Duck TRIM29 negatively regulates type I IFN production by targeting MAVS

The innate immune response is a host defense mechanism that induces type I interferon and proinflammatory cytokines. Tripartite motif (TRIM) family proteins have recently emerged as pivotal regulators of type I interferon production in mammals. Here, we first identified duck TRIM29, which encodes 571 amino acids and shows high sequence homology with other bird TRIM29 proteins. DuTRIM29 inhibited IFN-β and IRF7 promoter activation in a dose-dependent manner and downregulated the mRNA expression of IFN-β, IRF7, Mx and IL-6 mediated by duRIG-I. Moreover, duTRIM29 interacted and colocalized with duMAVS in the cytoplasm. DuTRIM29 interacted with duMAVS via its C-terminal domains. In addition, duTRIM29 inhibited IFN-β and IRF7 promoter activation and significantly downregulated IFN-β and immune-related gene expression mediated by duMAVS in ducks. Furthermore, duTRIM29 induced K29-linked polyubiquitination and degradation of duMAVS to suppress the expression of IFN-β. Overall, our results demonstrate that duTRIM29 negatively regulates type I IFN production by targeting duMAVS in ducks. This study will contribute to a better understanding of the molecular mechanism regulating the innate immune response by TRIM proteins in ducks

Plasma-Catalytic CO₂ Hydrogenation over a Pd/ZnO Catalyst: <i>In Situ</i> Probing of Gas-Phase and Surface Reactions

Plasma-catalytic CO2 hydrogenation is a complex chemical process combining plasma-assisted gas-phase and surface reactions. Herein, we investigated CO2 hydrogenation over Pd/ZnO and ZnO in a tubular dielectric barrier discharge (DBD) reactor at ambient pressure. Compared to the CO2 hydrogenation using Plasma Only or Plasma + ZnO, placing Pd/ZnO in the DBD almost doubled the conversion of CO2 (36.7%) and CO yield (35.5%). The reaction pathways in the plasma-enhanced catalytic hydrogenation of CO2 were investigated by in situ Fourier transform infrared (FTIR) spectroscopy using a novel integrated in situ DBD/FTIR gas cell reactor, combined with online mass spectrometry (MS) analysis, kinetic analysis, and emission spectroscopic measurements. In plasma CO2 hydrogenation over Pd/ZnO, the hydrogenation of adsorbed surface CO2 on Pd/ZnO is the dominant reaction route for the enhanced CO2 conversion, which can be ascribed to the generation of a ZnO x overlay as a result of the strong metal-support interactions (SMSI) at the Pd-ZnO interface and the presence of abundant H species at the surface of Pd/ZnO; however, this important surface reaction can be limited in the Plasma + ZnO system due to a lack of active H species present on the ZnO surface and the absence of the SMSI. Instead, CO2 splitting to CO, both in the plasma gas phase and on the surface of ZnO, is believed to make an important contribution to the conversion of CO2 in the Plasma + ZnO system

No relationship between 2',3'-cyclic nucleotide 3'-phosphodiesterase and schizophrenia in the Chinese Han population: an expression study and meta-analysis

<p>Abstract</p> <p>Background</p> <p>2',3'-Cyclic nucleotide 3'-phosphodiesterase (<it>CNP</it>), one of the promising candidate genes for schizophrenia, plays a key part in the oligodendrocyte function and in myelination. The present study aims to investigate the relationship between <it>CNP </it>and schizophrenia in the Chinese population and the effect of different factors on the expression level of <it>CNP </it>in schizophrenia.</p> <p>Methods</p> <p>Five <it>CNP </it>single nucleotide polymorphisms (SNPs) were investigated in a Chinese Han schizophrenia case-control sample set (n = 180) using direct sequencing. The results were included in the following meta-analysis. Quantitative real-time polymerase chain reaction (PCR) was conducted to examine <it>CNP </it>expression levels in peripheral blood lymphocytes.</p> <p>Results</p> <p>Factors including gender, genotype, sub-diagnosis and antipsychotics-treatment were found not to contribute to the expression regulation of the <it>CNP </it>gene in schizophrenia. Our meta-analysis produced similar negative results.</p> <p>Conclusion</p> <p>The results suggest that the <it>CNP </it>gene may not be involved in the etiology and pathology of schizophrenia in the Chinese population.</p

