Studies on the Optimization of the Process for 1,3-Propanediol from Raw Glycerol by Clostridium Butyricum and Electrodialysis Desalting

1，3-丙二醇（1，3-PD）是一种重要的化工原料，具有广泛的应用领域，最主要的用途是合成PTT（聚对苯二甲酸丙二醇酯）。目前，生产1，3-PD的主要方法有化学合成法和微生物发酵法。发酵法生产1，3-PD以其利用可再生资源和对环境友好等优点日益受到重视。 本论文用丁酸梭状芽孢杆菌（Clostridiumbutyricum）发酵工业粗甘油生产1，3-PD，以降低发酵法生产1，3-PD的成本。研究内容主要包括补料分批发酵过程优化、菌株代谢进化、放大实验的考察以及电渗析脱盐的实验。 一、通过控制甘油浓度（20g/L）的补料方式进行补料分批发酵，分别考察接种量、搅拌转速、初始甘油浓度以及灭菌操作对...1,3-Propanediol (1,3-PD) is an important chemical compound with wide range of applications. It is mainly used as a monomer for the synthesis of PTT. Nowadays, the main methods of producing 1,3-PD are chemical synthesis and microbial fermentation. Microbial production of 1,3-PD has attracted wide attentions as it uses renewable resources and dose not generate pollutants. In this paper, industrial...学位：工程硕士院系专业：化学化工学院_工程硕士(化学工程)学号：2062014115146

系统的呼吸道管理对降低食管癌三野术后肺部并发症的临床研究

目的探讨系统的呼吸道管理在降低食管癌三野淋巴结清扫根治术后肺部并发症的有效性。方法将389例食管癌三野淋巴结清扫根治术患者按时间分为未行系统的呼吸道管理组与系统的呼吸道管理组,前组是护士被动地督促家属及护工进行呼吸道管理,后组采取护士专人负责系统的呼吸道管理;每组按风险程度又分为高分险组与低分险组;比较两组术后肺部并发症的发生率。结果系统的呼吸道管理组中的高风险组肺部并发症发生率明显低于未行系统的呼吸道管理组(P>0.05),低分险组肺部并发症发生率两组对比无统计学意义(P>0.05)。结论系统的呼吸道管理能有效的降低食管癌三野淋巴结清扫根治术后肺部并发症的发生率

On the system of beneficiary and beneficial estate

内容摘要 保险受益人是保险合同中重要的关系人，是保险合同利益最终的享有者，保险受益人的基本权利——受益权的行使涉及到民法诸多领域的内容，在实践中具有重要的地位。然而，由于我国对保险受益人及受益权的研究还停留在初步阶段，法律规定较滞后，无法满足社会经济及保险市场的发展需要。因此，笔者试通过比较分析与实证研究相结合的方法，就保险受益人及受益权制度中的相关问题进行考察，针对我国保险受益人及受益权制度中规定的不足之处，提出完善建议，以期能对未来保险法的修改与完善有所帮助。全文共三章。 第一章保险受益人制度。本章考察了保险受益人的涵义、资格及适用问题，阐述了保险受益人指定权的归属，指定的方式及指...ABSTRACT The beneficiary, as one of the important interested parties of the insurance contract, is the ultimate recipient of the contractual intrest. The exercise of the beneficial estate which is the basic rights of the beneficiary concern contents of a good many fields of Civil law. However, due to the fact that the research on the beneficiary and beneficial estate in China is still at its e...学位：法律硕士院系专业：法学院法律系_法律硕士(JM)学号：X20040816

pJAK2 polypeptide,an antagonist of suppressors of cytokine signaling-1,can enhance the antitumor effect of dendritic cells

