The Experimental Study of Magnetic Resonance Immunoimaging Using Anti human Lung Cancer Monoclonal Antibodies Coated Superparamagnetic Iron Oxied Nanoparticle

目的：探讨标记抗人肺癌单抗的超顺磁性氧化铁微粒（SPIO）进行磁共振免疫显像的可能性。材料与方法：将采用fE（Ⅲ）与fE（Ⅱ）混合液在碱性条件下制备SPIO微粒；将粒径4.5nM±3.4nM的SPIO微粒标记到抗人肺癌单抗（MCAbS）上；观察静注SPIO—MCAbS定向改变载瘤裸鼠（n＝4）Mr信号强度的可能性。结果：SPIO—MCAbSMr成像能有效地降低全部瘤靶的Mr信号。结论：磁共振免疫成像是一种有发展前景的肿瘤靶向显像新途径Objective: To investigate the possibility of MR immunoimaging using monoclonal antibodies against human lung cancer (McAbs) labelled with superparamagnetic iron oxide nanoparticle (SPIO).Materials and Methods:SPIO with a mean diameter of 4.5nm±3.4nm, was synthesized by reaction of Fe(Ⅲ) and Fe(Ⅱ) with dextran under the alkaline condition.The McAbs coupled convalently to SPIO was achieved with the potassium periodate method.Then the McAbs—SPIO was injected intravenously in nude mice hosted human lung cancer (n=4).Results:The MR contrast agent of SPIO—McAbs can selectively reduce the signal intensities of the tumor targets.Conclusion: The data indicate MR immunoimaging may become a new approach For speciFic imaging of tumors.国家自然科学基

MR Immunoimaging Using Antihuman Lung Cancer Monoclonal Antibodies Labelled Gadolinium

目的：探讨gd标记抗肺癌单抚对荷瘤裸鼠的定向增强效应。材料与方法：用dTPA环二酸研与抗肺癌单抗3d3和gd3--3+分别偶联，制备出gd-dTPA-Igg：建立6只荷瘤裸鼠模型；将gd-dTPA-Igg按2Mg单抗/只的剂量从尾静脉内注入；观察平扫及注药后6小时瘤体磁共振信号。结果：gd-dTPA-Igg能选择性增加瘤灶的信号强度。结论：磁共振免疫成像为肺癌定性提供一种潜在新的方法。Purpose: To explore the Possibilitv of specific contrast enhancemen1 in nude mice hosted lung cancer using Gd labelled monocloa1 antibodies against lung adenocarcinorna.Materials and Methods: Uslng cyclic anhydride DTPA as a bifunctional chelat1ng a- gent, Gd3+ was attached to a monoclnal antihody (3D3).6 nude mlce carrying the 1ung cancer were esta[)lished.Then, Gd - DTPA - IgG was intravenously injected at a dose of 2mg IgG/mouse.MR scans wcre performed before and 6 hours after administration of Gd - DTPA - IgG.ResultS: all tumor of nodules were specifically cnhancecl with Gd - DTPA - IgG.ConcIusion: MR immunoimaging is a promise method for detecting lung cancer