Optimazation Dosage Study of the Combination of Chlorogenic and Geniposide on Non-alcoholic Fatty Liver Disease

目的：本研究通过运用数学模型均匀设计法，观察中药有效成分绿原酸、栀子苷配伍使用对高脂饮食诱导非酒精性脂肪性肝病（NAFLD）大鼠模型所产生的药理效应。并从多角度指标分析绿原酸、栀子苷对NAFLD脂质代谢紊乱、肝脏炎症损伤病理状态的影响，筛选出绿原酸-栀子苷组合抗NAFLD的最优剂量配比，探索中药复方治疗NAFLD的药效基础。 方法：1、均匀设计筛选实验：55只雄性Wistar大鼠，随机分为正常组、造模组。正常组普通饲料喂养，造模组采用改良高脂饲料饮食8周诱导NAFLD大鼠模型。造模组自第5周开始随机分为模型组和6组药物组。药物组以绿原酸、栀子苷作为考察因子，选用U6（64）均匀表进行组方设计...Abstract Objective: To observe the effects of the combination of chlorogenic acid and geniposide, which are effective components of Chinese medicine, on experimental rat model of Non-alcoholic fatty liver disease （NAFLD）induced by high fat diet, we applied uniform design method to establish the the mathematical model. What's more, we chose muti-angle indexs to analyse the effects of chlorogenic a...学位：医学硕士院系专业：医学院_中医内科学学号：2452012115323

名老中医周家芳治疗消化系统恶性肿瘤临证经验

遵义市名老中医周家芳行医五十余年,临证经验极为丰富。本文对周家芳老中医从燮理中焦、疏肝健脾、攻补皆施、适当运用抗癌药等方面治疗消化系统恶性肿瘤的临证经验予以介绍

Mechanism of geniposide in improving free fatty acid metabolism in rats with non-alcoholic fatty liver disease

观察栀子苷改善非酒精性脂肪性肝病的效果,从游离脂肪酸探讨栀子苷调节非酒精性脂肪性肝病的作用机制。健康雄性Wistar大鼠40只,随机分为4组:正常组、模型组、栀子苷组和血脂康组,每组大鼠10只,正常组大鼠给予正常大鼠饲料喂养,其余3组大鼠均采用高脂饲料喂养以诱导非酒精性脂肪性肝病,造模时间为8周,从第5周起至第8周末,栀子苷组和血脂康组分别灌服相应的药物。记录大鼠体重、肝湿重、脂肪质量;用相应方法检测肝组织TG,FFA,FAS,AMPK,ACCase及Malonyl-Co A含量,血清CHO,LDL-C的含量,血清AST,ALT的活性;观察肝脏组织肉眼及病理变化(HE染色法)。结果显示,与正常组相比,模型组大鼠的体重;肝湿重;脂肪重;血清CHO,LDL-C,ALT,AST;肝组织TG,FFA,FAS,ACCase及Malonyl-Co A含量皆显著升高(P<0.01);肝组织AMPK活性显著降低(P<0.01),肝组织肉眼外观及病理切片脂肪变性明显,并出现炎症损伤;与模型组相比,栀子苷组大鼠体重、脂肪质量、肝组织FFA含量、血清ALT,AST活性均显著降低(P<0.01),肝湿重,肝组织TG,FAS,ACCase及Malonyl-Co A含量明显降低(P<0.05),肝组织AMPK活性明显增多(P<0.05),肝脏肉眼外观及病理学表现均有所改善;与模型组相比,血脂康组大鼠的肝湿重、脂肪质量、肝组织TG,FFA和血清LDL-C水平明显降低(P<0.05);与血脂康组相比,栀子苷组大鼠的体重、脂肪质量、肝组织FFA含量均显著降低(P<0.01),其他方面无明显差异。结果表明,栀子苷具有显著的改善高脂饮食诱导的大鼠非酒精性脂肪性肝病的药理效应;其改善大鼠非酒精性脂肪性肝病的游离脂肪酸代谢是通过调节"AMPK-ACCase-Malonyl-Co A-FFA"轴来实现的。To observe the effect of geniposide on non-alcoholic fatty liver disease( NAFLD),and discuss the mechanism of geniposide for NAFLD from the aspect of free fatty acid,forty healthy Wistar male rats were randomly divided into normal group,model group,geniposide and Xuezhikang group. The rats in normal group were fed with normal diets,and the rats in other 3 groups were given with high-fat diet for 8 weeks to induce the NAFLD models. From the week 5 to end of week 8,the rats in geniposide and Xuezhikang group were intervened with corresponding medicines. The body weight,liver wet weight,and fat weight of the rats were recorded.Visual and pathological changes in hepatic tissues were observed with HE staining. The contents of TG,FFA,FAS,AMPK,ACCase and Malonyl-Co A in hepatic tissue,contents of CHO and LDL-C in serum and activities of AST and ALT in serum were detected by using corresponding methods. The results showed that the body weight,liver wet weight,and fat weight of the rats,CHO,LDL-C,ALT and AST levels in serum,TG,FFA,FAS,ACCase and Malonyl-Co A levels in hepatic tissues of the rats in model group were significantly higher than those in normal group( P < 0. 01),while AMPK activity was significantly lower than that of the normal group( P < 0. 01),with obvious visual and pathological steatosis in hepatic tissues,and inflammatory injury occurred in model group. Compared with the model group,body weight of the rat,fat weight,levels of FFA in hepatic tissues,ALT and AST activities in serum,liver wet weight,TG,FAS,ACCase and Malonyl-Co A levels were significantly decreased in geniposide group( P < 0. 01),while the AMPK activity in hepatic tissues was significantly increased( P < 0. 05),with improvement in visual and pathological performance.Compared with the model group,liver wet weight,fat weight,TG and FFA levels in hepatic tissues,and LDL-C level in serum were significantly decreased in Xuezhikang group( P < 0. 05). Compared with Xuezhikang group,the body weight of rat,fat weight and FFA level in hepatic tissues were significantly lower in geniposide group( P < 0. 01),but with no significant difference in other aspects. These findings indicated that geniposide was highly effective in improving the pharmacological effect of NAFLD induced by highfat diet,and the mechanism was achieved through AMPK-ACCase-Malonyl-Co A-FFA axis.国家自然科学基金项目(81274155;81503529);; 福建省自然科学基金项目(2014J01374);; 福建省高等学校新世纪人才计划项目;; 厦门市科技计划项目(3502Z20134020);; 福建省中医药科研项目(WZPW201308

