Activities of several digestive enzymes of Babylonia areolata Link (Gastropoda:Buccinidae)during early development

将方斑东风螺Babylonia areolata(Link)的早期发育从早期面盘幼体到变态后15d的稚贝分成6个阶段,分别测定各阶段蛋白酶(P)、脂肪酶(L)、淀粉酶(A)和纤维素酶(C)活性。研究结果表明,随着幼体发育的推进,蛋白酶比活力逐渐减小,脂肪酶比活力逐渐增加,淀粉酶和纤维素酶比活力则在变态前逐渐增加,而变态后逐渐减小。比较A/P和(A+C)/(P+L)两种指标指示方斑东风螺幼体发育过程中的食性变化,提出(A+C)/(P+L)用于指示方斑东风螺食性变化更合理、准确。综合比较幼体发育各阶段4种消化酶活性,发现在面盘幼体向稚贝变态期间,幼体消化能力最弱,原因与幼体的变态过程有关。实验结果也验证了贝类具有分泌纤维素酶的能力,该酶在东风螺对纤维素分解中占主导地位。Specific activities of protease(P),lipase(L),amylase(A) and cellulase(C) of Babylonia areolata Link were measured respectively at 6 different stages from early veliger to juvenile 15 days after metamorphosis.The activity of protease was found to decrease gradually over time,while the activity of lipase was detected to increase step by step with larval development.The activities of amylase and cellulase increased slowly within 12 days after hatch,then increased rapidly at competent larva stage and decreased sharply after metamorphosis.A comparison was made between two indexes used as indicators of feeding habit: ratio of amylase activity to protease activity(A/P) and ratio of the sum of amylase and cellulase to the sum of protease and lipase((A+C)/(P+L)).It was concluded that the latter index((A+C)/(P+L)) was more suitable than the former one(A/P) when they were used as indicators of feeding habit.The digestion pattern of Babylonia areolata Link was revealed with the summation of 4 kinds of digestive enzyme activities at the same stage.The digestion of juvenile 3 days after metamorphosis was the weakest among the 6 stages after comparing the summations at different stages.Weak digestion may be one of the most important reasons that influence the survival rate during metamorphosis.The results of specific cellulase activity at the 6 stages indicated that the Gastropoda were able to secrete cellulase themselves.Endogenous cellulase played a vital role for Babylonia areolata to digest cellulose.科技部农业科技成果转化基金项目(03EFN213500133

The expression and clinical relevance of integrin αvβ6 and JunB in oral squamous cell carcinoma

目的:探讨整合素αVβ6、Junb在口腔鳞癌组织中的表达及其临床意义。方法:采用免疫组化方法检测120例口腔鳞癌及15例口腔正常黏膜组织中整合素αVβ6和Junb的表达情况,并分析两者表达与口腔鳞癌患者临床病理指标及预后的关系。结果:整合素αVβ6表达于口腔鳞癌组织的细胞质和胞膜,而Junb定位在口腔鳞癌组织和口腔正常黏膜组织的细胞核内。整合素αVβ6、Junb在口腔鳞癌组织中的表达强度显著高于口腔正常黏膜组织(P<0.01)。整合素αVβ6在口腔鳞癌组织中的表达与浸润深度(T分期)、组织分化程度、是否有淋巴结转移(n分期)密切相关(P<0.05),而Junb在口腔鳞癌组织中的表达与浸润深度、是否有淋巴结转移密切相关(P<0.05)。单因素生存分析表明,整合素αVβ6高表达患者的预后较低表达的患者差(P=0.021)。COX回归多因素分析表明是否有淋巴结转移是影响患者预后的独立危险因素(P<0.05)。结论:整合素αVβ6和Junb在口腔鳞癌组织中表达增加,并与口腔鳞癌的浸润、转移密切相关;整合素αVβ6表达可作为判断口腔鳞癌患者预后判断的指标。OBJECTIVE:This study aimed to investigate the expression of integrin αvβ6 and JunB in oral squamous cell carcinoma(OSCC) and the correlation with clinicopathologic characteristics and prognosis.METHODS:The expression of integrin αvβ6 and JunB protein was detected with immunohistochemical staining assay in 120 cases of OSCC and 15 samples of normal oral mucosa.The correlation with clinicopathologic characteristics and prognosis of the patients were evaluated.RESULTS:Integrin αvβ6 was detected in both cytoplasm and cellular membrane of OSCC,but JunB protein was localized in the nuclei of both OSCC and normal oral mucous tissues.The expressions of integrin αvβ6 and JunB protein in OSCC were significantly higher than that in normal oral mucosa(P<0.01).The expression of integrin αvβ6 was positively related to depth of invasion(T stage),tumor differentiation degree and lymph node metastasis(N stage)(P<0.05).The expression of JunB was positively related to depth of invasion and lymph node metastasis(P<0.05).Survival as a single factor analysis revealed that patients with over-expression of integrin αvβ6 had a shorter overall survival(OC)(P=0.021).Through Cox multivariate regression analysis,lymph node metastasis(N stage) was an independent risk factor that affected the prognosis.CONCLUSION:The over-expressions of integrin αvβ6 and JunB were common in OSCC and closely correlated with tumor invasion and metastasis.Integrin αvβ6 may be used as a prognostic marker for therapy.国家自然科学基金项目(30900661;81072208;81370160); 中央高校基本业务费专项资金项目(厦门大学); 福建省医学创新科研项目(2014-CXB-47); 福建省自然科学基金青年项

