加蔗糖3%和琼脂7 g·l-1)进行生根培养,15天生根率可达96.5%,移栽后存活率为95.0%。To build an efficient axillary bud multiplication system of Acacia mangium × A.auriculiformis,the aseptic plantlets of A.mangium × A.auriculiformis were used to screen the optimal protocol for in vitro multiplication.The optimal multiplication medium and period,length of the sterile stems,subculture times,rooting and transplant survival rate were studied.The results showed that after being cultured 7 times on improved-MS + 0.5 mg·L- 16-BA + 0.1 mg·L- 1NAA + 0.04 g·L- 1Ac( with combination of 3% sugar and 7 g·L- 1agar),which was the best multiplication medium screened from the trial test,the average proliferation times of the stem with one axillary bud were 2.52,and the propagation coefficient of the plantlet was around 14.99 after 35 days.Moreover,the plantlets grew well and there was no obvious variation of the multiplication ratio.The aseptic stem with 2 or 3 axillary buds was inoculated to 1 /2MS + IBA1.0mg·L- 1+ NAA0.5 mg·L- 1( with combination of 3% sugar and 7 g·L- 1agar) for rooting culture,the rooting rate was96.5% in 15 day and the transplant survival rate was 95.0%.国家“十二五”科技支撑计划项目(2012BAD01B0402

Tissue Culture Technique of Acacia mangium Elite Trees

作者简介: 黄烈健( 1971—) ，男,博士,副研究员,研究方向为林木遗传育种与森林培育，电话: 020-87033463,E-mail: hlj@ ritf.ac.cnTaking Acacia mangium elite trees as explants，the tissue culture technique system for dormant bud of 3-5 year-old elite trees was established． The system includes the germination-inducing of the dormant buds，the multiplying of shoots，the rooting of adventitious shoots，and the pre-treating and transplanting of seedlings． The medium MS + Sucrose 30 g·L－1 + BA 0.5 mg·L－1 + NAA 0.1 mg·L－1 was used for inducing the dormant buds; the medium MS + BA 1.0 mg·L－1 + NAA 0.05 mg·L－1 was used for multiplying; while the medium 1 /2 MS +IBA 2.0 mg·L－1 + NAA 0.5 mg·L－1 was used for rooting.The results revealed that it was viable to producing field seedlings with micropropagation.Although the branches with dormant buds harbored many kinds of microbes and the adventitious shoots were not easy to root，20% to 30% healthy germination could be yielded and the rooting rate of adventitious shoots could be higher than 85%.Pretreated with ABT powder ( rooting hormone) ，both the rooting rate and survival rate of adventitious shoots were nearly 100%．国家“十一五”科技支撑计划项目(2006BAD01A15-5,2006BAD32B010-3); 农业科技成果转化项目(2009GB24320482

Research Advances on the Tissue Culture of Acacia species

综述了相思树种组织培养技术研究进展,分析存在的主要问题,并结合研究实例提出了相应的解决方法,可为推动相思树种工厂化组培育苗提供参考。On the base of the review of research advances of Acacia species tissue culture,this paper analysed the existed problems of Acacia species,and gave related solving methods by combining with the facts,which might provide references for promoting the industrialized culture and breeding of Acacia species.国家“十二五”科技支撑计划项目(No.2012BAD01B0402