α-羟丁酸脱氢酶与乳酸脱氢酶比值的临床意义

目的探讨α-羟丁酸脱氢酶与乳酸脱氢酶比值对心肌梗死的诊断价值。方法采用470例血清HBDH升高患者血清,分为心肌梗死组、脑部疾病组、肺部疾病组、肝脏疾病组、肾脏疾病组,测定血清中α-羟丁酸脱氢酶与乳酸脱氢酶比值。同时,设正常对照组。结果心肌梗塞组及脑部疾病组HBDH/LDH比值明显升高,与正常对照组有着显著性差异(P0.05)。结论以HBDH/LDH的比值诊断心肌梗塞较之单纯用HBDH或LDH诊断特异性高

The effect of kaempferol on COX-2and iNOS expression and generated production in LPS-induced RAW 264.7 cells

目的探讨山萘酚对脂多糖(lIPOPOlySACCHArIdES,lPS)诱导rAW 264.7细胞COX-2及InOS表达的影响。方法四氮唑盐法(MOnOTE-TrAzOlIuM TEST,MTT)检测山奈酚对rAW264.7细胞生长增殖的影响,放射免疫测定法(rIA)检测山萘酚对PgE2和nO生成的影响,免疫印迹法(WESTErn blOTTIng)检测COX-2及InOS蛋白的表达。结果山萘酚抑制lPS诱导的rAW 264.7细胞PgE2和nO的生成,同时下调lPS诱导的rAW 264.7细胞COX-2及InOS蛋白的表达。结论山萘酚抑制2个诱导酶COX-2和InOS的表达,从而减少炎性产物PgE2和nO的生成,这可能是山萘酚抗炎的机制之一。Objective To study the effect of kaempferol on COX-2and iNOS expression in LPS-induced RAW 264.7cells.Methods The effects of kaempferol on the proliferation in RAW264.7cells were evaluated by monote-trazolium test assay(MTT);the effect o f kaempferol on the product of PGE2and NO in RAW264.7cells was measured by radioimmunoassay(RIA);the effects of kaempferol on the expressions of COX-2and iNOS protein in RAW264.7cells were analyzed by Western blot.Results Kaempferol inhibited the LPS-induced PGE2and NO synthesis in RAW 264.7cells.The protein expression of COX-2and iNOS in RAW264.7cells were also decreased by kaempferol.Conclusions The results show that kaempferol reduces the augmented biosynthesis of PGE2and NO by inhibiting the protein expression of COX-2and iNOS in RAW264.7cells.The finding may partly explain the anti-inflammatory effect of kaempferol.广东省社会发展领域科技计划项目(53025); 广东省重点扶持学科项目(GX0307