Locally advanced rectal cancer with dMMR/MSI-H may be excused from surgery after neoadjuvant anti-PD-1 monotherapy: a multiple-center, cohort study

ObjectiveExamine patients with locally advanced rectal cancer (LARC) with deficient mismatch repair (dMMR) or microsatellite instability-high (MSI-H) who received neoadjuvant immunotherapy (nIT), and compare the outcomes of those who chose a watch-and-wait (WW) approach after achieving clinical complete response (cCR) or near-cCR with those who underwent surgery and were confirmed as pathological complete response (pCR).MethodsLARC patients with dMMR/MSI-H who received nIT were retrospectively examined. The endpoints were 2-year overall survival (OS), 2-year disease-free survival (DFS), local recurrence (LR), and distant metastasis (DM). The efficacy of programmed cell death protein-1 (PD-1) inhibitor, immune-related adverse events (irAEs), surgery-related adverse events (srAEs), and enterostomy were also recorded.ResultsTwenty patients who received a PD-1 inhibitor as initial nIT were examined. Eighteen patients (90%) achieved complete response (CR) after a median of 7 nIT cycles, including 11 with pCR after surgery (pCR group), and 7 chose a WW strategy after evaluation as cCR or near-cCR (WW group). Both groups had median follow-up times of 25.0 months. Neither group had a case of LR or DM, and the 2-year DFS and OS in each group was 100%. The two groups had similar incidences of irAEs (P=0.627). In the pCR group, however, 2 patients (18.2%) had permanent colostomy, 3 (27.3%) had temporary ileostomy, and 2 (18.2%) had srAEs.ConclusionNeoadjuvant PD-1 blockade had high efficacy and led to a high rate of CR in LARC patients with dMMR/MSI-H. A WW strategy appears to be a safe and reliable option for these patients who achieve cCR or near-cCR after nIT

Effect of Surface Straw Incorporation Rate on Water–Salt Balance and Maize Yield in Soil Subject to Secondary Salinization with Brackish Water Irrigation

Secondary salinization induced by brackish water irrigation has forced agricultural development to increasingly rely on soil management. A two-year field experiment was conducted to explore the effects of different straw incorporation rates (SIRs) within 0 to 20 cm topsoil on the soil water&ndash;salt balance, maize yield production, and water use efficiency (WUE) under brackish water irrigation in a naturally non-saline area. Air-dried wheat straw was applied at the rates of 0, 4.5, 9.0, 13.5, and 18.0 t ha&minus;1 (R0&ndash;R4) and two salinity levels of irrigation water with the salt content of 1.92 dS m&minus;1 (SL) and 3.20 dS m&minus;1 (SH) were applied for simulating the scenarios of secondary salinization. Results demonstrated that straw incorporation markedly increased the soil water content during two growing seasons, and SIR was directly correlated to the deep percolation, but inversely correlated to the soil water depletion, under both the SL and SH condition. Meanwhile, straw incorporation led to the increase in salt content within the straw incorporation zone, but the total mass of salt deposited in the 0&ndash;100 cm soil profile was comparatively reduced as SIR increased due to the increased deep percolation for salt leaching, and such relative alleviation was more pronounced under the SH condition. The significantly increased maize yield and its corresponding WUE were obtained in treatments with high SIR levels. Additionally, an exponential function was used to describe the trend of the yield-increasing rate as SIR increased, and the theoretical maximum of grain and biomass yield calculated from the fitting results were 6483 in 17,282 kg ha&minus;1 under SL, and 5440 and 14,501 kg ha&minus;1 under SH, respectively. Results in this study would be helpful in the adoption of straw incorporation and brackish water irrigation in ways that facilitate soil water availability and reduce the risk of soil salinization

Effect of Straw Biochar on Soil Properties and Wheat Production under Saline Water Irrigation

