Study on constructed AtPCS1 plant expression vector and transformation of Medicago sativa

扩增拟南芥(ArAbIdOPSIS THAlIAnA)螯合肽合成酶(ATPCS1)全长基因;构建ATPCS1植物表达载体PbⅠ121-ATPCS1,进一步转化农杆菌EHA105;利用转化的农杆菌EHA105侵染甘农一号苜蓿(MEdICAgO SATIVA)叶片,经过80--100 d的筛选与培养,获得57株再生转基因植株。随机抽取其中9株进行PCr检测,其中6株为阳性。初步结果表明,ATPCS1基因已整合到苜蓿基因组中。The full length of AtPCS1 gene was amplified from Arabidopsis thaliana(ecotype Columbia),and AtPCS1 plant expression vector pBI121-AtPCS1 was constructed.Furthermore,the expression vector was transferred into Agrobacterium EHA105,and AtPCS1 was transferred into alfalfa(Medicago sativa) Gannong No.1 by leaf infection method.Fifty-seven transgenic alfalfa plants have been obtained after a period of 80-100 d after transformation.Six in nine random chosen plants were positive transgenic plants identified by AtPCS1-specific PCR.Results from this study showed that AtPCS1 has been transferred into genome of alfalfa.甘肃省教育厅研究生导师基金(0703-11);兰州理工大学博士基金(SB08200602