Study of the expression of alkaline phosphatase in Amphidinium carterae Hulbert and Cofactor requirement

磷(P)是浮游植物生长发育必须的营养元素之一，当环境中的无机磷浓度不足时(磷限制)，浮游植物就会通过表达碱性磷酸酶(AP)，以分解环境中的有机磷来维持自己的生长所需。甲藻是海洋生态系统中重要的浮游植物类群，同时也是形成有害藻藻华的主要类群，开展对AP的研究可以帮助我们更清楚地了解在自然海区和形成水华过程中，甲藻利用磷的分子机制及对其自身生长的影响。碱性磷酸酶基因表达的分子机制研究在甲藻中的研究目前刚刚起步，目前该基因的报道仅限于甲藻中个别代表种，如强壮前沟藻(AmphidiniumcarteraeHulbert)，已知该基因的表达水平受环境中无机磷浓度的调控。在海洋浮游植物中存在多种不同类型的...Phosphorus (P) is one of the major nutrients required for the growth of phytoplankton, while dissolved inorganic phosphorus (DIP) is the preferred form of P, phytoplankton can utilize dissolved organic phosphorus (DOP) when DIP is limited. Under phosphate-deficient conditions, alkaline phosphatase (PtAPase) is expressed and hydrolyzes DOP to release phosphate in order to maintain cell growth and m...学位：理学硕士院系专业：海洋与地球学院_海洋生物学学号：2232012115131

Temporal and spatial variation of macrobenthic communities in the intertidal zone of Xunpu,Quanzhou Bay

为了比较泉州湾蟳埔潮间带沙滩、互花米草滩和牡蛎石泥滩3种生境(3个潮层)的大型底栖动物群落,2011年4月至2012年1月对3种生境的大型底栖动物进行了季度定量取样。在3种生境共获得85种大型底栖动物,其中环节动物39种,软体动物20种,节肢动物21种,刺胞动物、扁形动物、纽虫动物、星虫动物和脊索动物各1种。多维标度排序(MdS)分析表明,春季和冬季泉州湾蟳埔潮间带3种生境的大型底栖动物群落相似性较低;夏季和秋季互花米草滩与牡蛎石泥滩的大型底栖动物群落相似性较高,而与沙滩的大型底栖动物群落相似性较低。沙滩大型底栖动物群落的季节变化较明显,其次是牡蛎石泥滩,而互花米草滩大型底栖动物群落的季节变化较不明显。大型底栖动物栖息密度和生物量随着潮层降低而增加。单变量双因素方差分析(TWO-WAy AnOVA)表明,不同生境之间的大型底栖动物物种数、栖息密度、多样性指数、均匀度指数和丰度指数有显著差异,但生物量无显著差异,这是因为沙滩的物种数较少,栖息密度较低,但优势种弧边招潮蟹(uCA ArCuATA)个体较大,互花米草滩和牡蛎石泥滩的优势种为加州中蚓虫(MEdIOMASTuS CAlIfOrnIEnSIS),个体相对弧边招潮蟹小。不同季节之间大型底栖动物物种数、栖息密度、生物量和丰度指数有显著差异,但多样性指数和均匀度指数元显著差异,这是因为沙滩物种数少,但个体分布比较均匀,而互花米草滩和牡蛎石泥滩物种数较多,个体分布较不均匀。以上结果表明,潮汐、沉积物粒径和生境是影响潮间带大型底栖动物群落的主要因素。潮汐导致潮间带的空间异质性,空间异质性导致大型底栖动物群落的差异。This study was conducted in three different tidal levels within the intertidal zone of Xunpu,Quanzhou Bay,namely a sandy flat(on high-tide level),a Spartina alterniflora-dominated flat(on middle-tide flat) and an oysterdominated flat(on middle-tide flat).Investigations were repeated four times between 2011 and 2012.We aimed to understand the spatial and temporal variation of macrobenthic community structure and to evaluate differences among three habitats in the study area.A total of 85 macrobenthic species were recorded,including 39 annelida(38 polychaeta and 1oligochaeta),21 Crustacea,20 mollusca(6 gastropoda and 14 bivalvia),1 cnidaria,platyhelminthes,nemertea,sipuncula and chordata.The results of MDS analysis showed different macrobenthic communities among the three sites in spring and winter,while communities at the S.alterniflora-dormnated and oyster-dominated flats were similar in summer and autumn.Within each season,the species number in the S.alterniflora-dominaied flat was higher than that at the other two sites,probably due to a mixed sediment type and the shadowing of S.alterniflora acting as a shelter to animals.Instead,density and biomass of macrobenthos were higher at the two middle-tide flats than at the high-tidal flat.The temporal variation of macrobenthos was largest in the high-tidal flat and least in the S.alterniflora-dommated flat.With reduced tidal layer,density and biomass increased.Analysis of two-way ANOVA showed that in the three different habitats the species number,density,biodiversity,evenness and richness of macrobenthos were significantly different,while biomass was not.This because the species number and density were lower at the high-tide flat,dominated by the crab Uca arcuata,than at the other two sites,dominated by the polychaete Mediomastus californiensis.A comparison between different seasons showed that there were significant differences in species number,density,biomass and richness index,while diversity index and evenness index were not.This because the species number on the high-tide sand/lat was low,but distribution was more even.The results indicate that larger tidal ranges and longer submerged time in summer and autumn cause more similarity of macrobenthic communities in the S.alterniflora-dommated and oyster-dominated flats.Overall,tidal level,sediment particle size and habitat type were the main factors affecting the temporal and spatial distribution of macrobenthos in Xunpu intertidal zone,Quanzhou Bay.国家自然科学基金(41176089

