Effects of iron electrovalence and species on growth and astaxanthin production of Haematococcus pluvialis

Corresponding author. [email protected] increase the cell concentration and the accumulation of astaxanthin in the cultivation of Haematococcus pluvialis, effects of different iron electrovalencies (Fe2+-EDTA and Fe3+-EDTA) and species (Fe-EDTA, Fe(OH) 3x2xand FeC6H5O7) addition on cell growth and accumulation of astaxanthin were studied. Results show that different iron electrovalencies have various effects on cell growth and astaxanthin accumulation of H. pluvialis. Compared with Fe3+-EDTA, Fe2+-EDTA stimulate more effectively the formation of astaxanthin. The maximum astaxanthin content (30.70 mg/g biomass cell) was obtained under conditions of 18 μmol/L Fe2+-EDTA, despite the lower cell density (2.3×105 cell/ml) in such condition. Fe3+-EDTA is more effective than Fe2+-EDTA in improving the cell growth. Especially, the maximal steady-state cell density, 2.9×105 cell/ml was obtained at 18 μmol/L Fe3+-EDTA addition. On the other hand, all the various species of iron (EDTA-Fe, 32x x Fe(OH) , FeC6H5O7) are capable to improve the growth of the algae and astaxanthin production. Among the three iron species, FeC6H5O7 performed the best. Under the condition of a higher concentration (36 μmol/L) of FeC6H5O7, the cell density and astaxanthin production is 2 and 7 times higher than those of iron-limited group, respectively. The present study demonstrates that the effects of the stimulation with different iron species increased in the order of FeC6H5O7,32x x Fe(OH) and EDTA-Fe.Supported by Xiamen Scientific and Technologic Projects (No.3052Z20031086); Xiamen University Alumni Association Foundation in Singapore;the First National College Students Innovative Experimental Projec

Progress in Anthropogenic Carbon Estimation,Spatial and Temporal Distribution in the Ocean

海洋是全球最大的碳汇,确定海洋中人为碳含量及其时空变化是一件具有挑战性的工作。就当前海洋人为碳含量确定方法、人为碳储量及其随时间变化进行综述总结。研究表明海洋仍然可以吸收并储藏大量的人为碳,但未来对于海洋吸收储藏人为碳的研究仍具有诸多不确定性。已有证据表明稳态海洋的概念不完全正确,需要在非稳态条件下重新定义人为碳并开展研究。同时需要积累更多的观测数据,采用包括观测和模式模拟等手段来研究不同时间尺度的人为碳的吸收和储藏过程。The ocean is the largest carbon sink in the world.It is difficult to determine the content of anthropogenic carbon( Cant) and the spatial and temporal distribution in the ocean.The methods of Cantdetermination,inventories estimation of Cant,temporal variations of Cantwere summarized in this review.The ocean can still uptake and store Cantfrom the atmosphere.However there are still many uncertainties for the oceanic Cantuptake and storage studies.The evidences show that it is not true for Cantestimations based on the steady state assumption.Thus,it is necessary to define and study the Cantbased on non-steady state.Meanwhile,it is necessary to collect more observation data and use more approaches such as observations and model simulations to study the Cantuptake and storage in different time scales.南北极环境综合考察与评估专项(编号:CHINARE2012-2015:01-04-02;01-02-01;03-04-02); 国家自然科学基金重点项目“南大洋N2O源汇格局:驱动机制及其对海洋N2O收支的影响”(编号:41230529)资

Effect on Photoluminescence Intensity of Porous Silicon Processing by a Wet Oxidized Technology

【中文文摘】在适当条件下氧化多孔硅是提高多孔硅发光强度的良好途径。首次采用含CH3CSNH2的HF酸水溶液作为氧化剂对初始多孔硅进行了湿法阳极氧化,大大改善了多孔硅的发光强度,并研究了氧化电流密度、氧化温度、氧化时间等一系列因素对氧化多孔硅光致发光强度的影响。实验发现,在电流密度1mA/cm2,氧化液温度60℃,氧化时间为10min的条件下,可以获得最强光致发光;在此最优条件下得到的氧化样品较初始样品发光增强了18倍。 【英文文摘】Oxidizing porous silicon in proper condition is a good approach to improve PS photoluminescence intensity.We for the first time using electrolyte containing CH_3CSNH_2 to oxidize initial PS,which boost up photoluminescence intensity of PS greatly.We also studied oxidizing current density,oxidizing temprature,oxidizing time etc.It is testified that the best intensity of PL can be obtained when oxidizing for 10 minutes in 60 ℃ oxidizing solution using 1 mA/cm~2 current density.In these conditions we can obtain best photolumine scence intensity which is 18 time s than that of original porous silicon

