Effects of curcumin on anti-proliferation and expression of apoptosis interrelated gene of Human Osteosarcoma MG-63 Cells

目的:探讨姜黄素对人成骨肉瘤Mg-63细胞增殖抑制及凋亡相关基因表达的影响。方法:用姜黄素处理Mg-63细胞,细胞计数法检测细胞增殖抑制的效果;荧光染色观察细胞的凋亡;流式细胞仪(fCM)进行细胞周期时相分析;免疫细胞化学法和WESTErn blOTTIng免疫法检测细胞凋亡相关基因的表达水平。结果:随着姜黄素浓度的增加及其作用时间延长,对细胞的增殖抑制作用增强,最高抑制率可达89.07%;光镜下可观察到细胞发生染色质浓缩,细胞核凝聚和碎裂等典型的凋亡形态学改变;fCM检测结果显示细胞经姜黄素处理后出现明显的凋亡峰;免疫反应结果显示,凋亡相关基因bCl-2和P53表达水平降低,而bAX和fAS表达水平升高。结论:姜黄素能显著抑制Mg-63细胞增殖并可有效诱导其凋亡,其作用机制可能与bCl-2和bAX二者的比值发生变化从而接受了凋亡刺激信号有关。Objective: To investigate the regulatory effect of curcumin on proliferation inhibition and apoptosis in human osterosarcoma MG-63 cells.Methods: MG-63 cells were treated by curcumin, and the inhibitory effect on cell proliferation was assessed by cell count.The apoptosis was inspected by fluorescent staining.The cell cycle analysis was detected by FCM.The expression level of genes related with apoptosis was observed by immunohistochemistry and western blotting immunoreaction.Results: The results showed that the cells growth was inhibited with the increasing of the concentration of curcumin and the elongation of the treated time,and the highest inhibition rate was 89.07%.Parts of cells presented the typical morphological changes of apoptosis: chromatin concerntrated and agglutinatied, cell nucleus shrinked and cataclased under the microscope.The results of FCM analysis showed that curcumin could in- duce cells into apoptosis, which was proved by the emergence of apoptotic peak.The results of immunoreaction suggested that the expression level of bcl-2 and P53 were down-regulated and the expression level of bax and Fas were upregulated.Conclusions: Curcumin not only inhibited cell proliferation but also induced apoptosis of MG-63 cells, which may be related to the ratio of the expression level of bcl-2 and bax as well as reception of the signal of stimulating apoptosis.国家自然科学基金(30871241

Curcumin-induced Apoptosis Affects the Expression and Localization of hnRNP A2/B1 in Human Osteosarcoma MG-63 Cells

姜黄素(CurCuMIn)诱导处理的人成骨肉瘤Mg-63细胞,在光镜和电镜观察细胞凋亡的基础上,对HnrnP A2/b1在核基质中存在、分布及其与凋亡相关基因产物在Mg-63细胞中的共定位关系进行了研究.经姜黄素处理后,细胞出现染色质凝聚、细胞核固缩、凋亡小体等典型的细胞凋亡形态特征;双向凝胶电泳和质谱鉴定结果显示,HnrnP A2/b1存在于Mg-63细胞核基质蛋白组分中,在姜黄素处理后细胞核基质蛋白中表达下调.蛋白质印迹杂交结果,证实HnrnP A2/b1在姜黄素处理前后的Mg-63细胞核基质蛋白中的存在及其表达下调变化.免疫荧光显微镜观察显示,HnrnP A2/b1定位于Mg-63细胞核基质纤维上,经姜黄素处理后出现分布位置与表达水平变化.激光扫描共聚焦显微镜的观察结果显示,HnrnP A2/b1在Mg-63细胞凋亡过程中与bAX、bCl-2、fAS和P53等基因产物具有共定位关系,且其共定位区域发生了变化.研究结果证实了HnrnP A2/b1定位于核基质纤维上,是一种核基质蛋白,在姜黄素诱导人成骨肉瘤Mg-63凋亡过程中的表达与分布变化及其与凋亡相关基因的关系显然对Mg-63细胞凋亡具有重要影响,这为深入揭示肿瘤细胞凋亡的机制提供了重要科学依据和深入探索的新方向.The morphology of human osteosarcoma MG-63 cells under light microscopy and transmission election microscopy was altered when treated with curcumin.The distribution of heterogeneous nuclear ribonucleoprotein (hnRNP)A2 /B1 in the nuclear matrix and its colocalization with the apoptosis-related gene products were examined.The results demonstrated that curcumin induced typical apoptotic responses,such as chromatin agglutination,nuclei condensation,and apoptotic body formation.Twodimentional PAGE and mass spectrum analyses showed that hnRNP A2 /B1 was complexed with the nuclear matrix.Confirmed by Western blotting,curcumin treatment resulted in a down-regulation of hnRNP A2 /B1 expression.An altered distribution of hnRNP A2 /B1 with the nuclear matrix was observed in immunofluorescence assays.Laser confocal microscopy showed that hnRNP A2 /B1 colocalized with apoptosis-related molecules,including Bax,Bcl-2,Fas and p53,with different patterns before and after curcumin treatments.Our study showed that expression level and the distribution of hnRNP A2 /B1,a nuclear matrix protein,were altered by curcumin treatments and mought be involved in the process of apoptosis in MG-63 cells.国家自然科学基金项目(No.30871241)---

