Evaluation on difference of therapeutic efficacy of Jiawei Xiaoyao Granules and Pills in treatment of emotional disorder during perimenopause based on Greene Climacteric Scale

目的:在前期加味逍遥丸治疗围绝经期情绪障碍具有比较优势的基础上,基于grEEnE量表探讨其颗粒剂和丸剂不同剂型间疗效差异。方法:经筛选后患有情绪障碍的围绝经期女性75例随机等分为A、b、C3组,分别对应采用帕罗西汀、加味逍遥丸和加味逍遥颗粒剂治疗8周。所有患者在治疗前后各进行1次grEEnE及其子因子评定,并在观察期结束时进行TESS评定。结果:grEEnE总分差及其焦虑、抑郁因子分差:3种治疗方法均能改善,且相互间无显著差异;grEEnE性因子和血管因子分差:后二组均优于A组(P<0.01);TESS评分:与A组比较,b、C组均有统计学意义(P<0.01),评分均小于A组,且后二组间无显著差异。结论:加味逍遥的2种剂型间疗效无显著差异,考虑到帕罗西汀的不良反应,治疗该病加味逍遥的2种剂型都值得推荐。Objective: To investigate the difference in therapeutic efficacy between Jiawei Xiaoyao Granules and pills based on the preliminary research of treating emotional disorder during perimenopause with Jiawei Xiaoyao Pills and Greene Climacteric Scale.Methods: 75 female patients with emotional disorder during perimenopause were slected and randomly divided into 3 groups as group A, B and C.Patients in the 3 groups were treated with paroxetine, Jiawei Xiaoyao Pills and Jiawei Xiaoyao Granules respectively for 8 weeks.Greene and Greene factors assessment were carried out in all the patients before and after treatment, and the TESS assessment was carried out at the end of the observation period.Results: The three therapies all could improve the gap in total score of Greene and its factors as anxiety and depressed, and the difference among these three groups was no significant.The factors of Greene and gap in score of vascular factors of group B and C was better than that of group A(P<0.01).The difference in TESS score between group A and group B and C was significant(P<0.01), while the difference between group B and group C was not significant.Conclusion: The difference in curative effect between the two formulations was not significant.Because of the adverse reactions of paroxetine, in the treatment of emotional disorder during perimenopause, the two formulations of Jiawei Xiaoyao Powder were worth to be recommended.国家自然科学青年基金项目(No.81302960)~

DiFFerentiation of Original Plants of Xihuangcao by Using Random AmpliFied Polymorphic DNA (RAPD) Markers

采用rAPd技术对中药溪黄草类4种原植物进行鉴定研究，结果表明rAPd技术是一种有效的中药鉴定方法，对药材的“地道性”研究有重要意义Four original plants of Xihuangcao have been diFFerentiated From each other by random ampliFied polymorphic DNA polymorphism.The results show that RAPD technique is an eFFective method For identiFication and quality study of Chinese medicinal materials.广东省自然科学基金;国家杰出青年基

Si, Ag, F离子共修饰HA纳米生物薄膜的制备与表征

采用单电流阶跃电化学沉积技术，在商业纯钛(CP-Ti)表面构建硅、银、氟离子共修饰羟基磷灰石(Si-Ag-F-HA)纳米复合薄膜。Ag+的持续释放可以提供有效的抗菌性，Si4+作为生物活性元素可以有效地抵消Ag+的潜在细胞毒性。采用电感耦合等离子体质谱法(ICP-MS)测定涂层中硅和银元素的释放规律。采用傅里叶变换红外光谱(FTIR)、扫描电子显微镜(SEM)、能量弥散X射线谱(EDS)、X-射线衍射(XRD)等技术对得到的材料进行了表征。结果表明：Si，Ag和F三种元素均匀地掺杂到了HA的晶体结构中。Si-Ag-F-HA为纳米级的针状晶体结构，薄膜整体致密且均匀。Si-Ag-F-HA纳米生物薄膜可以在一周内很好地诱导类骨磷灰石的形成，具有优异的生物活性。塔菲尔曲线测试结果证实涂层的耐SBF腐蚀性较好。ICP-MS测试结果表明Si-Ag-F-HA纳米生物薄膜可以提供持续的Si和Ag离子释放。FTIR 和ICP-MS等光谱技术为开发新型抗菌硬组织修复材料提供了高效快速的检测手段。 In this study, a novel silicon, silver, fluorine co-modified hydroxyapatite (Si-Ag-F-HA) nano-biofilms was deposited on CP-Ti through electrodeposition. Ag was incorporated into HA coating to improve the antimicrobial properties. Si was added as a second binary element to offset the potential cytotoxicity of Ag. The as-prepared coatings were examined by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) tests. Results highlight that F-, Si4+ and Ag+ could be evenly incorporated into the Si-Ag-F-HA coating. The results indicate that the Si-Ag-F-HA coatings take the morphology of nanoscale- villous-like, the composite coating becomes more compact. The composite coatings were found to be bioactive, based on the promotion of additional apatite onto the Si-Ag-F-HA coating surface from SBF. Potentiodynamic polarisation tests revealed that the corrosion resistance increased after Si-Ag-F-HA coating. The release of Si and Ag ions from Si-Ag-F-HA coatings shows sustained release kinetics without burst release, which reached a near steady state afterwards, thereby revealing long-term sustainable release. FTIR and ICP-MS provide a rapid and effective method for the development of new antibacterial hard tissue repair materials. © 2018, Peking University Press. All right reserved