157 research outputs found

    Principal - Agent System of Gas Station Managers in Station Network Management

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    纵观100年的加油站发展史,汽车工业的快速发展无疑是决定性力量,而顾客对便利性的追求始终是推动行业变革的主要动力。特别是近20多年来,欧美等发达国家和地区的加油站行业技术设备升级加快,逐步实现了精细化管理。随着便利店、超市运营商等纷纷加入,市场竞争更趋激烈,推动着加油站行业不断向前发展。加油站经营者都希望能预见未来的发展趋势,并适时做出积极应对。特别是经营大规模加油站网络,油站经理的委托代体系是油站管理的核心工作。油站经理面临着公司市场营销、油品与非油品的供应链管理、安全管控与内控管理的核心节点等诸多方面的管理难题,同时对构建公司整体的核心竞争力中起着极为关键的作为。 本论文利用相关管理理论...Throughout 100 years history of the gas station, rapid development of the auto industry is undoubtedly decisive force, but requirement for the convenience from customers is the main power to promote industry reform all the time. Especially in recent 20 years, upgrade acceleration of gas station industry technology and equipment in Europe, America and other developed countries, gradually realizes f...学位:工商管理硕士院系专业:管理学院_工商管理硕士(工商管理硕士)学号:1792011115094

    厦门大学图书馆馆藏书目信息API开发实例及其应用

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    Web2.0环境中OpenAPI成为了越来越多网站开放数据服务的选择。本文以厦门大学图书馆馆藏书目信息API的开发为实例,探索了图书馆开放数据服务的方式,并介绍了以此API为基础的豆瓣网Mashup应用,说明了其的实用价值

    AMP依赖的蛋白激酶及PI3K/Akt信号途径在颈动脉狭窄形成过程中的变化

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    目的 观察AMP依赖的蛋白激酶(AMPK)及PI3K/Akt信号途径在颈动脉狭窄形成过程中的变化。方法 取SD大鼠经高脂喂养1个月后行颈总动脉球囊损伤术作为模型组(n=24),继续喂养14 d后取损伤侧颈总动脉,行HE染色观测血管内皮损伤及内膜增生情况,应用荧光定量PCR法检测AMPK及PI3K/Akt mRNA水平,用Western印迹检测p-AMPK、PI3K、p-Akt蛋白水平,另取8只正常SD大鼠作为假手术组。结果 模型组大鼠血管内膜增生明显、管壁变厚;AMPK/PI3K/Akt mRNA及蛋白水平较假手术组显著下降(P〈0.01)。结论 AMPK/PI3K/Akt在颈动脉狭窄的病理生理进程中起到一定的作用。福州市科技计划项目(No.2012-S-155-1

    Effect of Lunei Xiaoyu Decoction on Vascular Endothelial AMPK/eNOS Signal in Balloon Injury Model of Common Carotid Artery in Rats

