Development of Glucosamine Sulfate Certified Reference Material

研制了硫酸氨基葡萄糖标准样品。以盐酸氨基葡萄糖为原料,制备高纯硫酸氨基葡萄糖,采用红外光谱(Ir)、高分辨质谱和核磁共振谱(nMr)进行结构确证。样品分装成200瓶样品后,采用高效液相色谱–蒸发光散射法进行均匀性检验、稳定性检验和定值分析。从样品中随机抽取15瓶进行均匀性检验,结果表明在95%的置信区间范围内样品均匀性良好。按照25℃长期试验稳定性(12个月)进行稳定性考察,结果表明在考察期间内样品稳定性良好。标准样品经国内8家具有分析资质的实验室进行协同定值,硫酸氨基葡萄糖标准样品定值结果为99.84%,相对扩展不确定度为0.18%(k=1.96)。该标准样品达到国家标准样品的技术要求,可用于有关硫酸氨基葡萄糖的分析方法校正和质量控制。Glucosamine sulfate certified reference material was developed.Glucosamine sulfate was made from the glucosamine hydrochloride.The structure of glucosamine sulfate certified reference material was comfirmed by infrared spectroscopy(IR), high resolution mass spectral and nuclear magnetic resonance(NMR).The sample was divided into 200 bottles, the homogeneity, stability testing and quantitative analysis were carried out by high performance liquid chromatography with evaporative light-scattering detector(HPLC–ELSD).According to analysis procedure of homogeneity under the confidence interval of 95%, 15 bottles of sample were randomly taken from 200 bottles, and the results were validated by F-test statistical methods.The stability inspection was carried on the long-term(12 months), and the results indicated that the period for glucosamine sulfate of storage was 12 months at 25℃.A cooperative certification was conducted with eight qualified laboratories.The certified purity value of the reference material of glucosamine sulfate was 99.84% with a relative expaned uncertainty of 0.18%(k=1.96).The reference material can conform to the technical requirement of the certified reference material.The material was intended for use in the method validation and quality control regarding glucosamine sulfate.厦门市科技计划项目(3502Z20130004); 海洋生物技术产业化中试技术研发公共服务平台(12PZP001SF10); 广东海洋经济发展区域示范项目(GD2012–D01–001

儿童附件扭转的CT特征与临床对照分析

目的分析总结儿童附件扭转（AT）的临床特征及CT表现，提高儿童AT的诊断准确率。方法回顾性分析经手术病理证实，具有完整临床及CT资料的26例（27灶）儿童AT病例，归纳总结儿童AT的影像特征及病理学基础。根据有无临床症状，分析各种影像征象的阳性率。结果26例AT患者年龄为1 d至12岁，平均5.5岁。20例（20/26）以下腹痛为首诊症状。26例中有15例位于右侧（15/26），10例位于左侧（10/26），位于双侧者1例（1/26）。25灶（25/27）为完全性扭转，2灶（2/27，7.4%）为不全性扭转。19灶（19/27）合并占位性病变，8灶（8/27）为单纯附件扭转。病灶范围3.4~14.0 cm，平均5.9 cm。12灶（12/27）患侧卵巢体积增大。14灶（14/27）病灶内发现钙化。20灶（20/27）病灶周围发现4~12 mm小囊状结构。增强扫描26灶（26/27）病灶轻度强化（<20 HU）。漩涡征阳性11灶（11/27），子宫向患侧移位12灶（12/27），腹水7灶（7/27），盆腔脂肪间隙模糊11灶（11/27）。CT诊断符合率为88.9%。结论胎儿期卵巢囊肿出现囊壁钙化应考虑AT可能。畸胎瘤及卵巢囊肿是儿童AT的危险因素。不对称卵巢体积增大、周围小滤泡是儿童AT特异性较高的CT征象；若同时出现漩涡征、子宫移位、腹水、周围脂肪间隙模糊、畸胎瘤（或囊肿）壁增厚时，应高度怀疑儿童AT的可能

昆虫杆状病毒表达载体系统在疫苗研究中的应用进展

昆虫杆状病毒表达载体系统(Baculovirus expression vector system,BEVS)已成功应用于多种蛋白的表达,并为疫苗开发提供了充足的原材料。相比其他表达系统,BEVS具有许多优势:杆状病毒专一寄生于无脊椎动物,安全性高;重组蛋白表达水平高;可对重组蛋白进行正确折叠和翻译后修饰,获得具有生物活性的蛋白;适应于多基因表达如病毒样颗粒(Virus-like particle)的复杂设计;适用于大规模无血清培养等。为了更好地理解BEVS在疫苗研究中的应用前景,文中将从BEVS的发展及其在疫苗研究中的应用等方面进行综述。国家自然科学基金(Nos.U1705283,31670935,81701637)资助~

Analysis on the land use and cover change in Tianjin Binhai New Area based on the remote sensing

