通过锌催化区域选择性氧化反应实现3-烯-1-炔的1,4-官能团化

γ-羟基或烷氧基取代的α,β-不饱和羰基化合物广泛存在于众多天然产物和生物活性分子之中.本工作通过锌催化的区域选择性的氮氧化合物氧化炔烃反应,实现了3-烯-1-炔的4-烷氧化-1-氧化官能团..

通过锌催化区域选择性氧化反应实现3-烯-1-炔的1,4-官能团化

羟基或烷氧基取代的α,β-不饱和羰基化合物广泛存在于众多天然产物和生物活性分子之中.通过锌催化的区域选择性的氮氧化合物氧化炔烃反应,实现了3-烯-1-炔的4-烷氧化-1-氧化官能团化.该串联反应可以中等到良好的产率得到系列合成上很有用的γ-烷氧基取代的α,β-不饱和酰胺化合物.浙江省公益技术应用研究计划(No.LGG19B040001)台州市科技计划(No.1801gy21)国家自然科学基金(Nos.21572186,21622204)教育部创新团队资助项目~

冗余双臂构型血管造影机

本实用新型属于医疗器械技术领域，尤其涉及冗余双臂构型血管造影机。其可实现大范围内，不同角度、不同等中心距离的等中心检测。包括车体；其特征在于，所述车体内设置有床体，床体上方设置有机器人上臂，床体下方设置有机器人下臂；上臂端部与血管造影机的平板探测器相连，下臂端部与血管造影机的阳极球管相连；通过上、下臂的运动，实现大范围内、不同角度、不同等中心距离的等中心检测，为微创手术过程中提供高质量的造影图像

氯化镉、灭多威和硝基苯对日本青鳉(Oryzias latipes)行为抑制的生物应急监测

研究了氯化镉、灭多威和硝基苯对孵化以后7d左右日本青鳉(Oryzias latipes)行为毒性。结果表明,在不同污染物中,随暴露浓度的增加,第1尾日本青鳉产生行为抑制的时间逐渐缩短,并且半数行为抑制时间(time of half behavior inhibition,THBI,Y)随暴露浓度(X)呈现幂指数关系Y=aXb,其中20<a<28,-1.2<b<-0.8。实验结果表明基于日本青鳉THBI分析,能初步实现水体突发性污染事故污染水平的应急监测,从而为污染事故应急预案提供支持

Biological Emergency Monitoring on the Behavior Inhibition of Japanese Medaka(Oryzias latipes)Exposed to Cadmium Chloride,Methomyl and Nitrobenzene

研究了氯化镉、灭多威和硝基苯对孵化以后7d左右日本青鳉(Oryzias latipes)行为毒性。结果表明,在不同污染物中,随暴露浓度的增加,第1尾日本青鳉产生行为抑制的时间逐渐缩短,并且半数行为抑制时间(time ofhalf behavior inhibition,THBI,Y)随暴露浓度(X)呈现幂指数关系Y=aXb,其中20</a<28,-1.2<b<-0.8。实验结果表明基于日本青鳉thbi分析,能初步实现水体突发性污染事故污染水平的应急监测,从而为污染事故应急预案提供支持。

Composition and distribution of TCBS bacteria groups from sediments of Jiulong River estuary

通信作者。Tel /Fax: + 86-592-2195769; E-mail: heyangli@ 126. com 作者简介: 陈明霞( 1980 －) ,女,福建惠安人,博士,微生物专业,主要从事海洋微生物分子多样性研究。E-mail:chenmx1257@ 163.com[中文文摘]【目的】调查九龙江流域对厦门海域潜在的病原菌"污染",为相关侵染性病害的预防和控制提供有价值的资料。【方法】通过TCBS(Thiosulfate Citrate Bile Salts Sucrose)培养基从九龙江河口沉积物中分离到158株细菌,应用16S rRNA基因-RFLP(限制性酶切图谱多样性分析)及16S rRNA基因序列分析等方法对158株细菌进行分子鉴定。【结果】研究结果表明九龙江口沉积物中分布的TCBS菌群分别属于7个属,其中假单胞菌属(Pseudomonas)占28%,气单胞菌属(Aeromonas)占24%,假交替单胞菌属(Pseudoalteromonas)占19%,希瓦氏菌属(Shewanella)占13%,芽孢杆菌属(Bacillus)占11%,弧菌属(Vibrio)占4%,嗜冷杆菌属(Psychrobacter)占1%。不同站位TCBS菌群的组成及各菌群的相对差异明显,其中上游区域以非嗜盐或耐盐细菌为主,下游区域以嗜盐细菌和耐盐细菌为主,具有典型的河口细菌分布特征。盐度对各TCBS菌群的分布具有重要的影响。弧菌在整个河口区所占的比例不大(6%-19%)且集中在下游区域。【结论】九龙江口存在大量的条件致病菌,其中以气单胞菌属为代表的耐盐菌,对厦门海域存在陆源性污染的风险;绝大多数弧菌属于海洋土著细菌,正常情况下(非流行性弧菌病期间)非来源于九龙江冲淡水的直接污染。[英文文摘][Objective] To investigate potential pathogens in waters of Xiamen from Jiulong River,and to provide useful information for the prevention and control of potential pathogen infections. [Methods] All samples were spread on Thiosulfate Citrate Bile Salts Sucrose ( TCBS) agar plates,and then incubated at 26 ± 1℃ for 24 ± 2 h.In total 158 TCBS strains were isolated from TCBS agar plates and pure-cultivated on 2216E agar plates All strains were identified using the 16S rRNA gene- Restriction fragment length polymorphism(RFLP),16S rRNA sequence analysis,GenBank database Basic Local Alignment Search Tool ( BLAST) and phylogenetic analysis.[Results] The results show that 158 TCBS strains from the sediments of Jiulong River estuary were classfied as 7 genus,which were Pseudomonas ( 28% ) ,Aeromonas (24% ),Pseudoalteromonas (19% ) ，Shewanella ( 13% ) ，Bacillus (11% ) ，Vibrio ( 4% ) and Psychrobacter (1% ) .The composition and distribution of TCBS bacteria groups varied with stations. Non-halophilic or haloduric bacteria groups were dominant in the upper area of Jiulong River estuary，and halophilic and haloduric bacteria were dominant in the lower area,which characterized a typical estuary feature.The salinity played a key role in the distribution of TCBS groups.Vibrios did not constitute a significant proportion (6% － 19% ) of the total TCBS strains at different stations,and most of the them distributed at the lower region.[Conclusion] There were a lot of potential pathogens in Jiulong River estuary.Aeromonas,a typical genus of halotolerant bacteria,was the potentially terrigenous bacteria contamination to the waters of Xiamen.Most Vibrio specieses were marine aborigines,which was not directly contaminated from the runoff of Jiulong River.国家自然科学基金(41006117);国家海洋公益性行业专项经费项目(200805064,201205013