Application of Raman Spectroscopy for Analysis in Radiosensitivity of Total Protein of Nasopharygeal Carcinoma Cells

本文以鼻咽癌细胞株CnE2为放射敏感性的研究对象,经不同剂量X射线照射及不同时间培养后分别提取总蛋白,用共聚焦显微拉曼光谱仪检测其拉曼光谱。统计分析表明:被测样品的拉曼光谱中观察到一些可以归属于蛋白质物质的较为明显的基团频率振动峰;不同剂量的X射线照射后,总蛋白质的平均拉曼光谱与对照组谱形基本一致,但与对照组间的光谱存在着对应峰信号强度的不同。实验提示:照射后谱峰强度的增大或减小,提示着相关物质含量有所改变。分析照射后癌细胞总蛋白拉曼光谱的变化情况,结合数学统计方法,以探寻放射敏感性的特征拉曼标志,可以作为研究肿瘤放射敏感性的手段之一。To study the effects of total protein of nasopharyngeal carcinoma cell line CNE2 treated by different doses of X-ray irradiation.The total protein is extracted from the cell line and detected by confocal micro-Raman spectroscopy.Both t test and support vector machine are employed via SPSS and LIBSVM software packages for statistical analysis of Raman spectrum.The results show that several Raman bands assigned to total protein can be detected; and the X-ray irradiation have various effects on different components in total protein,and related to the radiation dose.It was indicated that in spite of no significant diversification on structures of protein,the content may be varied during irradiation.国家自然科学基金项目(11104030); 福建省自然科学基金项目(2011J01153