饲用水产微生态制剂的研发与应用

近20年来我国水产养殖业得到了迅猛发展,规模化、规范化、集约化水产养殖除了带来可观的经济效益外,也造成了水产养殖动物的应激加剧,机体的免疫力和抗病力下降,水产动物疾病频发,抗生素滥用等问题也伴随而生。饲用水产微生态制剂是一种绿色饲料添加剂,其因具有安全性、有效性、质量更可控性和环境友好性等特点而被当作新兴的抗生素可能替代物。本文简要概述饲用水产微生态制剂研发及其应用效果提升的技术关键,以期为进一步完善、推广饲用水产微生态制剂提供参考。厦门南方海洋研究中心海洋产业核心和关键技术攻关项目[高效饲用海洋动物源益生菌的产业化开发及应用示范.NO.17GZP007NF03

浆细胞样树突状细胞在宿主抵御隐球菌肺部感染中的作用及机制研究进展

肺隐球菌病是由隐球菌感染引起的常见真菌病,由于症状的非特异性,临床上诊断较为困难。作为条件致病性真菌感染,肺隐球菌病的结局主要与宿主免疫力有关。目前肺隐球菌病免疫学发病机制研究主要局限在T细胞和巨噬细胞。近年研究表明,作为树突状细胞亚群之一的浆细胞样树突细胞,由于其激活后可以产生大量的I型干扰素并活化相关的T细胞,所以在机体抵抗病毒和细菌免疫中发挥着重要的作用。但是浆细胞样树突细胞在真菌病,尤其是在隐球菌病的发生发展中发挥的作用尚不明确。本文将介绍肺隐球菌病的临床表现、诊治及T细胞和巨噬细胞在肺隐球菌病中的免疫机制,并通过介绍肺隐球菌病和浆细胞样树突细胞及二者之间已有报道的联系,初步阐述浆细胞样树突细胞在肺隐球菌病免疫学发病机制中的相关作用。国家自然科学基金(31770161);;上海市科学技术委员会基金(14DZ2272900);;国家卫生部基金(2018ZX10101003);;上海市科委技术标准专项(16DZ0500401)~

Nutrition Content and Indicator Value of Chlorion for Groundwater in Xiamen Island

该文以厦门岛地下水为研究对象 ,研究了地下水中营养盐含量的空间分布和变化。结果表明 2 0 0 1年DIN(无机氮总 -N)含量的变化范围是 2 0 1 2～ 136 8 1μg dm3,PO4 -P含量的变化范围是 9 2～ 1190 0 μg dm3。从 1999年至 2 0 0 0年 ,DIN含量的变化范围是 74～ 2 0 6 8μg dm3,PO4 -P含量的变化范围是 70 9～ 182 5 μg dm3。同时该文将地下水中氯离子作为海水入侵指标 ,根据氯离子背景值确定出海水入侵氯含量指标值。 【英文摘要】 The paper takes the groundwater as research object in Xiamen Island.Regional distributions of the nutrition content (NH 3-N、NO 2-N、NO 3-N and PO 4-P)in the groundwater are studied. The results showed that content of DIN for 2001 year ranges from 201.2μg/dm3 to 1368.1μg/dm3 and content of PO 4-P ranges from 9.2μg/dm3 to 1190.0μg/dm3, in Xiamen Island. From 1999 to 2000 year,in Zengcuoan village, DIN ranges from 74μg/dm3 to 2068μg/dm3 and PO 4-P ranges from 709μg/dm3 to 1825 μg/dm3.Chlorion was used as..

Characteristics and assessment of environmental quality in seawater and sediment of Yacheng Bay,Fujian

于2005年10月首次调查了福建牙城湾海域水体及沉积物的环境质量。结果表明,海水中主要超标污染物为PO4-P和d In,其含量范围分别为0.029--0.037 Mg/l和0.270--0.510 Mg/l,其它指标均符合二类海水水质标准。沉积物中各项指标基本达到《海洋沉积物质量》的一类标准,其中Hg、Pb、Cu和Cd等四种重金属的平均含量分别为0.091x10-6、39.7x10-6、29.3x10-6和0.118x10-6,有机物和硫化物的平均含量分别为0.941x10-2和207x10-6。湾水水体已达到富营养状态,且n为限制因子。此外,对沉积物中重金属及其他环境因子间的相关性进行了分析。The concentrations of the major environmental items in surface seawater and sediment are reported for the first time in Yacheng Bay in Fujian Province in Oct.2005.The major pollutants in seawater are DRP and DIN,and their concentrations are 0.029～0.037 mg/L and 0.270～0.510 mg/L,respectively.The contents of other environmental items in seawater are within the Second Class Standard of Seawater Quality.Most of the items in surface sediments are below the values of First-class Standard for Marine Sediments Quality.The average concentrations of Hg,Pb,Cu,Cd in surface sediments are 0.091×10-6、39.7×10-6、29.3×10-6 and 0.118×10-6,respectively.The organic matter and sulfide are 0.941×10-2 and 207×10-6,respectively.It is found that the quality in seawater in Yacheng Gulf was in eutrophication condition using the way of index E,while the limit factor is N.What's more,the correlations among some heavy metals and other environmental items were also analyzed.国家自然科学基金(40306012);福建省重点科技项目(2005Y021);福建省海洋与渔业局科技项目(1020-K53011;0051-K37011