焊接热输入对TMCP和QT态高强钢接头冲击韧性的影响

为高强钢在低温环境下的应用提供试验数据和建议，本文重点围绕焊接热输入和接头母材的不同交货状态这两个关键影响因素，选取控轧控冷（TMCP）和调质（QT）两种交货状态的20 mm厚Q550D和Q690D高强钢，分别进行1.0 kJ·mm-1、1.5 kJ·mm-1和1.9 kJ·mm-1三种常见的气保焊焊接热输入的接头制备、韧性试验检测和对比分析，进行了不同交货状态下对接接头的母材、焊缝区和热影响区（HAZ）的夏比冲击功及其变化规律，以及韧脆转变温度的研究。结果表明：焊接热输入对接头各位置冲击韧性的影响不明显，而交货状态不同则接头呈现出不同的冲击韧性，其中QT态钢板焊接接头热影响区的冲击韧性要优于母材和焊材，而TMCP态钢板焊接接头热影响区的冲击韧性则低于母材和焊材；对控制工况下的韧脆转变温度的研究表明，本文所用的高强钢低温敏感性较好

The Flexible Grinding Technology Based on the Electric Current Control

【中文摘要】 针对厚度为0.1～3.5mm的印制电路板在研磨过程中刚度差的问题,介绍了一种基于电流控制的柔性研磨技术.在刷辊研磨过程中,根据刷辊转动电机的瞬时测量电流与预期设定电流的比较结果,控制脉冲的发送频率与数目,从而实现伺服电机以不同速度调整磨削深度,即实现恒定磨削力的研磨过程.实验结果表明,采用电流控制的柔性研磨系统可以高效而又精确地研磨印制电路板. 【英文摘要】 A flexible grinding technology based on the electric current control is presented to resolve the problem of low rigidity of PCB on grinding,the thickness of which varies from 0.1 mm to 3.5 mm.The comparative results between the real-time and setting current during grinding control the frequency and the number of servo pulse and then the servo motor adjusts the grinding depth of cut at several different speeds,in order to realize the constant grinding force in the process of grinding.The results show that th...国家科技重大专项(2010ZX04001-162

Study on the dose ratio of Chlorogenic acid-Geniposide to treat rats with experimental fatty liver based on uniform design

目的:运用均匀设计法优化绿原酸-栀子苷组合治疗实验性脂肪肝大鼠的最佳剂量配比,探讨中药有效成分配伍配比规律。方法:运用数学模型均匀设计法对绿原酸; -栀子苷组合中的2种中药有效成分进行分组设计,分为6种组合对高脂饮食诱导的实验性脂肪肝大鼠模型进行治疗,观察各组大鼠肝脏的病理变化,并以肝组织甘; 油三酯(TG)、血清低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、总胆固醇(CHO)、谷丙转氨酶(ALT)、谷草转氨酶(A; ST)测定作为筛选指标,优选二者的最优剂量配比,并根据所得重新分组验证。结果:与正常组比较,模型组脂肪肝大鼠肝组织TG、血清LDL-C、CHO含; 量明显升高(P<0.01),血清HDL-C含量明显降低(P<0.01),血清ALT、AST活性明显升高(P<0.01),肝组织存在明显脂肪变性。; 经多元逐步回归分析,绿原酸-栀子苷组合治疗脂肪肝的最佳剂量配比是绿原酸90mg+栀子苷90mg。结论:应用均匀设计与药效学分析的方法可有效优化中; 药有效成分组合的最佳剂量配比。Objective: To explore the active ingredients and dose ratio of medicine,; combined with the optimal dose ratio of Chlorogenic acid-Geniposide to; treat rats with experimental fatty liver. Methods: The active; ingredients and optimized prescription were screened out, adopting; uniform design to divide Chlorogenic acid-Geniposide into six; combinations, by using experiment on high fat diet-induced fatty liver; rat models, and the pathological results were observed. The serum LDL-C,; HDL-C, CHO, ALT, AST and liver TG were taken as the screening-indexes.; Results: Compared with the normal groups, the serum LDL-C, CHO, ALT, AST; and liver TG in model groups were significantly higher (P<0.01), the; serum HDL-C in model group was significantly decreased (P<0.01), and the; pathology of liver tissue got obvious steatosis. According to stepwise; regression analysis of uniform design, the best ratio of Chlorogenic; acid-Geniposide to anti-NAFLD were Chlorogenic acid 90mg with Geniposide; 90mg. Conclusion: Uniform design is the appropriate way to confirm the; optimized prescription for active ingredient of Chinese medicine.国家自然科学基金面上项目; 福建省自然科学基金面上项目; 福建省高等学校新世纪人才计划项