用于空间科学实验柜的动力吸振器阵列

本实用新型属于减振控制技术领域，特别涉及一种用于空间科学实验柜的动力吸振器阵列。动力吸振器阵列包括空间科学实验柜及设置于空间科学实验柜上的多个电子学、热控抽屉，空间科学实验柜的后侧设有与各电子学、热控抽屉相对应的抽屉安装板，抽屉安装板上间隔设有多个动力吸振器结构，动力吸振器结构包括由内至外依次设置的阻尼层、约束层及硬涂层。本实用新型对空间科学实验柜整体结构无改变，结构形式合理，吸振效果明显，大大减小空间实验柜的振动响应

Secondary Production of Macrofauna in Mangrove and Salt Marsh of Zhangjiang River Estuary,Fujian

为了比较福建漳江口红树林和盐沼湿地不同植物生境的大型底栖动物次级生产力,依据《海洋监测规范》(gb17378—2007)的定量框采样法,采用孔径为0.5MM的网筛,于2010年1,4,7和10月在漳江口红树林和盐沼湿地4种植物生境定量采集,获得大型底栖动物物种数、栖息密度和生物量数据,运用brEy的经验公式计算不同植物生境的大型底栖动物次级生产力.结果表明:4种植物生境的大型底栖动物年平均次级生产力为4.77g/(M2.A),其中白骨壤(AVI-CEnnIA MArInA)生境大型底栖动物的年平均次级生产力最高,为7.44g/(M2.A),其次是互花米草(SPArTInA AlTErnIflO-rA)、秋茄(kAndElIA CAndEl)和桐花树(AEgICErAS COrnICulATuM)生境,分别为4.30,3.94和3.40g/(M2.A).互花米草生境大型底栖动物的P/b值,是4种生境中最高的,为1.84,其次是桐花树、秋茄和白骨壤生境,分别为1.14,1.00和0.89.互花米草生境大型底栖动物的P/b值高是因为其栖息密度以个体较小的寡毛类占优势,占大型底栖动物栖息密度的85.30%,且小个体的多毛类,如小头虫(CAPITEllA CAPITATA)和刚鳃虫(CHAETOzOnE SETOSA)比例高,分别占多毛类栖息密度的52.7%和24.9%.个体较小、生命史较短的种类P/b值较高.In order to compare the different botanic biotopes in mangrove and salt marsh of Zhangjiang River estuary,Fujian,Brey′s empirical formula was used to calculate the secondary production of macrofauna based on the macrofaunal data,including species number,density and biomass,which were sampled in four seasons in 2010 following the standards of the Specification for Marine Monitoring(GB 17378—2007).The result showed that the mean annual secondary production of macrofauna in 4 biotopes was 4.77 g/(m2·a).The secondary production of macrofauna was highest at Avicennia marina biotope(7.44 g/(m2·a)),second highest at Spartina alterniflora biotope(4.30 g/(m2·a)),third highest at Kandelia candel biotope(3.94 g/(m2·a)),and lowest at Aegiceras corniculatum biotope(3.40 g/(m2·a)).The P/B ratio of macrofauna community was highest at Spartina alterniflora biotope(1.84),second highest at Aegiceras corniculatum biotope(1.14),third highest at Kandelia candel biotope(1.00),and lowest at Avicennia marina biotope(0.89).Spartina alterniflora biotope had the highest P/B ratio because of the high percentage of oligochaeta(85.30%),which was small in individual size.In addition,the percentages of small-sized polychaetes such as Capitella capitata and Chaetozone setosa were high as well.Those species which are small in individual size and short in live history have higher P/B ratios.国家自然科学基金项目(41176089);国家自然科学基金重点项目(30930017

