Characterization, Genomic Organization and Expression Analysis of a Novel Anionic Antimicrobial Peptide, Scygonadin, Isolated from Scylla serrata

锯缘青蟹Scyllaserrata(Forskål,1775)是我国最重要的海水养殖经济蟹类之一。本论文运用液相色谱技术分离纯化获得一种新抗菌肽，命名为Scygonadin，利用分子生物学技术克隆获得Scygonadin全长基因序列，利用生物信息学的方法阐明其基因结构，运用分子生物学的方法分析该基因在锯缘青蟹体内各组织器官中的转录表达情况等，为阐明锯缘青蟹的先天性免疫及生殖免疫机制奠定基础。通过研究获得了如下结果：1、分离纯化获得一种新抗菌肽。以锯缘青蟹的精液为材料，通过酸性溶液萃取，联合利用阳离子交换层析和反相高效液相层析技术进行分离纯化，获得一种单成份，Tricine-SDS-...Scylla serrata (Forskål, 1775) is one of the most important economic marine aquaculture species in China. A novel antibacterial peptide named scygonadin has been isolated and purified from the seminal plasma in Scylla serrata using liquid chromatography. The full-length cDNA nucleotide sequence and amino acid sequence of scygonadin were characterized using PCR and RACE. The gene organization...学位：理学博士院系专业：海洋与环境学院海洋系_海洋生物学学号：B20012700

关于完善企业环境会计信息披露制度的思考

随着我国环境问题的日益严重,企业环境会计信息披露成为环境会计理论和实务研究中的一项重要内容。由于认识、技术、制度、人才等方面的原因,在我国企业环境会计信息披露实践中尚存在许多问题。这些问题的解决需要政府、企业、社会的共同努力,加强环境会计信息披露相关法律法规的建设和政府部门的监管力度;建立健全企业环境会计信息系统,从源头上保证环境会计信息的质量;发挥以会计师事务所为主体的社会机构在企业环境会计实践中的支持和监督职能

甲壳动物抗菌肽研究进展

国家863计划资助项目(2002A603013);; 厦门市科技局资助项目(3502Z20021052);; 福建省科技资助项目“福建省海洋生物优良种质和生物活性物质的应用基础研究

拟穴青蟹抗菌肽Crustin新变体的表达特性与抗菌功能

Crustins是一类较早发现并广泛分布在甲壳动物中且富含半胱氨酸的阳离子抗菌肽,能够参与抗细菌的免疫应答。从拟穴青蟹（Scylla paramamosain）中克隆获得Crustin基因,命名为SpCrus1a,其cDNA序列全长744 bp,开放阅读框编码113个氨基酸,成熟肽分子量10.03 kDa,等电点8.30。表达分析结果发现其转录本主要存在于鳃、卵巢、上皮组织中,脂多糖刺激后SpCrus1a会上调表达。体外合成SpCrus1a的第29～47位氨基酸进行抗菌活性实验发现,其对革兰氏阳性菌具有较强的抗菌活性,对被测的革兰氏阴性菌抗菌活性较弱或无抗菌活性。合成肽Sp Crus1a29-47能够在较短时间内杀死绝大多数的金黄色葡萄球菌,2 h内杀死全部的金黄色葡萄球菌。扫描电镜发现合成肽Sp Crus1a29-47可造成金黄色葡萄球菌表面结构崎岖不平,高浓度Sp Crus1a29-47造成细菌的大量死亡。上述结果表明,SpCrus1a是抗菌肽Crustins的新变体。国家自然科学基金(U205123,31172438);;厦门南方海洋研究中心科技项目(14PYY050SF03

试论上市公司高管薪酬的粘性——基于我国金融业上市公司的研究视角

本文以中国股票市场中金融业上市公司为样本,对高层管理人员薪酬粘性问题展开研究。研究发现,高管薪酬存在粘性,即在公司绩效上升时高管薪酬增加幅度显著高于公司绩效下降时的减少幅度。进一步研究还发现,公司规模、股权安排、负债比例等一系列特征对高管薪酬粘性具有显著的影响关系。本文研究不仅揭示我国上市公司高管薪酬变动特征,同时也为学术界进一步研究高管薪酬粘性问题提供理论基础与数据支持