采用全国土地分类系统,基于lAndSAT卫星TM遥感影像与天津滨海新区1:50,000地形图,进行相应数据处理,编制出2000年、2005年、2010年三期滨海新区土地利用/覆盖分类图,并运用土地利用转移矩阵对近10年来天津滨海新区土地利用/覆盖变化进行定量分析。在此基础上,进一步分析了经济、人口等驱动因子对土地利用/土地覆盖的影响。结果表明:十年间,滨海新区的建筑用地面积增加显著,而绿地、湿地、滩涂等高生态价值的土地向建筑用地、未利用地等开发建设用地大幅度转化,后五年变化尤为显著。This paper carries out quantitative analysis on the land use/cover(LU/C) change of Tianjin Binhai New Area in recent 10 years through using land use transition matrix from the three-stage LU/C classification maps of 2000, 2005 and 2010 drafted by means of the National Land Classification System of China based on Landsat TM satellite remote sensing image and the Tianjin Binhai New Area 1:50 000 relief maps.On this basis, the impact of such driving factors as the economy and population on LU/C is further analyzed.The results show that the area of the building land in Binhai New Area has increased significantly over the ten years, and the greenland, wetland, and shoals of high ecological value have been dramatically transformed into the building land and unused land for the development and construction, and the change is more significant in the later five years

水痘-带状疱疹病毒糖蛋白gE在昆虫细胞中的表达鉴定及其晶体培养

目的利用杆状病毒-昆虫表达系统建立纯化水痘带状疱疹病毒(VZV)包膜糖蛋白gE的方法,筛选gE蛋白的晶体培养条件,以期用于结构解析。方法将VZV糖蛋白gE基因序列克隆至杆状表达载体pAcgp67B载体中,利用High FiveTM昆虫细胞表达gE蛋白并进行TALON亲和层析纯化;利用WAVE生物波浪反应器建立含硒代甲硫氨酸的gE蛋白方法,以便在晶体结构解析中利用单波长或多波长异常散射进行相位解析;通过分子排阻色谱、分析超离,差示扫描量热法和酶联免疫吸附试验等分析gE蛋白的理化性质;利用结晶试剂盒对gE498和gE354蛋白的结晶条件进行初筛。结果获得的gE498和gE354蛋白纯度约为90%、产量为8～10 mg/L。理化分析显示两种gE蛋白在溶液中主要以均一稳定的单体形式存在,并呈现出良好的反应原性。在gE354晶体初筛中获得3个结晶条件:CrystalH3、PEGH12和IndexB10。结论建立了VZV糖蛋白gE表达和纯化方法,制备的蛋白纯度高,且具有反应原性,并筛选出CrystalH3、PEGH12和IndexB10这3个结晶条件,为gE糖蛋白的结构与功能研究及新型疫苗开发奠定了基础。国家自然科学基金项目(No.81871648);;\n新药创制专项项目(No.2018ZX09711003-005

Numerical Simulation of Fluid Transport along Parallel Vanes for Vane Type Propellant Tanks

Fuel tanks are a core component in satellites that manage the propellant. This study numerically analyzed the fluid transport with parallel guide vanes in a vane type surface tension tank. Flow3D was used to simulate fluid transport in microgravity in a scale model with 15% liquid filling rate with comparisons to experimental data. Then, the simulations were used to compare the leading edge climbing speed for various working conditions and the liquid volume below a specified cross section for various liquid filling rates, various numbers of vanes. The results show that the liquid climbing process on the guide plate in the plate tank can be divided into the liquid level repositioning stage after the gravitational force is suddenly removed and the stable fluid transport stage. Throughout the entire capillary flow stage, the liquid leading edge climbing rate is not related to the filling rate of the number of vanes and the fluid transport efficiency of a single guide vane is independent of the total number of vanes

中国科学院力学研究所国家微重力实验室;

研究了微重力板式贮箱三维平衡界面的有关问题,进行了相应的数值模拟和落塔实验验证。通过流体分析软件Surface Evolver模拟了板式贮箱在零重力下不同充液率时气液平衡界面,分析得到了该种板式贮箱最大排挤量。在微重力情况下,通过Surface Evolver模拟研究了不同重力加速度方向对气液平衡界面的影响,得到当充液率小于5%,气液平衡界面不受重力加速度影响,液体集中在液口位置,当充液率较大时,液体沿加速度方向有一定偏移。针对微重力模拟结果,采用酒精作为实验介质,微重力水平10~(-3)g进行3.6s微重力落塔实验,将实验结果与模拟结果进行比较,证明了模拟方法的正确性,同时得到了在正向加速度..

a solution to support tibetan coded character set extension a/b in linux system

国内藏文软件开发普遍使用的是基于垂直预组合字符的实现方案,但是缺乏统一的编码标准.藏文编码字符集扩充集的推出,对于国内藏文软件的标准化、国际化具有重要意义.本文通过分析ISO/IEC 10646藏文编码字符集基本集、藏文编码字符集扩充集国家标准,区分它们描述字丁的差异,分析由编码方案所导致的实现上的关键问题.最后,针对藏文扩充集B的特殊性,提出并实现了基于Linux国际化架构下支持藏文扩充集标准的解决方案.中国中文信息学