ApoE基因多态性与动脉粥样硬化性脑梗死的相关性研究

目的分析ApoE基因与血脂和颈动脉粥样硬化程度的关系,进一步探讨ApoE基因与动脉粥样硬化性脑梗死的相关性。方法采用病例-对照研究的方法,收集厦门大学附属第一医院神经内科的95例缺血性脑卒中和65例非缺血性脑卒中住院患者。检测患者的ApoE基因型、血脂及颈动脉粥样硬化程度。结果与E2/E3比较,基因型E3/E3、E3/E4可能是缺血性脑卒中的保护因素;缺血性和非缺血性脑卒中患者在血浆TC、LDL-C、ApoB含量比较上均为E3/E4> E3/E3>E2/E3,且2组血浆LDL-C浓度在E3/E4与E2/E3、E3/E3比较中差异有统计学意义(P<0. 05); LDL-C、ApoB与2组颈动脉粥样硬化斑块程度有关,是其危险因素;与基因型E2/E3比较,E3/E4在有斑块组中较无斑块组比例高。结论基因型E3/E3、E3/E4可能是缺血性脑卒中的保护因素。基因型E3/E4可能是导致患者颈动脉粥样斑块形成的危险因素,基因型E3/E4通过影响患者的血浆LDL-C水平进一步导致动脉粥样硬化斑块的形成,但与动脉粥样硬化性脑梗死的相关性研究有待更大的样本量更进一步的研究

Three-Dimensional N-doped Graphene/Hemoglobin-Based Electrochemical Sensor for Detection of Acrylamide

本研究利用氮掺杂三维石墨烯/血红蛋白(3d n-rgO/Hb)修饰的玻碳电极,实现对食品中丙烯酰胺(AA)的电化学快速检测。以3d n-rgO作为玻碳电极上电子传递材料,利用其疏松多孔、吸附能力强及电子传输通道多等优势,增强了AA与Hb的相互作用,从而降低Hb的电化学信号,实现对AA的响应。实验采用示差脉冲伏安法(dPV)检测AA,所测得氧化还原峰电位为-350MV,峰电流值随AA浓度增大而逐渐减小。在AA浓度为1.4x10-12~1.4x10-4 MOl/l范围内,峰电流强度与AA浓度呈现良好的线性关系(r2=0.9799),检出限(S/n=3)为1.0x10-12 MOl/l。研究表明该方法制备的涂层厚度均一且不易脱落,能够实现对实际样品中AA的检测。Based on our previous research,three-dimensional N-doped graphene/hemoglobin(3D N-RGO/Hb) was prepared by hydrothermally assembled method.Benefiting from its excellent electrical conductivity,high surface area,biocompatibility and mechanical properties,3D N-RGO was used as support material of Hb,and a 3D N-RGO/Hb modified glassy carbon electrode(GCE)was then developed for the detection of acrylamide(AA).Differential pulse voltammogram(DPV)results showed that the 3D N-RGO/Hb modified GCE presented highly sensitive and stable sensing characteristics for AA detection.The analytical performance of the Nafion/Hb/N-RGO/GCE toward AA was evaluated in the linear response range from 1.4×10-12 to 1.4×10-4 mol/L,with a detection limit(S/N=3)of 1.0×10-12 mol/L.These results indicated that the proposed method was promising for the development of novel electrochemical sensing for AA detection.福建省科技重大专项专题项目(No.2011YZ0001-1

Study of Single-phase Three-level NPC Rectifier Based on Space Vector PWM

Working principle of single-phase three-level neutral-point-clamped (NPC) rectifier was discussed, and the relation between SVPWM and carrier-based PWM was established, thereby, balance control method based on SPWM for single phase three-level NPC rectifier was presented. Computer simulation and experiment results demonstrated that the proposed neutral-point voltage balance control method could solve the neutral-point voltage unbalance problem effectively even if the parameters of the two capacitors were different