Polynomial Equations

計畫編號：NSC89-2115-M032-013研究期間：200008~200107研究經費：280,000行政院國家科學委員

[[alternative]]Study on Invariant Rings

計畫編號：NSC94-2115-M032-004研究期間：200508~200607研究經費：526,000[[sponsorship]]行政院國家科學委員

Computation of the index of some meromorphic functions of degree 3 on tori

doctoral thesi

Electrochemical Construction of Ordered NanoHA Coating and in situ EIS Study on Titanium/Cell Interface

医用金属钛及其合金的表面活性改性及材料的生物学响应是生物材料研究领域的焦点之一。针对当前国内外在钛表面的羟基磷灰石（HA）涂覆、HA涂层组分和结构控制、HA涂层形成机理及主要影响因素以及生物材料与细胞的相互作用机理等关键技术和科学问题，本论文工作旨在从仿生学角度进一步发展温和的电化学沉积技术在医用钛金属表面构筑具有优良生物性能和力学性能的有序结构HA涂层，探明高生物活性涂层的形成机理及规律性；发展电化学交流阻抗谱（EIS）技术用于原位检测材料/细胞相互作用及其存在的电化学本质联系，以探明生物材料与细胞相互作用过程的界面结构。本论文主要研究内容有：a)侧重采用低浓度体系的电化学沉积技术制备均匀纳...The surface modification for bioproperties and biological response of titanium are one of the most focal studies in biomaterials field. The emphases of this thesis research are put on some scientific and technical key problems, such as the novel coating techniques of hydroxyapatite (HA), the control of composition and structure of HA coating, the mechanism and main influence factors of HA coating ...学位：理学博士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：B20022501

Single Cell Electrochemistry

采用电化学的方法检测单细胞水平的生命现象（包括氧化应激和胞吐作用等）可为相关疾病的病理研究及诊断治疗等提供重要的理论和实验依据。然而，由于单细胞电化学检测的技术细节繁杂，对操作者技能的要求很高等原因，目前国际上只有少数几个小组（Wightman小组、Ewing小组、Amatore小组等）具有完备的实验系统。本工作的主要内容是搭建适用于单活细胞电化学检测的系统设备，构建细胞模型并在其基础上结合具体的生物学问题进行实验以获得技术应用方面的突破。 在中法国际联合实验室XiamENS的工作框架下，我们与Amatore教授实验室合作开展了单细胞水平MG63成骨肉瘤细胞的氧化应激释放物种的检测工作，并且...In the 1990s, the electrochemical technique was developed to monitor the cellular secretions at single living cell level, owing to its advantages of excellent sensibility, high temporal resolution, direct detection, etc. The electrochemical studies on living cell activities, such as oxidative stress and exocytosis provide both theoretical support and experimental inquiries to our understanding of ...学位：博士后院系专业：生命科学学院生物学系_细胞生物学学号：BH1700023

金融业混业经营:中国的选择模式

分业经营和混业经营是金融业的两种经营模式。自20世纪90年代以来,实行金融分业制度的国家也大多转向了混业经营制度。在国际经济金融一体化的今天,我国金融企业将直接面临实行混业经营的金融巨无霸的挑战。但目前我国金融市场发育尚不完善,金融法律体系也不健全,金融监管水平也比较低下,社会信用观念比较淡薄,以及我国经济体制改革是渐进式的漫长过程,所以必须结合我国实际,走一条渐进式的金融混业经营道路

Effect of HJJB Compound on Insulin Signal Transduction Link of Non-alcoholic Steatohepatitis Rats

目的观察红景天苷、姜黄素、绞股蓝总苷、白术多糖(HJJB)复方对非酒精性脂肪性肝炎(NASH)大鼠胰岛素信号转导环节的干预作用。方法采用高脂饮食; 14周诱导的大鼠胰岛素抵抗NASH模型。在造模第9周起,随机分为模型组、西药组(罗格列酮,0.4; mg/kg)和中药组(HJJB)。干预6周后,观察肝组织病理变化(HE染色),检测肝组织TG含量、ALT活性、血清空腹胰岛素(FINS)含量、空; 腹血糖(FBG)含量、胰岛素抵抗指数(HOMA-IR),检测肝组织胰岛素受体底物1 (IRS1 )、磷酸化IRS1 (pIRS1; )、磷脂酰肌醇-3-激酶(PI3K)、磷酸化PI3K(pPI3K)、蛋白激酵B(PKB)、磷酸化PKB(pPKB)蛋白含量;检测肝组织IRS1、; PI3K、PKB mRNA水平。结果与正常组比较,模型组出现肝细胞脂肪变性, TG、ALT、FINS、FBG 及 HOMA-IR 升高(P; <0. 01), IRS1、plRS1、PI3K、pPI3K、PKB、pPKB 蛋白及 IRS1、 PI3K、PKB mRNA降低(P; <0.01)。与模型组比较,中药组和西药组上述病理改变明显减轻,血清TG、ALT、 FINS、FBG 及 HOMA-IR; 含量降低(P<0.05)。中药组 IRS1、pIRS1、PI3K、pPI3K、PKB、pPKB 蛋白及 IRS1、 PI3K、PKB; mRNA水平较模型组及西药组升高(P <0.01,P <0.05),TG及ALT较西药组降低(P <0. 01; )。结论HJJB复方可上调NASH大鼠肝脏IRS1基因表达和蛋白含量,改善PI3K/PKB信号通路。Objective To observe the intervention effect of HJJB; compound(salidroside, curcumin, gypenosides and atractylodes; polysaccharides) on insulin signal transduction link of non-alcoholic; steatohepatitis (NASH) rats. Methods SD male rats were induced by; high-fat diet for 14 weeks for insulin resistance NASH model. From the; ninth week, the rats were divided into the model group, the Western; medicine(WM) group (rosiglitazone, 0. 4 mg/kg) and the Chinese medicine; (CM)group (HJJB) at random .Six weeks after medication, liver pathology; (HE staining), hepatic TG content, serum ALT activity, serum fasting; insulin (FINS), serum fasting blood glucose( FBG), insulin resistance; index (HOMA-IR) were observed. Protein content of hepatic insulin; receptor substrate insulin receptor substrate 1 (IRS1), phosphorylation; of IRS1 (pIRS1),phosphatidylinositol-3 kinase (PI3K) , phosphorylation; of PI3K(pPI3K), protein kinase B (PKB) and phosphorylation of PKB (pPKB); were detected. mRNA expression of hepatic IRS1,PI3K, PKB were also; detected. Results Significant hepatic steatosis were observed in the; model group. TG, ALT, FINS, FBG and HOMA-IR of model group were higher; than those of the normal group(P < 0. 01). Hepatic IRS1,pIRS1 ,; PI3K,pPI3K, PKB, pPKB protein expression level and IRS1 , PI3K,PKB mRNA; level were lower than those of the normal group (P <0. 01). Hepatic; pathological changes in the CM group and WB the group were meliorated,; ALT, FINS, FBG, HOMA-IR and TG of the CM group and the WM group were; lower than those of the model group(P <0. 05). Hepatic; IRS1,pIRS1,PI3K,pPI3K, PKB, pPKB protein expression level and IRS1,; PI3K, PKB mRNA of the CM group were higher than those of the model group; and the WM group(P <0. 01,P <0. 05),ALT and TG of the CM group were; lower than those of the model group (P <0. 01 ). Conclusion HJJB; Compound can significantly increase hepatic IRS1 gene expression and; protein content of fatty liver in rat, and then improve the PI3K/PKB; signal pathways.国家自然科学基金资助项目; 浙江省自然科学基金资助项目; 浙江省中医药科技计划项