Preparation of Upconversion/Palladium Multifunctional Nanoparticles for Photodynamic/Photothermal Therapy

癌症已成为危害人类健康的主要杀手，实现癌症的有效诊断和治疗已成为当代社会迫切需要解决的重要课题之一。近年来，具有特定功能的纳米材料在癌症的成像诊断和治疗中发挥着重要的作用。本论文针对纳米材料在肿瘤的光动力学治疗和光热治疗方面的应用主要开展了两方面的研究工作:(1)以稀土上转换发光纳米颗粒作为光敏剂的载体开展近红外光诱导光动力学治疗研究；(2)设计并合成钯纳米片/光敏剂复合纳米颗粒，用于肿瘤细胞的单激光激发下的光热-光动力联合治疗。具体内容如下： 第一章：针对纳米材料在癌症治疗中的应用，简要综述了稀土上转换纳米颗粒在光动力学治疗，光热纳米材料在光热治疗及多功能纳米材料在癌症联合治疗中的应用进展...Cancer has become the main killer of human’s health. In recent years, nanoparticles with unique functions have played an important role in cancer imaging and therapeutics. Aiming at the application of nanoparticles in cancer photodynamic therpy and photothermal therpy, this thesis mainly carries out two parts of study: (1) Application of lanthanide ion doped upconversion nanoparticles as photosen...学位：理学硕士院系专业：化学化工学院_无机化学学号：2052010115156

Preparation and photodynamic therapy application of NaYF4:Yb, Tm-NaYF4:Yb, Er multifunctional upconverting nanoparticles

E-mail Addresses: [email protected]; [email protected] preparation, characterization and application of NaYF4:Yb3+, Tm3+-NaYF4:Yb3+, Er3+ core-shell upconversion nanocrystals (UCNPs) with multiple emission peaks (e.g. 539, 654 and 802 nm) have been demonstrated in this work. The monodisperse nanocrystals were prepared via a modified thermal decomposition synthesis. The resulting UCNPs were similar to 31 nm in diameter with the lanthanide ions Tm3+ and Er3+ doped in the core and the shell, respectively. Under the laser diode excitation at 980 nm, these core-shell nanocrystals give strong upconversion emissions from the visible to near-infrared (NIR) region. By coating a PEG-phospholipid (PP) layer on the surface of the nanocrystals, the as-prepared UCNPs were favorably endowed with good water solubility for the potential biological applications. Here, a photosensitizer drug of Chlorin e6 (Ce6), which has maximum absorption that overlaps with the red emission of UCNPs, was loaded on these PP-coated UCNPs (UCNP@PP) by physical adsorption. The activity of the Ce6-loaded UCNP@PP (UCNP@PP-Ce6) in photodynamic therapy of cancer cells in vitro has been fully investigated in this work. Our results indicated that these multifunctional UCNP@PP-Ce6 nanoparticles have efficient NIR-to-NIR upconversion luminescence and photodynamic therapy capabilities, which could be potentially employed as a theranostic platform for cancer treatment.National Natural Science Foundation of China 21101131 21021061 20925103 Natural Science Foundation of Fujian Province 2012J01056 Fundamental Research Funds for the Central Universities 2010121015 Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry NFFTBS J121001

Multifunctional Core-Shell Upconverting Nanoparticles for Imaging and Photodynamic Therapy of Liver Cancer Cells

通讯作者地址: Chen, XL (通讯作者),Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Xiamen Univ, Dept Elect Sci, Fujian Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China 4. Xiamen Univ, Dept Commun Engn, Fujian Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China 电子邮件地址: [email protected]; [email protected] upconversion nanoparticles (UCNPs) have attracted considerable attention for their application in biomedicine. Here, silica-coated NaGdF4:Yb,Er/NaGdF4 nanoparticles with a tetrasubstituted carboxy aluminum phthalocyanine (AlC4Pc) photosensitizer covalently incorporated inside the silica shells were prepared and applied in the photodynamic therapy (PDT) and magnetic resonance imaging (MRI) of cancer cells. These UCNP@SiO2(AlC4Pc) nanoparticles were uniform in size, stable against photosensitizer leaching, and highly efficient in photogenerating cytotoxic singlet oxygen under near-infrared (NIR) light. In vitro studies indicated that these nanoparticles could effectively kill cancer cells upon NIR irradiation. Moreover, the nanoparticles also demonstrated good MR contrast, both in aqueous solution and inside cells. This is the first time that NaGdF4:Yb,Er/NaGdF4 upconversion-nanocrystal-based multifunctional nanomaterials have been synthesized and applied in PDT. Our results show that these multifunctional nanoparticles are very promising for applications in versatile imaging diagnosis and as a therapy tool in biomedical engineering.NSFC 21101131 21021061 20925103 20871100 Fok Ying Tung Education Foundation 121011 NSF of Fujian Province 2009J06005 Fundamental Research Funds for the Central Universities 2010121015 Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry NFFTBS J103041

Synthesis of magnetic, fluorescent and mesoporous core-shell-structured nanoparticles for imaging, targeting and photodynamic therapy

通讯作者地址: Chen, XL (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R ChinaA synthetic method to prepare novel multifunctional core-shell-structured mesoporous silica nanoparticles for simultaneous magnetic resonance (MR) and fluorescence imaging, cell targeting and photosensitization treatment has been developed. Superparamagnetic magnetite nanoparticles and fluorescent dyes are co-encapsulated inside nonporous silica nanoparticles as the core to provide dual-imaging capabilities (MR and optical). The photosensitizer molecules, tetra-substituted carboxyl aluminum phthalocyanine (AlC(4)Pc), are covalently linked to the mesoporous silica shell and exhibit excellent photo-oxidation efficiency. The surface modification of the core-shell silica nanoparticles with folic acid enhances the delivery of photosensitizers to the targeting cancer cells that overexpress the folate receptor, and thereby decreases their toxicity to the surrounding normal tissues. These unique advantages make the prepared multifunctional core-shell silica nanoparticles promising for cancer diagnosis and therapy.NSFC21021061,20925103,20871100,Fok Ying Tung Education Foundation 121011 NSF of Fujian Province 2009J06005 Fundamental Research Funds for the Central Universities 2010121015 Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministr

优化科技型企业创业生态系统的对策研究

科技型企业是推动知识创新、技术创新的主力军,是科学技术转化成生产力的主体,引入生态学的要素,对构建科技型企业创业生态系统具有重要意义。基于此,本文对科技型企业创业生态系统的功能和作用机制进行分析,并分别从科技型企业创业生态系统中起核心作用的资本、人才、技术生态位等多维度分析优化发展科技型企业创业生态系统的对策建议。</jats:p