The influence of upwelling and water mass on the ecological group distribution of zooplankton in Zhejiang coastal waters

依据2010年4月、7月和11月对浙江中部近海上升流海域进行的海洋调查资料,运用定量、定性方法,探讨了上升流对该海域浮游动物生态类群分布的影响。结果表明:3个季节共鉴定浮游动物64种,桡足类占主要优势,包括5个生态类群,分别是暖温带近海种、暖温带外海种、亚热带近海种、亚热带外海种和热带大洋种。在种类数组成上,春季以暖温带近海种为主,夏季则是亚热带近海种和亚热带外海种居多,秋季也是亚热带种居多,其中夏季暖温带种种类数要高于春季和秋季,这一现象与同时期东黄海沿海有所不同,主要是由于上升流将一些在海洋底部度夏的暖温种带至海洋表面造成的。此外,3个季节生态类群都是以近海种为主,表明沿岸流是影响这一海域的最主要的水团。在丰度组成上,4月暖温带近海种占总丰度的98.79%,7月暖温带近海种也是组成丰度的重要部分,10月则是亚热带近海种丰度最高。丰度组成所反映的规律与种类数组成规律一致。上升流的存在导致夏季近海暖温带种大量出现,是影响这一海域浮游动物生态类群组成的重要因素;受长江径流和椒江径流的影响,近海种成为主要生态类群,是这一海域浮游动物的一个重要的生态特征。The Zhejiang region experiences coastal upwelling characterized by low temperatures and high salinities throughout the year but particularly during the summer months.The upwelling in this area brings nutrients from the bottom to the surface,providing nutrients for large numbers of breeding plankton.The large quantities of zooplankton are a major food source for many fish and consequently the offshore waters of Zhejiang are productive fishing grounds.Another effect of the upwelling is that temperate warm water species that normally occur in deeper waters and offshore species are taken along with the current resulting in shifts in local zooplankton species composition and changes to the ecological structure and function of this group.This research was based on data from three oceanographic surveys in the offshore waters of the Yushan fishery during April,July and November of 2010.Using quantitative and qualitative methods,the primary aim of this paper was to examine the influence of upwelling on the ecological distribution of zooplankton in this area.A total of 64 species mainly dominated by copepods were identified which were classified into five major ecological groups including coastal temperate warm water species,offshore temperate warm water species,coastal subtropical water species,offshore subtropical water species and oceanic tropical water species.Coastal temperate warm water species were prevalent in spring,coastal subtropical water species and offshore subtropical water species in summer and subtropical species in autumn.The higher abundance of temperate warm water species such as Calanus sinicus,Paracalanus parvus and Sagitta nagae in summer relative to spring and autumn was primarily caused by the upwelling that transported these species from the bottom to the surface.This phenomenon is very different from the hydrodynamic processes that occur in the East and Yellow China Seas.Additionally,coastal species were the dominant zooplankton group,which means that coastal currents have the greatest influence on species composition in these waters.According to the research of Hu Dun-Xin,the main power of the Zhejiang coastal upwelling comes from the upward movement of subsurface water from the Kuroshio current as it follows the continental shelf.The subsurface water of the Kuroshio current runs southeast to northwest and shifts to the east forming an upwelling when it is blocked by the Chen Island Archipelago.With low temperatures and high salinities,the subsurface water of the Kuroshio current carries temperate warm water species and offshore species.As a result,during periods of coastal upwelling temperate warm water species are carried to the surface from the bottom,producing the unusual phenomenon were the number of temperate warm water species in summer is higher than in spring and autumn,contrary to the situation in other areas that have similar climatic conditions.In terms of abundance,coastal temperate warm water species accounted for 98.79% of the zooplankton in April and also a large proportion in July,whereas coastal subtropical water species were the dominant zooplankton group in October.Upwelling leading to the appearance of large numbers of temperate warm water species is a key factor affecting the ecological composition of zooplankton in these waters.The dominance of coastal species that are influenced by Jiao-Jiang runoff was a prime ecological characteristic of zooplankton in this region.国家自然科学基金项目(40776077); 国家重点基础研究发展计划(973计划)项目(2010CB428705

柯萨奇病毒A组6型抗体中和试验Nt-ELISPOT方法的建立

目的建立快速、高效的柯萨奇病毒A组6型(CVA6)抗体中和试验Nt-ELISPOT检测法。方法取CVA6单克隆抗体(1D5)用HRP标记,以此为检测抗体,基于酶联免疫斑点检测技术(ELISPOT)建立CVA6抗体中和试验Nt-ELISPOT,通过对不同效价血清以及手足口病疑似患者血清标本的检测,比较该方法与传统抗体中和试验(NtCPE)的一致性。结果以CVA6单克隆抗体1D5为检测抗体,建立了快速检测针对CVA6中和抗体的Nt-ELISPOT检测法;该方法与Nt-CPE法相比,其检测时间显著缩短;运用这两种方法分别对4份不同效价血清样品及70份手足口病疑似患者血清样品进行CVA6抗体检测,其中和试验结果的一致性为100%。结论建立的Nt-ELISPOT方法快速、高效,可用于手足口病CVA6感染检测,为CVA6的疫苗免疫原性评价、血清流行病学调查等提供了技术支持。国家自然科学基金项目(No.31670933