Use of saline water for irrigation is essential to mitigate increasing agricultural water demands in arid and semi-arid regions. The objective of this study is to address the potential of using straw biochar as a soil amendment to promote wheat production under saline water irrigation. A field experiment was conducted in a clay loam soil from eastern China during 2016/2017 and 2017/2018 winter wheat season. There were five treatments: freshwater irrigation (0.3 dS m&minus;1), saline water irrigation (10 dS m&minus;1), saline water irrigation (10 dS m&minus;1) combined with biochar of 10, 20, 30 t ha&minus;1. Saline water irrigation alone caused soil salinization and decreased wheat growth and yield. The incorporation of biochar decreased soil bulk density by 5.5%&ndash;11.6% and increased permeability by 35.4%&ndash;49.5%, and improved soil nutrient status. Biochar also reduced soil sodium adsorption ratio by 25.7%&ndash;32.6% under saline water irrigation. Furthermore, biochar alleviated salt stress by maintaining higher leaf relative water content and lower Na+/K+ ratio, and further enhanced photosynthesis and relieved leaf senescence during reproductive stages, leading to better grain formation. Compared to saline water irrigation alone, biochar application of 10 and 20 t ha&minus;1 significantly increased wheat grain yield by 8.6 and 8.4%, respectively. High dose of biochar might increase soil salinity and limit N availability. In the study, biochar amendment at 10 t ha&minus;1 would be a proper practice at least over two years to facilitate saline water irrigation for wheat production. Long-term studies are recommended to advance the understanding of the sustainable use of straw biochar

Assess Effectiveness of Salt Removal by a Subsurface Drainage with Bundled Crop Straws in Coastal Saline Soil Using HYDRUS-3D

The low permeability of soil and high investment of salt management pose great challenges for implementation of land reclamation in coastal areas. In this study, a temporary soil leaching system was tested in which bundled maize straw (straw drainage module, SDM) was operated as a subsurface drainage tube and diluted seawater was used for leaching. A preliminary field experiment was conducted in coastal soil-filled lysimeters to examine the system&#8217;s feasibility and a numerical model (HYDRUS-3D) based on field measured data was designed to simulate the entire leaching process. The simulation results showed that the soil water velocity and the non-uniformity of salt distribution were apparently enhanced in the region approaching the drain outlet. The mass balance information indicated that the amount of water drained with SDM accounts for 37.9&#8722;66.0% of the total amount of leaching water, and the mass of salt removal was about 1.7 times that of the salt input from the diluted seawater. Additional simulations were conducted to explore the impacts of the design parameters, including leaching amount, the salinity of leaching water, and the number of leaching events on the desalination performance of the leaching system. Such simulations showed that the salt removal efficiency and soil desalination rate both were negatively related to the seawater mixture rate but were positively associated with the amount of leaching water. Increasing the leaching times, the salt removal efficiency was gradually decreased in all treatments, but the soil desalination rate was decreased only in the treatments leached with less diluted seawater. Our results confirmed the feasibility of the SDM leaching system in soil desalination and lay a good foundation for this system application in initial reclamation of saline coastal land

An Automatic Concrete Crack-Detection Method Fusing Point Clouds and Images Based on Improved Otsu’s Algorithm

Cracks are one of the main distresses that occur on concrete surfaces. Traditional methods for detecting cracks based on two-dimensional (2D) images can be hampered by stains, shadows, and other artifacts, while various three-dimensional (3D) crack-detection techniques, using point clouds, are less affected in this regard but are limited by the measurement accuracy of the 3D laser scanner. In this study, we propose an automatic crack-detection method that fuses 3D point clouds and 2D images based on an improved Otsu algorithm, which consists of the following four major procedures. First, a high-precision registration of a depth image projected from 3D point clouds and 2D images is performed. Second, pixel-level image fusion is performed, which fuses the depth and gray information. Third, a rough crack image is obtained from the fusion image using the improved Otsu method. Finally, the connected domain labeling and morphological methods are used to finely extract the cracks. Experimentally, the proposed method was tested at multiple scales and with various types of concrete crack. The results demonstrate that the proposed method can achieve an average precision of 89.0%, recall of 84.8%, and F1 score of 86.7%, performing significantly better than the single image (average F1 score of 67.6%) and single point cloud (average F1 score of 76.0%) methods. Accordingly, the proposed method has high detection accuracy and universality, indicating its wide potential application as an automatic method for concrete-crack detection