Three-Dimensional Fluorescence Technique Coupled with Chemometric Second-Order Calibration Method for Simultaneous Detection of Thiabendazole and Fuberidazole in Red Wine

噻菌灵和麦穗宁是被广泛应用于预防治疗蔬菜水果病害的苯并咪唑类杀菌剂,二者的荧光光谱重叠严重,常规的荧光方法难以实现同时直接定量分析。本文将激发发射三维荧光技术分别与平行因子分析(PArAfAC)算法和交替三线性分解(ATld)算法相结合直接用于定量分析红葡萄酒中噻菌灵和麦穗宁的残留含量。当体系主成分数为3时,ATld和PArAfAC获得预测样品中噻菌灵和麦穗宁的平均回收率分别为(105.8±2.7)%、(102.1±10.1)%和(102.4±7.6)%、(103.1±9.5)%。本方法可简单、快速、经济、同时地分析红葡萄酒中噻菌灵和麦穗宁含量。Thiabendazole(TBZ) and fuberidazole(FBZ) are a class of benzimidazole fungicides and have been widely applied to protect vegetables and fruits.Conventional fl uorescence spectrum analysis method can hardly simultaneously detect TBZ and FBZ due to their serious spectral overlap.The simultaneous determination of TBZ and FBZ in red wine by excitation-emission fluorescence matrices(EEMs) combined with parallel factor analysis(PARAFAC) and alternating tri-linear decomposition(ATLD) was developed in this study.The prediction recoveries of TBZ and FBZ in the prediction set by PARAFAC and ATLD were(105.8±2.7)%,(102.1±10.1)% and(102.4±7.6)%,(103.1±9.5)% respectively,when the principal component number was 3.The method is simple,fast,low-cost,and suitable for the simultaneous determination of TBZ and FBZ in red wine.国家自然科学基金项目(21127005;21375111); 福建省科技重点项目(2009Y0046