NMR Studies on Interactions between Diperoxovanadate and Imidazole-like Ligands

为探讨咪唑环上取代基团对反应平衡的影响,在模拟生理条件(0.15 mol·L~(-1)NaCl溶液)下,应用多核(~1H、~(13)C和~(51)V)、扩散排序谱(DOSY)以及变温NMR等谱学技术研究双过氧钒配合物NH_4[OV(O_2)_2{2-(2'-Pyri-dine)- [midazole}]·4H_2O(简写为bpV(Imi-Py))和咪唑类配体(咪唑、2-甲基-咪唑、4-甲基-咪唑和组氨酸)的相互作用,其从强到弱的顺序为咪唑≈4-甲基-咪唑>2-甲基-咪唑>组氨酸.研究结果表明,咪唑环上取代基团空间位阻对反应平衡产生较大影响,同时竞争配位的结果导致新的6配位过氧物种[OV(O_2)_2L]~-(L为咪唑类配体)的生成,当配体为4-甲基-咪唑和组氨酸时,生成的则是一对异构体.To understand the substituting effects of imidazole ring on the reaction equilibrium,the interactions between diperoxovanadate complex NH_4[OV(O_2)_2{2-(2'-Pyridine)-Imidazole}]·4H_2O (abbr.bpV(Imi-Py)) and a series of imidazole-like ligands (imidazole,2-methyl-imidazole,4-methyl-imidazole,and histidine) in solution were explored using multinuclear (~1H,~(13)C,and ~(51)V) magnetic resonance,diffusion ordered spectroscopy (DOSY),and variable temperature NMR in 0.15 mol·L~(-1) NaCl ionic medium for mimicking the physiological conditions.The experimental results indicated that the activities of bpV(Imi-Py) and organic ligands were as follows:imidazole≈4-methyl-imidazole>2-methyl- imidazole>histidine.The stedc effect of the organic ligands affects the reaction equilibrium.At the same time,new six- coordinated peroxovanadate species [OV(O_2)_2L]-(L=imidazole-like ligands) were formed due to the competitive coordination between 2-(2'-pyridyl)-imidazole and the imidazole-like ligands.When the ligand was 4-methyl- imidazole or histidine,a pair of isomers was formed.国家自然科学基金(20772027);; 973子课题(2003CB716005);; 湖南省自然科学基金(06JJ30004);; 中国博士后科学基金(20070410805);; 湖南省教育厅青年项目(068028);; 卫生部(福建省)卫生教育联合攻关计划(3502220051027);; 厦门市重大疾病攻关研究基金(Wkj2005-2-019);; 固体表面物理化学国家重点实验室开放基金和湖南科技大学博士基金(E55107)资

NMR and Theoretical Study on Interactions between Diperoxovanadate Complex and Substituted Pyridines

为探讨有机配体上取代基团对反应平衡的影响,在模拟生理条件下(0.15mol/L NaCl溶液),应用多核(1H,13C和51V)多维(DOSY)以及变温NMR技术研究双过氧钒配合物[OV(O2)2(D2O)]-/[OV(O2)2(HOD)]-(简写为bpV)与取代吡啶的相互作用.bpV与有机配体的反应性从强到弱的顺序为:皮考林酸根>异烟酸根>异烟酸甲酯>皮考林甲酯,这说明吡啶环上同一位置上的不同取代基团和同一取代基团在不同位置上都影响反应平衡,竞争配位导致一系列新的6配位(配体为异烟酸根和异烟酸甲酯)或7配位(配体为皮考林酸根和皮考林甲酯)的过氧钒物种[OV(O2)2L]n-(L=取代吡啶,n=1或2)生成,密度泛函计算结果较合理地解释了实验结果,并表明溶剂化在反应中起重要作用.To understand the substitution group effects of organic ligands on the reaction equilibrium,the interactions between diperoxovanadate complex [OV(O2)2(D2O)]-/[OV(O2)2(HOD)]-(abbr. bpV) and a series of substituted pyridines were explored using multinuclear(1H,13C,and 51V) magnetic resonance,DOSY,and variable temperature NMR in 0.15 mol/L NaCl ionic medium for mimicking the physiological condition. The reactivity order among the substituted pyridines and bpV is picolinate>isonicotinate> methyl isonicotinate>methyl picolinate. The competitive coordination results in the formation of a series of new six-coordinated(isonicotinate and methyl isonicotinate) or seven-coordinated(picolinate and methyl picolinate) peroxovanadate species [OV(O2)2L]n-(L=substituted pyridines,n=1 or 2). Both the different substitution groups at the same position and the same groups at the different substitution position affect these reactions. The results of density functional calculations provide someway a reasonable explanation for the relative reactivity of the substituted pyridines. Solvation effects play an important role in these reactions.国家自然科学基金(Nos.20772027,20803020);; 973子课题(No.2003CB716005);; 湖南省自然科学基金(No.06JJ30004);; 中国博士后科学基金(No.20070410805);; 湖南省教育厅青年项目(No.06B028);; 固体表面物理化学国家重点实验室资助项目;; 湖南科技大学博士基金(No.E-55107)资助项目

NMR Studies on Interactions between Diperoxovanadate Complex and 3-Substituted Pyridines

