Studies on the separation of a polysaccharide bioflocculant and its application in wastewater treatment

多糖生物絮凝剂是从微生物发酵液中提取、分离纯化而获得的一种胞外聚合物。由于其可生物降解、无毒、无害、降解中间体不会引起二次污染等优点而有广泛的应用。本研究以地衣芽孢杆菌CGMCC2876为出发菌株，将其胞外聚合物---多糖生物絮凝剂提取、分离纯化，最后将其应用于刚果红废水和含Pb2+废水的处理。主要研究内容及结果如下： （1）使用3倍体积无水乙醇沉淀发酵上清液，在pH=9条件下静置4h，再经过DEAESepharoseFastFlow离子交换层析和SephacrylS-400HighResolution凝胶层析后，得到一种多糖，分子量为4.47×105Da，纯度为96.4%，多糖的最终得率为...Polysaccharide bioflocculant is an extracellular polymer isolated from microbial fermentation broth. Due to its biodegradability, non-toxicity, harmlessness and no secondary pollution, polysaccharide bioflocculant has a wide range of applications. In this study, Bacillus licheniformis CGMCC 2876 was used as the starting strain, and the extracellular polymer---polysaccharide bioflocculant was isola...学位：工学硕士院系专业：化学化工学院_化学工程学号：2062014115144