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    目的观察颅内消瘀汤对大鼠颈动脉球囊损伤后血管内皮及AMPK、eNOS表达的影响,探讨其可能的作用机制。方法 32只SD大鼠随机分为假手术组、模型组、颅内消瘀汤低剂量(临床等效量)组和颅内消瘀汤高剂量(临床2倍量)组,除假手术组大鼠仅切开皮肤,其余大鼠均行颈总动脉球囊损伤术以成模。成模第4天灌胃给药,模型组及假手术组给予等体积生理盐水,连续给药14 d后取损伤侧颈总动脉,行HE染色观测血管内皮损伤及内膜增生情况,用免疫组化染色检测eNOS表达水平,并用Western blot检测总AMPK、eNOS蛋白水平。结果颈总动脉球囊损伤模型组大鼠血管内膜增生明显、管壁变厚,颅内消瘀汤低、高剂量组大鼠经治疗后损伤血管增生较模型组轻、管壁较薄;e NOS免疫组化染色:模型组阳性细胞较少,经颅内消瘀汤干预后,低、高剂量2组均有不同程度的表达,与模型组比较差异有统计学意义(P<0.01);Western blot检测结果显示模型组总AMPK、eNOS蛋白表达下降,经颅内消瘀汤干预后,均有不同程度增加,与模型组比较差异有统计学意义(P<0.05或P<0.01)。结论颅内消瘀汤可通过上调AMPK/eNOS信号表达,保护大鼠颈动脉球囊损伤血管内皮的作用。OBJECTIVE To observe the effect of Lunei Xiaoyu Decoction on vascular endothelial AMPK, eNOS expression in ballon injury model of common carotid artery in rats, and to explore its possible mechanism. METHODS Thirty two SD rats were randomly divided into sham operation group, model group, low dose of Lunei Xiaoyu Decoction(dose in clinical equivalent) group and high dose of Lunei Xiaoyu Decoction(2 times the amount of clinical) group, except the sham operation group rats only skin incision, the other rats underwent carotid artery balloon injury surgery. On the 4th day after modeling, the rats were administrated orally for 14 d, model group and sham operation group were given equal volume of physiological saline. Then all the rats were killed, HE staining observation of vascular endothelial injury and intimal hyperplasia, immunohistochemistry staining was used to detect the expression level of e NOS, and Western-blot was used to detect the expression level of AMPK and eNOS. RESULTS The endometrial hyperplasia and thickness were obvious after carotid artery balloon injury in rats; and after Lunei Xiaoyu Decoction treatment, the rats' endometrial hyperplasia and thickness were improved. The expression of AMPKand e NOS was significantly higher in Lunei Xiaoyu Decoction groups compared with the model group(P<0.05 or P<0.01). CONCLUSION Lunei Xiaoyu Decoction can upregulate the expression of AMPK/e NOS signal, in order to protecting vascular endothelial after balloon injured carotid artery in rats.福州市科技计划项目(2012-S-155-1

    制度变革、非正式制度与会计审计行为——中国会计学会英文期刊China Journal of Accounting Studies(CJAS)2018年第二次学术研讨会会议综述

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    2018年10月26日,由中国会计学会、中国会计学会对外交流专业委员会主办,厦门大学会计学系承办的中国会计学会对外学术交流专业委员会学术年会暨China Journal of Accounting Studies 2018年第二次学术研讨会在厦门大学隆重举行。来自复旦大学、中山大学、中国人民大学、西安交通大学、厦门大学、重庆大学、湖南大学、暨南大学、香港中文大学、香港城市大学、美国纽约州立大学石溪分校、澳大利亚新南威尔士大学、对外经贸大学、中央财经大学等三十多所境内外高等院校和研究机构的90余名专家学者参加了本次学术研讨会,围绕会议主题\"制国家自然科学基金重大项目课题“制度变革、非正式制度因素与会计审计行为研究”(71790602);;教育部人文社科基地重大项目“文化影响、会计信息质量与审计行为”(16JJD790032)资

    分子生物学技术在水产养殖中的应用

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    福建省自然科学基金资助项目!C97015

    Effect of the Construction of Green Belts on the Attenuation of Traffic Noise along the Urban Trunk Roads in Xiamen City

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    通过对厦门市主干道绿化带种类结构调查以及噪声测定等,分析了厦门市主干道绿化带结构及其减噪效果。结果表明,厦门市主干道绿化带可分为4种结构:单一乔木型、乔木+疏灌木/绿篱型、乔木+密灌木型以及乔木+小乔木+灌木/绿篱型,带宽多在4~10M。厦门市主干道绿化带总体减噪能力为0.93~12.96db,绿化带对交通噪声超标治理率达70%。绿化带减噪能力y(db)与带宽X(M)呈显著的线性关系:y=1.2251X+0.2416(r2=0.8603);绿化带的附加衰减与总衰减亦呈显著正相关:y=0.4535X+0.2698(r2=0.9242),噪声的附加衰减主要受绿化带结构的影响,上述四种结构对噪声附加衰减平均值分别达0.93、2.25、4.43和6.72db。绿化带的宽度和结构均是影响其减噪效果的关键因素。Through site investigation and noise monitoring, the effects of the construction of green belts along the urban trunk roads in Xiamen Island on the attenuation of noise has been studied.The results show that the construction of green belts along the trunk road can be divided into four kinds of types as bellows: type I, single arbor; type II, arbor + shrub; type III, arbor + sub-arbor and type Ⅳ, arbor + sub-arbor + shrub.Their width varied among 4--10 m.The noise-reducing ability (y, dB) of the green belts varied from 0.93 to 12.96 dB with various belt widths (x, m), and can be expressed as following: y=1.2251x+0.2416.Meanwhile the additive attenuation of traffic noise (y, dB) presents a closely linear relationship with the total noise-reducing ability (x, dB) as following: y=0.4535x+0.2698 (R2=0.9242).Meanwhile, the additive attenuation of traffic noise is main affected by the constructions of green belts, which reached 0.93, 2.25, 4.43 and 6.72 dB, respectively for the above four kinds of construction types.It shows that the width and construction of green belts play the same important role to reduce the traffic noise