考虑行星架柔性时行星轮传动系统均载特性分析

介绍了SIMPACK中柔性体文件生成流程以及齿轮切片处理原理及相关用途;以某7MW风电齿轮箱为例,在SIMPACK软件中建立其一级行星轮系多体动力学模型;建模过程中将行星架考虑为柔性体,并对齿轮作切片处理,分析行星轮系统在额定输入功率、额定转速工况下行星架变形,以及由于行星架变形造成的齿轮不均载特性。研究发现,当使用精确的行星架三维模型,并考虑行星架柔性时,受齿轮啮合力变化以及齿轮变形的影响,不同行星轮销轴两端行星架变形量不同,总体上输入端扭转变形量大于输出端;并对比行星架变形和齿轮均载系数,发现行星架扭转变形主要影响齿轮齿向不均载系数,对齿间载荷分布影响很小。研究结果可为行星架结构优化设计提供依据

基于Visual Basic的无级变速器脉动发生机构的运动仿真

利用Visual Basic面向对象以及包含协助开发环境的事件驱动为机制的可视化程序设计的特点,进行无级变速器脉动发生机构的运动仿真。从运动仿真中对脉动式无级变速器的机构性能进行研究。已探讨出了一种四边形的高效率脉动发生机构

非圆齿轮三维齿面误差测量技术研究

为了提高非圆齿轮的测量精度，提出了一种非圆齿轮齿面的三维测量方法。根据非圆齿轮啮合原理，建立非圆齿轮的齿面模型，完成了非圆齿轮的三维实体建模。为了比较真实地反映齿面的几何形状信息，提出了一种利用截交线对齿面进行等弧长采样的方法，对非圆齿轮齿面进行网格化划分。以椭圆齿轮为例，通过Einscan-SP扫描仪得到非圆齿轮的三维实体模型，利用Geomagic Qualify软件进行体模型与理论模型的对齐，并对对齐的齿面进行网格化划分得到非圆齿轮齿面的三维误差值。此方法消除了二维定位误差对测量精度的影响，提高了非圆齿轮的测量精度

Analysis and evaluation of nutritional composition of Babylonia areolata Link and Babylonia formosae habei

本文对方斑东风螺和波部东风螺自身营养成分进行分析,测定其中的含肉率和腹足肌含有的基本营养成分,分析氨基酸种类及组成和脂肪酸种类及组成,对方斑东风螺和波部东风螺的营养价值进行综合评定以及对比分析.结果表明:方斑东风螺和波部东风螺含肉率分别为48.94%±2.01%和45.12%±2.29%,腹足肌中都含有高达70%左右的蛋白含量和5%左右低含量的脂肪,糖类含量接近10%,氨基酸种类齐全,必需氨基酸相对含量高,必需氨基酸指数在43以上,肉味鲜美,方斑东风螺的EPA和DHA相对含量总和为7.1%,波部东风螺为10.5%,方斑东风螺和波部东风螺的n-3系列不饱和脂肪酸相对含量分别高达6.4%和7.5%,n-6系列分别为13.4%和15.0%,属名贵滋补的海产品.The nutritional compositions of B.areolata Link and B.formosae habei Altena & Gittenberger were analyzed.The ratio of flesh of Babylonia were determined and muscle of nutritional compositions,amino acid compositions and contents,fatty acid compositions and relative contents were analyzed.The results show that B.areolata and B.formosae habei ratio of flesh are 48.94%±2.01% and 45.12%±2.29%,respectively.They contain around 70% protein and 5% fat,10% sugar and were rich in variety of amino acids and high essential amino acid relative contents.The essential amino acid indexes were over 43.The taste of flesh is delicious.The relative contents of EPA and DHA of muscle in B.areolata were 7.1%,and that for B.formosae habei were 10.5%.The relative contents of n-3 PUFA of muscle in B.areolata were 6.4%,and that of B.formosae habei were 7.5%.The relative contents of n-6 PUFA of muscle in B.areolata were 13.4%,and that of B.formosae habei were 15.0%.They are precious as nutritional seafood.科技部农业科技成果转化基金资助项目(03EFN213500133

Studies on Protein Requirement of Babylonia areolata Link

以白鱼粉和酪蛋白为蛋白源设计了8个不同蛋白质水平的饲料,在水温26.90±1.52℃,盐度27.6±0.5及遮光的条件下对初始个体体质量为2.16±0.05 g的方斑东风螺(Babylonia areolataL ink)进行为期60 d的生长实验.统计分析结果表明:饲料中蛋白含量对方斑东风螺的相对增质量率呈抛物线回归关系,在饲料蛋白含量为43.10%时,相对增质量率达到最大值(p0.05),蛋白质效率随着饲料中蛋白质含量的增加而降低,各蛋白组间的成活率没有显著差异(p>0.05).以相对增质量率为指标,采用回归分析法确定方斑东风螺饲料蛋白质适宜含量为36.47%~43.10%.A 60-day growth experiment was conducted in order to determine the protein requirement for the growth of Babylonia areolata Link with initial mass of 2.16±0.05g,at the temperature of 26.90±1.52 ℃ and the salinity of 27.6±0.5.White fish meal and casein were used as the main dietary protein source to formulate experimental diets at eight protein levels.The results showed that the relationship between the feed protein requirement and the relative mass gain of Babylonia areolata Link was parabola regression.The relative mass gain of Babylonia areolata reached its maximum when the dietary protein level was up to 43.10%.(p0.05).The protein efficiency ratio tended to decrease with the increasing dietary protein level.There was no significant difference in the survival rate.(p>0.05).Based on the analysis of regression equation and using the relative mass gain as index,we could determine that the optimal protein requirement of Babylonia areolata Link should be at the range of 36.47%~43.10%.科技部农业科技成果转化基金(03EFN213500133)资