Adaptive-Adaptive Sidelobe Cancellation Strategy for Two-dimensional Phased Arrays

传统旁瓣对消方法采用单元天线作为辅助通道,系统对消输出的信噪比较低,且合成阵列存在较高的副瓣抬升隐患。针对二维稀疏阵列的应用特点,提出一种新的自适应—自适应旁瓣对消策略。该方法取代了传统方法中使用的单元天线以及数字加权方法,使用小型阵列作为辅助通道和射频加权,并借助辅助通道对干扰方向进行估计,根据估计得到的干扰方向信息对辅助子阵列内部的射频加权系数进行调整,使得辅助通道的波束最大可能地对准干扰方向。最后利用恒增益对消技术,实现主阵列中的旁瓣对消。仿真实验结果表明了该方法的有效性和优势。The traditional adaptive sidelobe cancellation method uses single antenna as assistant channel,so the signal to noise ratio( SNR) of system output is small,and it is possible to uplift the sidelobe in synthetic array. In view of application feature of two-dimensional sparse array,a new method named adaptive-adaptive sidelobe cancellation is proposed in this paper. The new method replaces the single antenna with small synthetic arrays as assistant channel and replaces digital weighting method with RF weighting method,and it estimates the direction of the jamming with assistant channel. According to the information about the direction,the new method adjusts the RF weight of assistant channel,and makes the beam of assistant channel align the jamming directions. The sidelobe cancellation method with constant gain is used to perform the sidelobe cancellation. The simulation results prove the effectiveness and advantages of the proposed method.国家自然科学基金资助项目(61301009);; 福建省自然科学基金计划资助项目(2013J01252);; 高等学校博士学科点专项科研基金资助项目(20120121120027

SERS Study of Apo-Metallothionein Adsorbed on Roughened Gold Surfaces

利用表面增强拉曼光谱(SERS)技术研究了金属硫蛋白(MT)与脱金属硫蛋白(apo-MT)在粗糙金表面的吸附行为。结果表明:MT通过氨基与金作用;apo-MT与金的作用位点受制备方法影响:在溶液中预制的apo-MT通过半胱氨酸残基的巯基与金结合,在金表面制备的apo-MT通过氨基和巯基与金结合。SERS更适合于研究MT的结构特征。The adsorption of metallothionein and apo-metallothionein on roughened Au surfaces was studied by SERS technique.The results indicate that MT is adsorbed via-NH2 group on roughened gold surfaces.The adsorption site of apo-MT on Au is determined by the preparation method of apo-MT.The apo-MT prepared in solution is adsorbed via the-SH group on Au whereas that prepared on the Au surface is adsorbed via-SH and-NH2.国家自然科学基金(20503040,20773165);; 973计划(2007CB935603);; 厦门大学固体表面物理化学国家重点实验室开放课题项目资

自杀基因治疗恶性肿瘤的研究现状及展望

肿瘤的自杀基因疗法是近年来肿瘤基因治疗的研究热点,是一种具有潜在临床应用前景的新的肿瘤基因治疗策略。本文就近年来自杀基因疗法在肿瘤治疗中取得的研究进展,分别从作用机制、自杀基因系统、旁观者效应、自杀基因的转导以及联合基因治疗等几个方面进行综述