    Analysis of genetic variability of Castanopsis hystrix by rbcL gene sequences

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    通过PCR扩增得到10个红锥品系的rbcL基因全序列,均为1.513 kb.核苷酸序列比对结果表明,在256-1 455 bp区间10个红锥品系共有16处碱基发生变异,变异率为1%,其中在306 bp和634 bp处10个红锥品系较同属对照castanopsis lucida均发生相同的变异,可以看作属内进化信息碱基.对表达的氨基酸序列进行比对分析,发现16处碱基变异共引起8处氨基酸序列变异.The rbcL gene sequences of 10 assassin of Castanopsis lucida were analyzed by PCR,and sixteen mutations in 256-1 455 bp were found,the mutation ratio was 1 %.Compared to Castanopsis lucida,between 306 bp and 634 bp,they had two characteristic mutations of their own,so it was different from Castanopsis lucida.Compared to Castanopsis lucida,8 amino acid variations were found,caused by 16 point mutations.广西林业科学研究院国家林业局中南速生材繁育重点实验室开放基金课题;; 兰州理工大学博士基金(SB08200602);; 甘肃省教育厅研究生导师基金( 0703-11)

    Expression Analysis of a Stress Repressed Gene OsDSR4 from DUF966 Family and Generation of OsDSR4- overexpressing Transgenic Rice(Oryza sativa L. ssp. japonica)

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    OSdSr4基因是duf966基因家族中的一个未知功能基因,目前其生物学功能尚不清楚。本研究生物信息学分析显示,OSdSr4基因CdnA全长2 167 bP,包含一个1 149 bP的开放阅读框(Orf),编码382个氨基酸,推测的蛋白中包含一个高度保守的duf966结构域;表达模式分析表明,OSdSr4主要在水稻(OryzA SATIVA l.SSP.JAPOnICA)的茎节间和叶片中表达,干旱、高盐和低温等非生物胁迫明显抑制了OSd Sr4的表达,而脱落酸(AbSCISIC ACId,AbA)则显著诱导了它的表达;利用重叠延伸PCr方法成功克隆了OSdSr4,并将其转化进水稻中,获得了32株超表达转基因植株。分子鉴定结果表明,该基因已被整合进水稻基因组中,并在部分转基因植株中实现了超量表达。本实验为进一步开展OSdSr4基因的生物学功能研究提供了基础资料。OsDSR4 is a gene of unkown function in DUF966 gene family,and the function of DUF966 family genes have not been reported until now.In this study,the bioinformatic analysis showed that the cDNA of OsDSR4 had 2 167 bp containing an open reading frame(ORF) of 1 149 bp,and it encoded a putative protein of 372 amino acids with a highly conserved DUF966 domain.The gene expression profile analysis indicated that OsDSR4 was expressed mainly in internode and leaf blade of rice(Oryza sativa L.),and it was repressed markedly by drought,salt and cold stresses,and induced significantly by abscisic acid(ABA).OsDSR4 was cloned using overlap extension PCR,and the fusion construct containing OsDSR4 was introduced into rice(Oryza sativa L.ssp.japonica) by Agrobacterium- mediated transformation method.Thirty- two OsDSR4- overexpressing transgenic plants were obtained and identified by PCR and qRT-PCR,which was demonstated that OsDSR4 had been integrated into rice genome and was overexpressed in some positive transgenic plants.These results establish the foundation for further study of the precise function of OsDSR4.国家重点基础研究发展计划(973)前期研究专项(No.2012CB126312
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