生產改質幾丁質之方法及應用該改質幾丁質純化幾丁質酵素之方法

本發明係一種純化幾丁質酵素之方法，其係包括提供經發酵改質的幾丁質，使發酵改質的幾丁質與幾丁質酵素接觸，使幾丁質酵素吸附於發酵改質的幾丁質形成複合物，及自複合物中分離幾丁質酵素。本發明另相關於一種生產發酵改質之幾丁質的方法，其係包括提供可生產幾丁質酵素的微生物與幾丁質進行發酵，及自發酵液中分離幾丁質。本發明另相關於新穎微生物類芽孢桿菌(Paenibacillus sp.)

Effect of film-forming polymers on control of lily leaf blight caused by Botrytis elliptica

The effectiveness of film-forming polyelectrolytes for the control of lily leaf blight caused by Botrytis elliptica was evaluated using laboratory a leaf disk assay, greenhouse tests and field trials. Among the six polyelectrolytes, 400 ppm FO4240SH, FO4490SH and FO4550SH reduced the disease severity of lily leaf blight in leaf-disk tests. Both FO4240SH and FO4490SH also suppressed sporulation of the pathogen on leaf disks. In greenhouse tests, the number and size of lesions on leaves of Lilium oriental hybrid cv. Star Gazer were markedly reduced by FO4490SH and FO4550SH. Field trials showed that the effectiveness of FO4490SH was similar or better than that of procymidone on the reduction of lily leaf blight disease severity. The polymers had no harmful effects on the lily plants. The cationic polyelectrolytes FO4240SH, FO4490SH and FO4550SH reduced the percentage of conidial germination, inhibited germ-tube growth, and also suppressed the esterase production by germ tubes of B. elliptica. All the above evidence indicates that the disease control achieved with polyelectrolytes is due, at least in part, to the reduction of esterase secretion by B. elliptica

生產治黴色基素之方法

本發明係一種生產治黴色基素之方法，其係提供一培養基，包括約0.01至2.0%脫脂黃豆粉、約0.01至2.0%葡萄糖及約0.01至1.0%碳酸鈣，將鏈黴菌(Streptomyces padanus PMS－702)培養於該培養基質中，設定培養條件為轉速約100至400rpm、通氣量約0.5至1.5vvm及培養基質呈弱酸性，並於培養過程中添加油脂或脂肪酸或其混合物於培養基質中，進行大量液態培養，以獲取治黴色基素

New production method of convex microlens arrays for integrated fluorescence microfluidic detection systems

A new method for producing microlens array with large sag heights is proposed for integrated fluorescence microfluidic detection systems. Three steps in this production technique are included for concave microlens array formations to be integrated into microfluidic systems. First, using the photoresist SU-8 to produce hexagonal microchannel array is required. Second, UV curable glue is injected into the hexagonal microchannel array. Third, the surplus glue is rotated by a spinner at high velocity and exposed to a UV lamp to harden the glue. The micro concave lens molds are then finished and ready to produce convex microlens in poly methsiloxane (PDMS) material. This convex microlens in PDMS can be used for detecting fluorescence in microfluidic channels because a convex microlens plays the light convergence role for optical fiber detection

Application of antagonistic rhizobacteria for control of Fusarium seedling blight and basal rot of lily

Three antagonistic bacteria, Streptomyces misionensis strain PMS101, Bacillus thermoglucosidasius strain PMB207, and S. sioyaensis strain PMS502, were tested for sensitivity to the foliar fungicide Sporgon (a.i. 50% prochloraz-Mn complex) and for efficacy in controlling Fusarium diseases of lily. Results showed that the growth of all three antagonistic strains of bacteria was completely suppressed by Sporgon at a concentration of 500 mu g/mL, but B. thermoglucosidasius strain PMB207 and S. misionensis strain PMS101 were unaffected at concentrations of 100 mu g/mL or lower. A large-scale trial in an automated and environment-controlled commercial greenhouse showed that treatment of scale bulblets of lily with Sporgon (100 mu g /mL) and B. thermoglucosidasius strain PMB207 (1-1.2 x 10(7) cfu/mL) or 100 mu g/mL Sporgon and S. misionensis strain PMS101 (1-1.4 x 10(7) cfu/mL) resulted in a significant reduction (P 0.05). Results of the greenhouse and field experiments showed that treatment of scale bulblets or one-year-old bulbs of lily with B. thermoglucosidasius strain PMB207 (1-1.2 x 10(8) cfu/mL) or S. misionensis strain PMS101 (1-1.4 x 10(8) cfu/mL) without Sporgon was also effective in the control of basal rot caused by F. oxysporum f. sp. lilii. These studies reveal that B. thermoglucosidasius strain PMB207 and S. misionensis strain PMS101 are biocontrol agents which have potential for use in the commercial production of lily bulbs, as they can be used alone or in combination with the fungicide Sporgon at low concentration (< 100 mu g/mL)