Inhibitory effect of NRP-1mAb on the growth of gastric cancer cell BGC-823

目的观察自主研发的抗NRP-1b1/b2IgG单克隆抗体（NRP-1mAb）对胃癌BGC-823细胞生长的影响,并初步探讨可能的作用机制。方法实验室制备NRP-1mAb,采用SDS-PAGE检测纯度。采用0、25、100、200、400μg/ml NRP-1mAb培养胃癌BGC-823细胞,光学显微镜下观察细胞形态学变化;采用MTT法、集落形成实验、流式细胞术观察胃癌细胞BGC-823的增殖、集落形成和凋亡的情况;Western blotting法检测NRP-1mAb作用后Akt、p38、ERK、JNK信号蛋白磷酸化情况。结果SDS-PAGE检测显示,NRP-1mAb的纯度在95%以上。光学显微镜观察显示,NRP-1mAb作用后,BGC-823细胞形态呈凋亡改变。MTT实验显示,NRP-1mAb抑制BGC-823细胞的增殖作用呈浓度和时间依赖性（P〈0.01）。集落形成实验显示,不同浓度NRP-1mAb均能显著抑制BGC-823细胞的集落形成,其抑制率呈浓度依赖性（P〈0.01）。流式细胞术检测显示,不同浓度NRP-1mAb均明显促进BGC-823细胞凋亡,且大部分集中在早期凋亡。Western blotting检测显示,BGC-823细胞的Akt磷酸化受到抑制,ERK、p38、JNK的磷酸化水平无明显变化。结论NRP-1mAb能抑制胃癌细胞BGC-823的生长、促进凋亡,可能与抑制Akt磷酸化有关。Objective To observe the effect of NRP-1b1/b2 IgG monoclonal antibody（ NRP-1） on the growth of gastric cancer cell BGC-823,and to explore the possible mechanism of the antibody. Methods NRP-1mAb was prepared in laboratory,and the purity of antibody was detected by SDS-PAGE. Gastric cancer BGC-823 cells were cultured by 0,25,100,200,400 μg/ml NRP-1 mAb. The morphological changes of BGC-823 cells were observed by microscope. The proliferation,colony formation and apoptosis of gastric cancer BGC-823 cells were observed by MTT assay,colony forming assay and flow cytometry. The phosphorylation of related signal proteins was detected by Western blotting analysis. Results SDS-PAGE test showed that the purity of NRP-1mAb was above95%. Microscopy showed apoptotic changes of BGC-823 cells treated by NRP-1mAb. MTT assay showed that NRP-1mAb could inhibit the proliferation of BGC-823 cells in a time and dose dependent manner（ P〈0. 01）. Colony forming assay showed that different doses of NRP-1mAb could inhibit the colony formation of BGC-823 cells in a dose dependent manner（ P〈0. 01）. Flow cytometry showed that different doses of NRP-1mAb could promote the apoptosis of BGC-823 cells mainly at early apoptosis stage. It was found that the level of Akt phosphorylation was decreased after treated by NRP-1mAb,and there was no significant phosphorylation of ERK,p38 and JNK protein. Conclusion NRP-1mAb can inhibit the growth of gastric cancer cell BGC-823 and promote apoptosis,which may be related to the inhibition of Akt phosphorylation.厦门市科技计划创新资助项目（3502z20134026,3502z20144034

Inhibitory effect of anti-human NRP-1 monoclonal antibody on hepatocellular carcinoma cell line HepG2 and its mechanism in vitro

目的研究抗人神经纤毛蛋白-1(Neuropilin1,NRP-1)单克隆抗体对肝癌Hep G2细胞的生长抑制作用及其机制。方法小鼠腹水法制备抗NRP-1单克隆抗体(NRP-1 m Ab)并用r Protein A亲和柱纯化抗体,间接ELISA检测抗体的滴度水平。Western blot检测NRP-1 m Ab对Hep G2细胞的特异性,细胞免疫荧光和流式细胞术检测NRP-1蛋白在肝癌细胞株Hep G2上的表达,MTT法检测NRP-1 m Ab对Hep G2的生长抑制作用,Western blot检测ERK1/2、P-ERK1/2、Akt、P-Akt蛋白的表达水平。结果 SDS-PAGE和间接ELISA检测纯化的NRP-1 m Ab纯度为95%以上,效价为1×10~(-6);Western blot检测结果显示NRP-1 m Ab可与Hep G2细胞膜上的NRP-1蛋白特异性结合。细胞免疫荧光染色结果显示NRP-1定位于Hep G2细胞膜,流式细胞术结果显示NRP-1蛋白在Hep G2细胞上表达水平较高;MTT法检测结果显示NRP-1 m Ab对Hep G2细胞有生长抑制作用。Western blot检测到在不同浓度NRP-1 m Ab作用下,Hep G2细胞裂解液P-ERK1/2、P-Akt蛋白的条带信号逐渐减弱。结论纯化的NRP-1m Ab能抑制Hep G2细胞的生长,其抑制作用是通过EGF和HGF信号通路实现的。The aim of the experimental is to investigate the inhibitory effect of anti-human nerve cilia protein1(Neuropilin-1,NRP-1) monoclonal antibody(NRP-1 mAb) on hepatocellular carcinoma cell line HepG2 and its mechanism in vitro.Anti-human NRP-1 monoclonal antibody(NRP-1 mAb) was prepared from mouse ascites and purified by rProteinA affinity column assay.The titer of antibody was determined using indirect ELISA assay;the characteristic of NRP-1 mAb binding to NRP-1 was determined using Western blotting;the expression of NRP-1protein in hepatocellular carcinoma cell line HepG2 was determined using immunofluorescence assay and flow cytometry assay.Growth inhibition of HepG2 cells treated with different concentrations of NRP-1 mAb was determined using MTT assay,while Western blotting was used to detect the expression levels of ERK1/2,P-ERK1/2,Akt and P-Akt proteins.The results of SDS-PAGE and indirect ELISA showed that the purity of purified NRP-1mAb was more than 95%and the titer was 1×10~(-6).Western blotting analysis suggested that NRP-1 mAb could bind specifically to NRP-1 on HepG2 cell;immunofluorescence staining showed that NRP-1 was located in the membrane of HepG2 cells.Flow cytometry analysis showed that the expression level of NRP-1 on HepG2 cell was relatively high.Western blotting analysis suggested that P-ERK1/2 and P-Akt expression levels were down-regulated after having incubated HepG2 cells with different concentrations of NRP-1 mAb.In conclusion,NRP-1 mAb could inhibit the growth of HepG2 cells(P<0.05),and its inhibitory effect is achieved by reducing the P-ERK1/2 and P-Akt expression.南京军区医学科技创新项目(12MA061,15MS104

