Bioinformatics analysis of QM-like(PcQM) protein from Pseudosciaena crocea

[目的]通过分析PCQM蛋白质的结构,预测其高级结构和功能,为进一步研究PCQM蛋白提供理论依据。[方法]利用blAST P和相关分析蛋白质理化性质与结构的软件,对PCQM蛋白进行结构与功能预测分析。[结果]PCQM蛋白不存在n端信号肽,存在多个酶切位点,无跨膜结构,定位在细胞质中。不存在二硫键,该蛋白含26.05%α-螺旋结构(H),20.93%β-折叠结构(E),53.02%无规则卷曲(l)。最后,通过SWISS-MOdEl程序预测了PCQM蛋白的三级结构。[结论]较全面地分析和预测了PCQM蛋白的理化性质、二级结构和三级结构等。为深入分析PCQM在大黄鱼(lArIMICHTHyS CrOCEA)体内免疫调节功能研究,提供了理论依据。[Objective]The purpose of this study was to learn more function of PcQM protein by analyzing the AA sequence and predicting the structure of it.[Methods]Some related bioinformatics analysis software or system on internet such as Blast P,Predict Protein,Swiss-Model,was used to analyze the structure and prediction the structure and function of PcQM protein.[Results]The results showed that PcQM protein had many restriction enzyme sites.There was no signal peptide on N-terminal and no transmembrane structure.It was located in the cytoplasm.There were 26.05% α-helical(H),20.93% β-pleated sheet(E) and 53.02% random coil(L) predicted by Predict Protein.The tertiary structure was predicted by Swiss-Model.[Conclusion]The study has analyzed the sequence of PcQM protein and predicted the structure of it,which would lay a foundation for further learning the relationship between structure and function of the protein.江苏省高校自然科学研究面上项目(No.12KJD240001)资助~

不同处理对藏北地区退化人工草地垂穗披碱草种群克隆表型的影响

以不同退化程度和采用不同恢复措施的垂穗披碱草人工草地为对象,通过野外调查和室内分析相结合的方法研究了各类草地垂穗披碱草克隆种群相关表型特征的差异,结果表明,随着人工草地建植年限的延长,草地退化渐趋严重,垂穗披碱草克隆种群相关表型参数显著降低。相比较而言,封育可以提高种群的有性繁殖能力,但施肥和补播措施可以显著提高垂穗披碱草种群的克隆生长,其株高、分株数以及株丛数显著增加,而有性繁殖能力相对降低。因此,针对藏北高原以垂穗披碱草为最主要建群种或单优种群的退化草地恢复,施肥和补播应为最有效措施

广州地区女性生殖道支原体感染及其药敏状况

目的:了解广州地区女性生殖道支原体感染及药敏状况及其临床意义。方法: 收集我院妇科门诊543例宫颈分 泌物标本进行支原体培养及药敏分析。结果: 本院妇科门诊生殖道支原体感染率 60. 8%; 其中,解脲脲原体感染占 68. 9%; 新 一代大环内酯类药及喹诺酮类药对解脲脲原体的敏感性较高; 支原体混合感染可使其耐药性提高 6～ 12 倍。结论: 宫颈分泌 物支原体阳性标本的药敏试验有助于本地区妇科门诊合理用药, 减少盲目性