液相色谱串联质谱法测定百日咳和百白破疫苗中百日咳杆菌气管细胞毒素

百日咳杆菌气管细胞毒素(TCT)是一种引起百日咳及百日咳相关疫苗不良反应的毒性糖肽。尽管各国药典均规定了百日咳疫苗产品中TCT的含量限度,但均没有推荐TCT的含量测定方法。该研究发展了一种液相色谱-串联质谱法用于TCT的含量测定。实验优化了包括色谱柱类型和流动相组成在内的TCT色谱条件。虽然TCT在反相色谱模式和亲水作用色谱(HILIC)模式下均具有较好的保留,但HILIC模式与蛋白质沉淀的前处理方法兼容性更好,因此采用HILIC模式分离TCT。优化所得的方法具有较宽的线性范围(5.76~369 ng/L ),良好的重复性(峰面积的相对标准偏差不大于3.9%),各种基质中均有较好的回收率(96.4%~102.5%),且定量限是药典要求的TCT最高限量的1/1279 。将本方法用于共纯化百日咳疫苗、组分百日咳疫苗、共纯化无细胞百白破疫苗和组分无细胞百白破疫苗中TCT的检测,所有产品均未检出TCT,表明被检样品具有较好的工艺条件可避免TCT在产品中的残留

液相色谱串联质谱法测定百日咳和百白破疫苗中百日咳杆菌气管细胞毒素

百日咳杆菌气管细胞毒素(TCT)是一种引起百日咳及百日咳相关疫苗不良反应的毒性糖肽。尽管各国药典均规定了百日咳疫苗产品中TCT的含量限度,但均没有推荐TCT的含量测定方法。该研究发展了一种液相色谱-串联质谱法用于TCT的含量测定。实验优化了包括色谱柱类型和流动相组成在内的TCT色谱条件。虽然TCT在反相色谱模式和亲水作用色谱(HILIC)模式下均具有较好的保留,但HILIC模式与蛋白质沉淀的前处理方法兼容性更好,因此采用HILIC模式分离TCT。优化所得的方法具有较宽的线性范围(5.76~369 ng/L ),良好的重复性(峰面积的相对标准偏差不大于3.9%),各种基质中均有较好的回收率(96.4%~102.5%),且定量限是药典要求的TCT最高限量的1/1279 。将本方法用于共纯化百日咳疫苗、组分百日咳疫苗、共纯化无细胞百白破疫苗和组分无细胞百白破疫苗中TCT的检测,所有产品均未检出TCT,表明被检样品具有较好的工艺条件可避免TCT在产品中的残留