Distribution pattern of PAHs in Jiulong River Estuary by both measurement and simulation

于2011年12月(冬季)在厦门九龙江河口及西港采集9个表层海水水样,采用固相萃取—气质联用方法(SPE-GC-MS)分析其中16种多环芳烃含量。研究结果表明,总溶解态态∑PAH含量为157.9~858.0 ng/L。在河口区,随着盐度升高,PAHs含量逐渐降低。基于比值法分析,表明厦门九龙江及西港海域海水中的PAHs来源存在多种途径,呈现混合来源的态势。利用LEVEL III逸度模型研究菲,芘和苯并(a)芘在各介质间的分布以及水——气界面的交换通量。模拟结果与本文实测和文献中的实测值相吻合。在16℃时,三种多环芳烃的大气沉降通量分别为17.38,7.86和8.38μg/day/m2。其中菲在大气沉降中占主导地位,约三分之二。三种多环芳烃的大气沉降通量均随温度升高而减少。当温度高于32℃时,苯并(a)芘开始从水体释放。The concentration of 16 polycyclic aromatic hydrocarbons( PAHs) of nine water samples,collected in the Jiulong River Estuary in December,2011,were analyzed using the SPE-GC-MS method. The results showed that the concentration range of total dissolved PAHs( ΣPAHs) was from 157. 9 to 858. 0 ng / L. The horizontal distribution illustrated that the concentration of ΣPAHs decreased as salinity increased. Based on ratio analysis,PAHs have complicated sources. A Level III fugacity model was developed to simulate the multimedia fate of PAHs and to study the air-water exchange fluxes of three PAHs( phenanthrene,pyrene,benzo[a]pyrene) in the Jiulong River Estuary. The calculated concentration of PAHs was similar to that measured in this study or reported in earlier literature. At 16℃,the net atmosphere deposition fluxes of these PAHs were 17. 38,7. 86 and 8. 38 μg / day / m2. Phenanthrene was predominant in the atmospheric deposition. The net atmospheric deposition fluxes of these PAHs all decreased with tem-perature. When the temperature was above 32. 0℃,benzo[a]pyrene was released from the water into the air.国家自然科学基金(40776044);; 福建省自然科学基金(2012J05078);; 福建省杰出青年科学基金(2014J06014);; 中央高校基本科研专项(20720140507

不同波段可见光对雨生红球藻生长与虾青素积累的影响

厦门市科技项目(3052Z20031086); 国家大学生创新性实验计划(2007); 福建省大学生创新性实验计划(2007); 厦门大学大学生创新性实验计划(2007); 新加坡厦门大学校友基金会项目(2007)资

Spatial distribution of per- and polyfluoroalkyl compounds in coastal waters from the East to South China Sea

The spatial distribution of per- and polyfluoroalkyl compounds (PFCs) were investigated in coastal waters collected onboard research vessel Snow Dragon from the East to South China Sea in 2010. All samples were prepared by solid-phase extraction and analyzed using high performance liquid chromatography/negative electrospray ionization-tandem mass spectrometry (HPLC/(-)ESI-MS/MS). Concentrations of 9 PFCs, including C4 and C8 (PFBS, PFOS) perfluoroalkyl sulfonate (PFSAs), C 5-C9 and C13 (PFPA, PFHxA, PFHpA, PFOA, PFNA, PFTriDA) perfluoroalkyl carboxylates (PFCAs), and N-ethyl perfluorooctane sulfonamide (EtFOSA) were quantified. The 危PFC concentrations ranged from 133 pg/L to 3320 pg/L, with PFOA (37.5-1541 pg/L), PFBS (23.0-941 pg/L) and PFHpA (0-422 pg/L) as dominant compounds. Concentrations of PFCs were greater in coastal waters along Shanghai, Ningbo, Taizhou, Xiamen and along coastal cities of the Guangdong province compared to less populated areas along the east Chinese coast. Additionally, the comparison with other seawater PFC measurements showed lower levels in this study. 漏 2011 Elsevier Ltd. All rights reserved