Effects of Diosmetin from Carbonized Cirsium japonicum on Apoptosis in Human MCF-7 Breast Cancer Cells and its Mechanisms

研究大蓟炭中香叶木素诱导人乳腺癌MCF-7细胞凋亡及其作用机制。采用硅胶和Sephadex; LH-20柱层析从大蓟炭中分离鉴定了三个黄酮类化合物,经NMR和MS鉴定他们的结构分别为香叶木素(1)、刺槐素(2)和柳川鱼黄素(3)。采用MT; S方法检测不同浓度的香叶木素对MCF-7的细胞活力的影响;流式细胞术检测不同浓度的香叶木素处理对MCF-7细胞凋亡的作用;Western; blot法检测香叶木素处理对细胞PARP、P-JNK等细胞凋亡相关蛋白表达的影响。MTS及流式细胞术结果显示香叶木素能显著抑制MCF-7的增殖并; 且诱导细胞凋亡;香叶木素可上调P-JNK促进细胞凋亡。结果表明香叶木素在体外实验能通过激活JNK细胞凋亡通路抑制MCF-7的增殖及促进细胞凋亡。To study the effects of diosmetin from carbonized Cirsium japonicum on; apoptosis in human MCF-7 breast cancer cells and its mechanisms. Three; flavonoids were isolated and purified from carbonized C. japonicum by; silica gel and Sephadex LH-20 chromatography methods. Their structures; were elucidated by NMR and MS spectroscopic data and identified as; diosmetin(1),acacetin(2) and pectolinarigenin(3). MTS assay was; performed to detect the viability of MCF-7 cells treated by different; concentrations of diosmetin. The cell apoptosis rate was further; analyzed by flow cytometry(FCM). Western blot assay was applied to; measure the apoptosis related protein expression levels of PARP,P-JNK.; Diosmetin treatment on MCF-7 cells significantly inhibited cell; proliferation and induced cell apoptosis. Diosmetin significantly; downregulated P-JNK and upregulated cleaved-PARP protein expression.; Diosmetin inhibited MCF-7 cell proliferation and induced cell apoptosis; by activation of JNK pathway in vitro.江西中医药大学校级科研项目; 国家自然科学基金; 江西省自然科学基

Vapor-liquid-equilibrium and volume expansion calculations of CO_2/acetone,CO_2/ethanol and CO_2/ethanol/water at high pressure by using t-mPR equation of state

作者简介:蒋茂星(1983－),女,助教,主要研究方向:超临界流体技术 通信联系人:李军,教授,主要研究方向:超临界流体技术,[email protected][中文文摘]采用t-mPR状态方程(EoS)对CO2/丙酮、CO2/乙醇二元体系和CO2/乙醇/水三元体系的高压汽液平衡(VLE)、液相体积膨胀率进行计算,并与文献报道的实验数据,以及采用PR EoS得到的结果进行比较。结果表明,与PR EoS相比较,t-mPR EoS可以对上述二元体系的VLE取得较好的关联效果,对三元体系取得较好的预测效果。基于VLE的计算,进一步对上述体系的液相体积膨胀率进行预测。与PREoS相比较,t-mPREoS对二元体系的预测结果的精度有所提高,对三元体系在7.8MPa左右有最大体积膨胀率,模型预测较好。[英文文摘]The vapor-liquid equilibirum (VLE) and volume expansion of the liquid phases for the CO2/acetone and CO2/ethanol binary systems and the CO2/ethanol/water ternary system have been investigated using the t-mPR equation of state (t-mPR EoS). Calcualted results have been compared with published experimental data and those obtained from the PR equation of state (PR EoS). The correlations for the published VLE data of the above binary systems and the prediction for the ternary system showed that both the t-mPR EoS and the PR EoS can provide fairly good VLE calculations, but t-mPR EoS gives slightly better results. The volume expansion prediction for the above systems and the comparison with the published data showed that the t-mPR EoS gives better results to PR-EoS for the binary systems and provides more reasonable results around 7.8 MPa (there exists a maximum volume expansion) for the ternay system.高等学校博士学科点专项科研基金资助项目(20100121110009

关于“经济发展方式转变进程中的财税政策”的探讨(笔谈)

转变经济发展方式是当前中国的热门话题,而上海在城市发展中又肩负着率先转变的重要任务,在这一意义和背景下,2010年4月24日由上海金融学院学院主办、上海金融学院公共经济管理学院承办的“《中国城市财政发展报告2009/2010:促进‘两个中心’建设的上海城市财政》首发式暨‘经济发展方式转变进程中的财税政策’论坛“在我校举行。与会专家围绕经济发展方式转变进程中的财税政策的“趋势与目标“、“机遇和挑战“、“实施方略“和“地方实践“等四个专题进行研讨,各抒己见,智慧交锋。现将其真知灼见整理摘要与读者分享,以期待更多学者共同关注当前经济发展方式转变进程中的财税政策