Study on extraction technology of volatile oil from cortex phellodendri by supercritical carbon dioxide

使用超临界二氧化碳技术对经过超声-微波处理过的黄柏中的挥发油进行萃取,并对萃取工艺进行响应面优化。在单因素预实验的基础上,以萃取压力、萃取温度、萃取时间为响应因素,黄柏挥发油的萃取量为响应值,根据中心组合(bOX-bEHnkEn)实验设计原理,采用三因素三水平的响应面分析法,确定各工艺条件对萃取量的影响,并用扫描电子显微镜(SEM)对萃取前、未超声-微波处理超临界萃取后及超声-微波处理超临界萃取后的黄柏进行比较观察,对萃取效果进行了微观解释。结果表明,经过超声-微波处理过的黄柏中的挥发油超临界二氧化碳萃取最佳工艺条件为:萃取压力为34MPA,萃取温度为41℃,萃取时间为66MIn,萃取率达6.03%。The supercritical carbon dioxide extraction of volatile oil from processed cortex phellodendri was optimized by response surface methodology(RSM).According to the principle of Box-Behnken central composite design,extraction pressure,extraction temperature,extraction time were chosen as response factors,extraction mass was chosen as response value,and a three-factor and three level central composite design was adopted to determine the influence of various technological conditions.Using SEM observed the cortex phellodendri before and after extraction,and explained the extraction effects from microcosmic aspect.The results showed that the optimum extraction conditions were as follows:extraction pressure 34MPa,extraction temperature 41℃,extraction time 66min,extraction yield was 6.03%.湖南省科技厅重大专项(2008FJ1007);2010年吉首大学大学生研究性学习与创新性实验计划项目(JSU-CX-2010-49

Designable ultra-smooth ultra-thin solid-electrolyte interphases of three alkali metal anodes

该研究是在毛秉伟教授和董全峰教授共同指导下，由2014级博士生谷宇、2016级博士生王卫伟、2013级博士生李艺娟等同学通力合作的成果。泉州师范学院吴启辉教授和上海应用物理研究所樊春海研究员分别在X射线光电子能谱表征和同步辐射表征中提供了大力支持。化学化工学院郑明森副教授、颜佳伟教授、吴德印教授、iChEM研究员丁松园博士在实验和理论计算方面给予了大力帮助；田中群教授和郑南峰教授等对此工作提出了宝贵意见。 金属锂是下一代高能量密度二次电池最理想的负极材料之一。在以金属锂作为负极的电池中，锂电沉积过程中枝晶的生长及溶解过程的死锂现象是制约电池库仑效率、稳定性和安全性的重要因素，而金属锂表面的微观平整性及固态电解质界面（SEI）膜的组成和结构及由其所决定的电学和力学性质对锂电沉积和溶解行为有重要影响。 课题组所发展的电化学调控方法可进一步拓展到钠、钾碱金属负极上，形成大范围原子平整的钠、钾表面和超薄超光滑的SEI膜。特别是金属钠平面电极可在2 mA cm–2（1 mAh cm–2）100% Na DOD下稳定循环550周以上且库伦效率近100%。这一基于电化学调控的碱金属表面抛光和SEI膜构筑的方法及多尺度联合表征，为解决碱金属负极问题提供了新的思路，也为开展表面科学基础研究提供高质量的碱金属表面。【Abstract】Dendrite growth of alkali metal anodes limited their lifetime for charge/discharge cycling. Here, we report near-perfect anodes of lithium, sodium, and potassium metals achieved by electrochemical polishing, which removes microscopic defects and creates ultra-smooth ultra-thin solid-electrolyte interphase layers at metal surfaces for providing a homogeneous environment. Precise characterizations by AFM force probing with corroborative in-depth XPS profile analysis reveal that the ultra-smooth ultra-thin solid-electrolyte interphase can be designed to have alternating inorganic-rich and organic-rich/mixed multi-layered structure, which offers mechanical property of coupled rigidity and elasticity. The polished metal anodes exhibit significantly enhanced cycling stability, specifically the lithium anodes can cycle for over 200 times at a real current density of 2 mA cm-2 with 100% depth of discharge. Our work illustrates that an ultra-smooth ultra-thin solid-electrolyte interphase may be robust enough to suppress dendrite growth and thus serve as an initial layer for further improved protection of alkali metal anodes.This work was supported by the MOST projects (2015CB251102, 2012CB932902) and the NSFC projects (U1305246, 21621091, 21473147, 21533006, 21673193). 研究工作得到科技部973计划（项目批准号：2015CB251102、2012CB932902），国家自然科学基金（项目批准号：U1305246、21621091、21473147、21533006、21673193）等项目的资助