集装箱海运收益管理舱位的定价模型

通过引入集装箱货流不平衡因子,系统地考察重箱和空箱运输,基于收益管理建立了考虑空箱调运的集装箱舱位最优定价模型,讨论了模型性质,并对模型进行数值分析,得出一系列有益的性质和管理策略。国家自然科学基金资助项目(70272022/70302014

基于SDT圆度误差模型下滑动轴承油膜特性的研究

建立了轴颈存在圆度误差的滑动轴承转子系统动力学模型，基于小位移旋量（Small displacement theory，SDT）理论导出了新的广义圆度误差方程，并在Swift-Stieber边界条件下对雷诺方程进行数值求解，着重分析了基于SDT表征的圆度误差旋量参数dx对滑动轴承油膜压力、承载能力、临界转速以及稳定性的影响。研究结果表明，圆度误差旋量参数会改变最大油膜压力，而且油膜压力会随着旋量参数的增大而增大；此外，轴颈圆度误差的存在会在一定程度上有利于滑动轴承转子系统的承载能力及稳定性，且随着圆度误差旋量参数的增大，其促进作用更加明显；其中，当偏心率ε<0.8时，轴颈圆度误差对系统承载能力的影响更加明显，当Sommerfeld数S>0.6时，轴颈圆度误差对稳定性的影响较小；同时，系统的临界转速也会随着旋量参数的增大而增大，特别是在偏心率ε>0.6时，增大现象更加明显

A Stochastic Model for Capacity Allocation of Container Shipping Two-dimensional Revenue Management

在充分考虑集装箱海运的二维属性,包括不同尺寸的集装箱类型和班轮的容量与重量双重约束的基础上,基于收益管理的思想建立了多班轮、多航段能力分配的随机模型。运用稳健优化方法将其转化为整数线性规划模型,并给出了实际应用中情景及其发生概率的获取方法,然后采用遗传算法进行求解。在算例中,得到了不同箱型的最优和满意的承运方案,研究结果表明了模型和算法的有效性。最后,通过数值分析和讨论得到3个有益的性质和管理策略。Based on revenue management,a stochastic model for multi-liner and multi-segment capacity allocation is proposed.This model adequately considers the two-dimensional characteristic of container shipping,which includes container types with different size and double constraints of volume and weight of a liner ship.It is transformed into a linear integer programming model by robust optimization method.The method is given to get the scenarios and the probability that each scenario occurs.The model is then solved by genetic algorithms.In numerical examples,the optimal and satisfactory allocation solutions of different container types are gained and the research results show the validity of the model and algorithm.Finally,through numerical analysis and discussion,three beneficial characters and management strategies are drawn.国家自然科学基金资助项目(70272022,70302014

Turbulent Mixing and Scale-up of Ejectors at High Schmidt Number

Turbulent Mixing and Scale-up of Ejectors at High Schmidt Numbe

CFD Simulation of Geometric Effects on Jet Reactors

CFD Simulation of Geometric Effects on Jet Reactor