果树库源关系改变对源叶光合作用的影响机制研究进展

为了解果树库源关系中叶片末端产物含量的改变对光合作用的调控机制,归纳和总结了库源关系改变后,果树中叶片末端产物的积累对光合作用的几种可能的调控机制,指出了反馈抑制等假说存在的不足,并认为气孔导度为启动因子,温度为作用因子,实现对光合作用的调控是可能的途径之一,最后提出了今后的研究重点

京津冀风沙源区沙化土地治理关键技术研究与示范

京津冀风沙源区是我国北方生态屏障的重要组成部分。面向我国北方风沙区沙化土地综合治理、典型脆弱生态修复与保护等重大科技需求,京津冀风沙源区沙化土地治理关键技术研究与示范项目将综合运用长期定位观测、控制实验、多源遥感数据融合、技术示范等方法,重点研究京津冀风沙源区土地沙化形成机制与生态修复机理,研发一批沙化土地治理与产业化关键技术,并在各治理区开展试验示范,集成京津冀风沙源区沙化土地治理与产业化技术体系,构建沙化土地综合整治空间决策支持系统,为京津风沙源治理工程建设、保障京津冀地区生态安全及满足2022年北京冬奥会生态需求提供科技支撑

γ-干扰素释放试验和PPD试验在处置学校结核病暴发疫情中的应用分析

近年来,我国各地在校学生结核病的暴发疫情时有发生[1-3],给学生的身心健康造成了极大伤害。在调查处置学校结核病暴发疫情中,γ-干扰素释放试验和PPD试验作为细胞免疫学诊断结核的主要方法之一,分别通过外周血T淋巴细胞释放γ-干扰素和体内T淋巴细胞产生免疫应答来判断个体感染结核菌程度[4],为筛查发现高危密切接触者、及时控制疫情提供了科学依据。为比较γ-干扰素释放试验

Determination of Alkylphenols in Water by Solid-phase Extraction with on-Column Derivatization Coupled with Gas Chromatography-Mass Spectrometry

以烷基酚(APs)主要降解产物辛基酚(4-t-OP)、壬基酚(4-n-NP)为研究对象,建立了固相萃取(SPE)柱上衍生化、气相色谱-质谱(GC-MS)法测定水中APs的分析方法。以C18柱为固相萃取柱、N,O-(三甲基硅)三氟乙酰胺(BSTFA)为硅烷化试剂,设计五因素四水平正交实验L16(45),对衍生化影响因素、衍生化溶剂、衍生化时间以及SPE主要影响因素pH值、盐度和洗脱剂进行优化;在优化条件下,方法的回收率(高于80%)和重现性(RSD低于10%)结果令人满意,4-t-OP和4-n-NP的仪器检出限分别为3.35ng/L和6.38ng/L。采用建立的方法,回收率略高于传统的SPE萃取衍生法,具有有机溶剂用量少,方法简单快速、灵敏度高的特点,适用于河水和海水中痕量烷基酚的快速测定。An analytical methods based on solid-phase extraction(SPE)with in situ derivatization followed by gas chromatography-mass spectrometry has been developed for the determination of alkylphenols(APs)in water samples.Two important APs,4-n-nonylphenol and 4-t-octylphenol,were selected as the target compounds for method development.The SPE extraction was conducted on a C18 extraction cartridges with on column silylation by bis(trimethylsilyl)trifluoroacetamide.Operationasl parameters including silylation reagent silylation time,pH value,concentration of salts,elulent which are expected to impact on the recoveries of analytes were optimized by orthogonal experimental design.It is shown that the acetone is the best silylation reagent while DCM was efficient in eluting APs from SPE cartridges.The recovery of APs increases slightly with increasing concnetration of NaCl in the range of 0-1.5% of NaCl,beyond the concentration of 1.5%,the recovery of APs starts to decrease.But the recovery of APs reduced by the increase in pH value.The optimised method was further verified by performing spiking experiments in ultrapure water and seawater matrices,with good recovery and reproducibility for all the selected compounds.The method showed better performance than the reported liquid liquid extraction-derivatization method in terms of analytical speed,procedural simplicity and recovery.国家重点创新项目(836)(No.2003AA635180

黄土高原不同类型旱区旱作粮田深层土壤干燥化特征

随降水量趋势性减少和粮食产量不断提高,黄土高原旱作粮田深层土壤干燥化现象日益显现。在黄土高原不同类型旱区,测定了32类旱作粮田0~600 cm土层土壤湿度,分析和比较了各类粮田深层土壤贮水量、土壤湿度剖面分布和土壤干燥化强度。结果表明:①32类旱作粮田0~600 cm土层土壤湿度、土壤贮水量和土壤有效贮水量分别为13.90%、1084.4 mm和573.7 mm,其中有16类粮田发生了土壤干燥化现象,土壤水分过耗量平均值85.1 mm;②有28类旱作粮田100~400 cm土层为土壤干层,其中夏粮田土壤干层厚度大于秋粮田,最大耗水深度接近或超过600 cm;③32类旱作粮田和16类干燥化粮田土壤干燥化指数分别为110%和83%,分别属于无干燥化和轻度干燥化强度,土壤干层厚度平均值为267 cm,以半干旱偏旱区粮田土壤干燥化程度最严重