目的:探讨细胞因子信号转导抑制因子-1(SuPPrESSOrS Of CyTOkInE SIgnAlIng1,SOCS1)拮抗物PJAk2多肽(氨基酸序列号为1001-1013)参与树突状细胞(dEndrITIC CEllS,dCS)的体外诱导培养后对dCS抗肿瘤作用的影响。方法:采集健康人外周血,离心获得单个核细胞,用重组人粒细胞巨噬细胞集落刺激因子(rECOMbInAnT HuMAn grAnulOCyTE-MACrOPHAgE COlOny STIMulATIng fACTOr,rHgM-CSf)及重组人白细胞介素-4(rECOMbInAnT HuMAn InTErlEukIn-4,rHIl-4)诱导dCS,第5天分为4组:单纯dCS培养(对照)组、抗原负载(lySATE-dCS)组、多肽修饰(PJAk2-dCS)组和抗原与多肽共培养(lySATE+PJAk2-dCS)组,第6天各组加入肿瘤坏死因子-α(TuMOr nECrOSIS fACTOr-AlPHA,Tnf-α)促成熟。倒置显微镜下观察dCS形态;fCM法检测dCS表型;乳酸脱氢酶(lACTATE dEHydrOgEnASE,ldH)细胞毒实验检测各组细胞毒性T淋巴细胞(CyTOTOXIC T lyMPHOCyTE,CTl)对胃癌细胞bgC-823的靶向杀伤作用;ElISA法检测白细胞介素-12(InTErlEukIn-12,Il-12)和γ干扰素(InTErfErOn-γ,Ifn-γ)的水平。结果:与未加入诱导剂组相比,各组均成功诱导出成熟dCS,均高表达Cd80、Cd83、Cd86和人类白细胞dr抗原(HuMAn lEukOCyTE AnTIgEn dr,HlA-dr),但以lySATE+PJAk2-dCS组的表达水平最高。在10:1~30:1的效靶比范围内,CTl杀伤作用与效靶比呈正相关。当效靶比为30:1时,对照组的CTl杀伤率达(19.77±2.34)%,低于其他3组(P<0.01),而lySATE+PJAk2-dCS组较lySATE-dCS组及PJAk2-dCS组都高(P<0.05)。lySATE+PJAk2-dCS组培养上清液中Il-12及Ifn-γ的分泌水平明显高于对照组(P<0.01)。结论:SOCS1拮抗物PJAk2多肽(1001-1013)可增强dCS对胃癌细胞的抗原递呈及特异性抗肿瘤作用。Objective:To investigate the effect of pJAK2 polypeptide,an antagonist of SOCS1(suppressors of cytokine signaling 1),on antitumor effect of in vitro cultivation-induced DCs(dendritic cells).Methods:Peripheral blood was collected from the healthy volunteers,and the PBMCs(peripheral blood mononuclear cells)were isolated.DCs were induced by rhGM-CSF(recombinant human granulocyte-macrophage colony-stimulating factor)and rhIL-4(recombinant human interleukin-4).On the fifth day,DCs were divided into four groups:control group,Lysate-DCs group,pJAK2-DCs group,and Lysate + pJAK2 DCs group.On the sixth day,TNF-α(tumor necrosis factor-alpha)was added into each group.The morphological features of DCs were observed under an inverted microscope;the phenotypes were detected by FCM(flow cytometry);the killing effect of CTLs(cytotoxic T lymphocytes)on gastric cancer BGC-823 cells was evaluated by LDH(lactate dehydrogenase)cytotoxicity test;the concentrations of IL-12(interleukin-12)and IFN-γ(interferon-γ)were detected by ELISA(enzyme-linked immuno sorbent assay).Results:Mature DCs presented typically morphological and phenotypic features;the DCs in Lysate + pJAK2-DCs group had the highest expression levels of CD80,CD83,CD86 and HLA-DR(human leukocyte antigen DR).When the ratio of effectors to target cells ranged from 10:1 to 30:1,the killing activity of CTLs had a positive correlation with the ratio.When the ratio of effectors to target cells was 30:1,the killing activity of CTLs in the control group was(19.77±2.34)%,which was lowest as compared with the other groups(P < 0.01),meanwhile the killing activity of CTLs in Lysate + pJAK2-DCs group was higher than those in Lysate-DCs and pJAK2-DCs groups(P < 0.05).The levels of IL-12 and IFN-γ secretion in Lysate + pJAK2-DCs group were apparently higher than those in the control group(P < 0.01).Conclusion:An antagonist of SOCS1,pJAK2 polypeptide,can enhance the ability of antigen presentation and specific antitumor effect of DCs on gastric cancer cells.南京军区医学科技创新课题资助项目(编号:10MA068); 福建省自然科学基金面上项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014

注射用泮托拉唑钠致白细胞、血小板减少1例

1病例资

三维荧光光谱总体积积分法

三维荧光光谱法是近20年来发展起来的荧光分析新技术.我们利用其丰富的信息量,提出的三维荧光光谱总体积积分法不仅较好地体现了三维荧光光谱选择性高的特点,而且灵敏度较常规法有较大的提高,从而进一步体现了三维荧光光谱的优越性.本文拟通过一系列荧光物质的检测,进一步探讨该方法的特点

不同环境介质中溴化乙锭的三维荧光光谱研究

溴化乙锭(Eb)是一种应用于dnA研究最常用及较理想的荧光探针.由于仅双链dnA能使Eb的荧光明显增强,Eb常用来作为双链dnA的结构探针.在一定意义上,生物大分子可以认为是一种有序化介质,在不同类型有序介质中Eb的三维荧光光谱特征将可以更好地帮助理解Eb与dnA的作用机理.因此系统研究Eb的荧光特性具有一定的意义.本文研究了不同环境介质中Eb的三维荧光光谱特性,这是文献未见报道的.实验发现Eb在阴离子胶束十二烷基硫酸钠(SdS)、非离子胶束聚氧化乙烯(9,5)对-特辛苯酚(TrITOn国家自然科学基金资助项

三维荧光光谱法研究蛋白质溶液构象

采用三维荧光光谱法(三维荧光光谱、三维荧光偏振光谱)结合内源荧光探针色氨酸对牛血清白蛋白(bSA)和鸡蛋白蛋白(EA)及其在不同条件下的构象变化进行了研究.结果表明,三维荧光光谱法是一种研究蛋白质溶液构象很有效的分析方法,该方法能够较直观地表明色氨酸残基在蛋白质分子中的微环境及其在不同条件下的构象变化,并得出了一些有价值的结果.国家自然科学基金资助项

一类新的表面活性剂——长链烷基三苯基鏻盐

含有荧光团的分子在溶液中簇集时,荧光团的局部浓度增大,互相间产生合适的几何关系,在荧光光谱上出现激基缔合物(excimer)或激基复合物(exciplex)荧光峰。这已显示出在研究溶液中分子的簇集时的重要作用。吴世康等曾报道在亲油基团中含有芳基的表面活性物质溶液在胶束形成过程中可观察到激基缔合物荧光,由此作为胶束形成的标志。最