Root-inhibiting Activity of Hydrolized Peptides of Corn Gluten Meal on Smoothscale Kyllinga(Kyllinga brevitolia var.leiolepis)

以木瓜蛋白酶、中性蛋白酶为水解酶,以玉米蛋白粉为底物,制备出不同酶解时间条件下的玉米蛋白水解多肽。用培养皿生物分析法检测不同玉米蛋白水解多肽在不同浓度(0.5 mg/mL、1 mg/mL、2 mg/mL、5 mg/mL)条件下对光磷水蜈蚣(Kyllinga brevitoliavar.leiolepis)种子生根抑制活性。结果表明:①两种蛋白酶不同水解时间制备的玉米蛋白粉水解多肽对光鳞水蜈蚣种子萌发生根均有抑制作用,且随着浓度增高生根抑制活性增强;②木瓜蛋白酶在水解3 h和6 h制得玉米多肽的生根抑制活性明显优于水解9 h和12 h制得的玉米多肽,而中性蛋白酶在不同水解时间水解玉米蛋白粉所得的水解多肽对光磷水蜈蚣的抑制活性无显著性差异;③木瓜蛋白酶制备的玉米多肽比中性蛋白酶制备的玉米多肽生根抑制活性强,并达到极显著性差异水平(P<0.01)。Corn gluten peptides were prepared by two proteases in different hydrolysis times and their root-inhibiting activity on Kyllinga brevitolia var.leiolepis was conducted in petri dish bioassays at rate of 0,0.5 mg/mL,1 mg/mL,2 mg/mL,5 mg/mL.The result showed: ①Corn gluten peptides prepared by two proteases have root-inhibiting activity on Kyllinga brevitolia var.leiolepis,and their bioactivity becomes greater with the increasing concentrations.② Inhibitory activities of peptides changed with hydrolyzing time using papain to hydrolyze CGM and corn gluten peptides prepared at hydrolysis time of 3 hours and 6 hours exhibited more inhibitory activity than that of 9h and 12h.On the contrary,no difference was found for the peptides prepared by neutrase in different hydrolysis time.③ Compared with papain,peptides prepared by neutrase is less inhibitory active and the ANOVA analysis shows highly significantly difference.Therefore,as a hydrolysase,papain can be taken priority to prepare the corn gluten peptides.广东省科技发展项目(03KJ6062

养正消积胶囊辅助GP方案治疗晚期非小细胞肺癌临床研究

目的观察养正消积胶囊辅助GP方案治疗晚期非小细胞肺癌(NSCLC)的临床疗效。方法采用随机数字表法将89例晚期NSCLC患者分为对照组44例及观察组45例。2组均采用GP方案;观察组并予养正消积胶囊,每次4粒,每日3次,口服。2组均21d为1个周期,连续治疗2个周期,随访1年。观察2组治疗前后Ki67、Bax、Bcl-2蛋白表达及肿瘤标志物[血管内皮生长因子(VEGF)、骨桥蛋白(OPN)、癌胚抗原(CEA)、糖类抗原199(CA199)]、中医症状评分、生活质量评分,比较2组客观缓解率(ORR)、疾病控制率(DCR)、无进展生存时间(PFS),监测不良反应。结果观察组ORR、DCR明显优于对照组(P<0.05)。与本组治疗前比较,观察组治疗后、随访1年VEGF、OPN、CEA、CA199水平明显降低(P<0.05);对照组治疗后上述指标明显降低(P<0.05),随访1年CEA水平明显降低(P<0.05)。与本组治疗前比较,2组治疗后Ki67、Bcl-2蛋白表达明显降低,Bax蛋白表达明显升高(P<0.05);2组治疗后比较,观察组Ki67、Bcl-2蛋白表达低于对照组(P<0.05),Bax蛋白表达高于对照组(P<0.05)。与本组治疗前比较,2组治疗后中医症状评分(神疲乏力、胃纳少馨、腰膝无力、夜尿频多、头晕目眩)、生活质量评分(躯体功能、认知功能、社会功能、情绪功能)明显改善(P<0.05);2组治疗后比较,观察组上述评分改善明显优于对照组(P<0.05)。观察组PFS明显长于对照组(P<0.05),不良反应明显低于对照组(P<0.05)。结论养正消积胶囊辅助GP方案治疗晚期NSCLC可有效降低患者肿瘤标志物水平,改善生存质量,减少吉西他滨和顺铂的不良反应,提高疗效。福建省卫生厅中医药科研专项课题(wst201210

An Extension of the Convolution Equivalent Class and Their Some Properties

给出卷积等价分布簇S(γ)的一种扩张,并研究它们的性质,发现新簇也具有卷积的封闭性等性质.We give an extension of the convolution equivalent class S(γ)(see Definition D),and study their some properties,we find that the new class also has closure of convolution.伊犁师范学院重点学科开放课题(2012ZDXK04