An Investment Value Analysis of KLM GROUP CO., LTD.

金龙汽车集团经过20年的发展，已经成为了中国客车行业的龙头企业，具有良好的业绩，然而在当前宏观经济不景气的情况下，未来能否继续保持以往的稳步增长，是投资者关注的焦点。本文选取金龙汽车作为分析对象，采用自上而下法，分别从宏观经济、行业、公司三方面展开分析，最后对金龙汽车做出合理估值，为中小投资者的投资决策提供参考。本文得出了以下几个结论：1、中国2009-2011年经济将处于复苏阶段，GDP将保持8.5%左右的年增长，2012年后慢慢步入上行区间，可能保持较高速度的增长。2、中国客车行业因自身周期性特点，在未来的2-3年内将受到宏观经济的消极影响而保持低速增长，而待到宏观经济复苏后,因处于成熟发...After about two decades of development, King Long Motor Group has become the leading enterprise of the Passenger Cars Industry of China, which has done a good performance. However, under the current circumstances of the recession of macroeconomic, it is focused more on whether it could still keep the former steady growth in the future. KING LONG MOTOR GROUP CO.,LTD. was chosen as our object to be ...学位：工商管理硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：1792007115065

有机-无机杂化多孔整体材料的“一锅法”制备

本发明涉及一种有机-无机杂化多孔整体材料的“一锅法”制备。烯基硅烷偶联剂和烷氧基硅烷偶联剂混合并预水解后，加入含烯基的有机聚合反应功能单体和有机聚合反应引发剂，使硅烷偶联剂间的缩聚反应和烯基有机功能单体与烯基硅烷偶联剂间的自由基聚合反应原位进行，制备出具特定有机官能团的、多孔的有机-无机杂化整体材料。该有机-无机杂化多孔整体材料的制备过程简单、反应温和，可根据不用的应用要求选用不同的烯基有机功能单体，制备出相应有机-无机杂化整体材料。带填

整体柱制备技术的新进展及其在蛋白质组学中的应用

整体柱是通过在柱管内原位聚合或固化的方法制备得到的具有多孔结构的整体棒状固定相，与传统的填充柱相比，具有通透性好、传质速率快、容易制备等优点，因此在分离分析领域特别是生物分离分析中发挥的作用日益增大。整体柱的制备及应用近年来也得到了快速发展，层出不穷的新型整体柱已被广泛用于色谱高效分离分析、固相萃取及酶反应器等方面，大大推动了分离分析科学的发展。本文主要总结了近五年来整体柱的制备技术及其在蛋白质组学应用中的一些最新研究进展

整体柱制备技术的新进展及其在蛋白质组学中的应用

整体柱是通过在柱管内原位聚合或固化的方法制备得到的具有多孔结构的整体棒状固定相，与传统的填充柱相比，具有通透性好、传质速率快、容易制备等优点，因此在分离分析领域特别是生物分离分析中发挥的作用日益增大。整体柱的制备及应用近年来也得到了快速发展，层出不穷的新型整体柱已被广泛用于色谱高效分离分析、固相萃取及酶反应器等方面，大大推动了分离分析科学的发展。本文主要总结了近五年来整体柱的制备技术及其在蛋白质组学应用中的一些最新研究进展

用于蛋白质分析的毛细管整体住的制备

Monolithic columns have been widely applied in capillary liquid chromatography (LC) and capillary electrochromatography (CEC) due to their easy preparations, low back pressure, convective mass transfer process and high separation performances comparing to the particle packed columns or the open tubular columns. With dependence on the nature of the precursors used, the monolithic columns can be categorized into two major types: the organic polymer-based and the inorganic silica-based monolithic columns. By taking advantages of polymeric monoliths, we have prepared a SCX monolithic column based on the functional monomer ethylene glycol methacrylate phosphate (EGMP) to trap and fractionate peptides in shotgun proteome analysis. A strong anion-exchange (SAX) capillary monolith was prepared to enrich phosphopeptides from protein digest for MALDI-TOF MS analysis. It was found that phosphopeptides could be specifically retained on the SAX column at high pH around 8 and could be eluted by 5% formic acid. Thus, the protein digests without any pretreatment could be loaded onto the SAX column under basic pH condition; after removing nonphosphopeptides by washing, the bound phosphopeptides could be eluted directly onto a MALDI target and analyzed by MALDI-TOF MS. This approach significantly simplified the analytical procedures and reduced the sample loss, a detection limit as low as 50 amol for the analysis of phosphopeptides from α-casein digest was achieved. A biphasic monolithic column was synthesized with 10 cm segment of SCX monolithic and 65 cm segment of RP monolith in a single 100 μm i.d. capillary in this study. This biphasic monolithic column was applied in online multidimensional separation for shotgun proteome analysis under relatively low operating pressure of 900 psi, and good separation performance of this biphasic column was demonstrated. A fully automated system with integration of a reversed phase-strong cation exchange (RP-SCX) biphasic trap column into vented sample injection system was developed to realize online sample loading, isotope dimethyl labeling and online multidimensional separation of the proteome samples. However, both the polymeric and silica monoliths have their own disadvantages. Recently, the organic-inorganic hybrid monolithic columns have been attracting great attention since the organic functional moieties can be incorporated into the inorganic silica monolithic matrices via the sol-gel process to give various properties, such as good mechanical stability and solvent resistance. A “one-pot” process for the preparation of organic-silica hybrid capillary monolithic columns by concurrently using organic monomers and alkoxysilanes was developed