为探讨有机配体上取代基团对反应平衡的影响,在模拟生理条件下(0.15mol/LNaCl溶液),应用多核(1H、13C和51V)多维(DOSY)以及变温NMR技术研究双过氧钒配合物[OV(O2)2(D2O)]-/[OV(O2)2(HOD)]-(简写为dpV)与3-取代吡啶的相互作用,并首次报道了一些物种的NMR化学位移.dpV与有机配体的反应性从强到弱的顺序为:吡啶>烟酸根>烟酸甲酰胺≈烟酸甲酯,这说明吡啶环上取代基影响反应平衡.竞争配位导致一系列新的6配位的过氧钒物种生成.密度泛函计算结果合理地解释了实验结果,并表明溶剂化效应在反应中起重要作用.To understand the substituting group effects of organic ligands on the reaction equilibrium, the interactions between a diperoxovanadate complex [OV(O2)2(D2O)]-/[OV(O2)2(HOD)]- (abbr. dpV) and a series of 3-substituted pyridines in solution were explored using multinuclear (1H, 13C, and 51V) magnetic resonance, DOSY, and variable temperature NMR in 0.15 mol/L NaCl ionic medium for mimicking the physiological condition. Some direct NMR data were given for the first time. The reactivity among the 3-substituted pyridines towards dpV takes the order of pyridine>nicotinate>N-methyl nicotinamide≈ methyl nicotinate. The competitive coordination results in the formation of a series of new six-coordinated peroxovanadate species [OV(O2)2L]n- (L=3-substituted pyridines, n=1 or 2). The results of density func-tional calculations provided a reasonable explanation of the relative reactivity of the 3-substituted pyridines. Solvation effects play an important role in these reactions.国家自然科学基金(Nos.20772027,20803020);; 973子课题(No.2003CB716005);; 湖南省自然科学基金(No.06JJ30004);; 中国博士后科学基金(No.20070410805);; 湖南省教育厅青年项目(No.06B028);; 固体表面物理化学国家重点实验室资助项目;; 湖南科技大学博士基金(No.E-55107)资助项

NMR Studies on Interactions between Diperoxovanadate and 1-Ethyl-1H-Imidazole

为探讨过氧钒配合物中有机配体对反应平衡的影响,在模拟生理条件下(0.15MOl·l-1nACl溶液),应用多核(1H、13C和51V)多维(COSy和HSQC)核磁共振(nMr)以及变温技术等谱学方法研究双过氧钒配合物[OV(O2)2l]-(l=d2O或HOd,与之配位的过氧钒物种简写为bPV)和[OV(O2)2ll′]n{-n=1-2,ll′=3-羟基-皮考啉酸根(3-OH-PIC),2-(2′-吡啶)-咪唑(Py-IM),1,10-邻菲啰啉(PHEn),与它们配位的含钒物种分别简写为bPV(3-OH-PIC)、bPV(Py-IM)和bPV(PHEn)}与n-乙基咪唑(n-ET-IM)的相互作用.实验结果表明,n-ET-IM与4种双过氧钒配合物反应性从强到弱的顺序为bPV>bPV(3-OH-PIC)>bPV(Py-IM)>bPV(PHEn).研究表明,金属中心上配体的配位能力、空间位阻和分子量等因素都对反应平衡产生较大的影响,同时竞争配位的结果导致新的6配位过氧物种[OV(O2)2(n-ET-IM)]-的生成.利用上述谱学方法有助于揭示此类相互作用体系的反应过程和配位方式.To understand the effects of organic ligands on the reaction equilibrium, interactions between a series of diperoxovanadate complexes [OV(O2)2L]- (L=D2O or HOD, the corresponding peroxovanadate species (bpV)) and [OV (O2)2LL′]n-{n=1-2; LL′ =3-hydroxyl-picolinate (3-OH-pic), 2-(2′-pyridine)-imidazole (py-im), 1,10-phenanthroline (phen), the corresponding peroxovanadate species bpV(3-OH-pic), bpV(py-im), and bpV(phen)}and 1-ethyl-1H-imidazole (N- Et-im) in solution were explored using multinuclear (1H, 13C, and 51V) magnetic resonance, COSY (correlated spectroscopy), HSQC (heteronuclear single quantum coherence) and variable temperature nuclear magnetic responance (NMR) using 0.15 mol·L-1 NaCl ionic medium to mimic physiological conditions.Experimental results indicated that the reactivity of these four complexes with 1-ethyl-1H-imidazole decreased as follows: bpV>bpV(3-OH-pic)>bpV(py-im)> bpV(phen).The coordinating ability, the steric effect, and the molecular weight of these organic ligands affected the reaction equilibrium.A new six-coordinated peroxovanadate species [OV(O2)2(N-Et-im)]- was formed because of competitive coordination.国家自然科学基金(20772027;20803020);中国博士后科学基金(20070410805);卫生部科学研究基金-福建省卫生教育联合攻关计划(WKJ2008-2-036);厦门市重大疾病攻关研究基金(3502Z20051027)资助项目---

Purification and part characterization of a novel antibacterial protein Scygonadin, isolated from the seminal plasma of mud crab, Scylla serrata (Forskål, 1775)

【英文摘要】A novel protein was isolated from the seminal plasma of the mud crab, Scylla serrata (Forskål, 1775). It exhibited an antibacterial activity against the Gram-positive bacterium Micrococcus leteus with IC90 of 0.125 mg/ml. The extraction procedure for the protein included techniques of acid extraction, ion-exchange chromatography on SP-Sepharose Fast Flow and reverse-phase liquid chromatography on Source 5R RPC. It showed a molecular mass of 10.8 kDa by SDS-PAGE. A partial 20 residue NH2-terminal sequence was determined by Edman degradation and MS-fingerprint of the protein was conducted. Similarity search in protein databases (BLAST) revealed that the protein exhibited no significant homology to any other reported antimicrobial peptides. We propose the name Scygonadin (from the gonad of S. serrata) for this antibacterial protein