Source apportionment of water-soluble ions in spring TSP of Pengjia Islet, Taiwan

于2010年春季,在台湾省北部东海海域彭佳屿岛采集了60个总悬浮颗粒(TSP)样品,用离子色谱测定了8种水溶性离子(Na~+、Mg~(2+)、; Ca~(2+)、K~+、NH_4~+、Cl~-、SO_4~(2-)和NO_3~-)浓度.结合HYSPLIT气团后向轨迹模型、离子化学计量学关系、; 离子相关性分析和主成分分析,探讨了彭佳屿岛春季TSP中水溶性离子的主要来源.结果表明:彭佳屿岛春季TSP中水溶性离子质量浓度顺序依次为SO_4~; (2-)(7.704.53)mug/m~3> Cl~-(6.17 3.85)mug/m~3>Na~+(4.59 ; 2.28)mug/m~3>NO_3~-(4.24 3.07)mug/m~3>NH_4~+(1.53 ; 1.20)mug/m~3>Ca~(2+)(0.95 1.47)mug/m~3>Mg~(2+)(0.59 ; 0.30)mug/m~3>K~+(0.310.17)mug/m~3.其中Cl~-、Na~+和Mg~(2+)全部来源于海洋源;K~+来源较多,除了; 海洋源外,煤和生物质燃烧以及陆源矿物等都可能是彭佳屿岛春季TSP中K~+的来源;60%的Ca~(2+)来源于当地的建筑粉尘;77%的SO_4~(; 2-)源于煤和生物质燃烧释放;NO_3~-主要源于汽车尾气、煤和生物质燃烧等释放;NH_4~+主要来源于二次气溶胶和生物质燃烧等.通过整合本研究; 与前人的观测,发现在春季从中国东北至西北太平洋开阔海域,TSP中Cl~-、Na~+和Mg~(2+)的浓度在空间上呈现逐渐升高趋势,而Ca~(2+; )、K~+、NH_4~+、SO_4~(2-)和NO_3~-的浓度在空间上呈现逐渐下降趋势,显示了TSP中不同水溶性离子在海洋大气传输过程中的空间; 变化特征.In spring of 2010, sixty total suspended particulate (TSP) samples we; collected in Pengjia Islet, northern sea of Taiwan, China. The; concentrations of water-soluble ions (Na~+, Mg~(2+), Ca~(2+), K~+,; NH_4~+, Cl~-, SO_4~(2-) and NO_3~-) in the TSP samples were extracted; and then determined using an Ion Chromatography. Using these data, we; investigated the sources of water-soluble ions by a multiple-technique; analysis combining with the HYSPLIT model, ion stoichiometry,; correlation analysis of water-soluble ions and primary component; analysis. According to a decreasing order in the average mass; concentration, these ions ranked as SO_4~(2-)(7.704.53)mug/m~3,; Cl~-(6.173.85)mug/m~3, Na~+(4.592.28)mug/m~3, NO_3~-(4.24 3.07)mug/m~3,; NH_4~+(1.531.20)mug/m~3, Ca~(2+)(0.951.47)mug/m~3,; Mg~(2+)(0.590.30)mug/m~3 and K~+(0.310.17)mug/m~3. The sea water was; identified as the dominant source for Cl~-, Na~+ and Mg~(2+). Besides; the contribution from the sea salt, the K~+ had other sources, such as; coal and biomass burning, and mineral etc. 60% Ca~(2+) came from the; local construction dust, while 77% SO_4~(2-) originated from coal and; biomass burning. The NO_3~- dominantly sourced from the vehicle exhaust,; coal and biomass burning, while NH_4~+ totally originated from the; second inorganic aerosol and biomass burning. By combining our results; with previous findings, in spring, the concentrations of Cl~-, Na~+ and; Mg~(2+) in TSP increased gradually from the Northeast of China to the; Northwest Pacific Ocean; on the contrary, the Ca~(2+), K~+, NH_4~+,; SO_4~(2-) and NO_3~- in TSP decreased gradually. The spatial; characteristics of different water-soluble ions in TSP were associated; with their long-range transport in the marine boundary layer.国家973计划项目; 国家自然科学基金项目台湾海峡联合基金资助项目; 东华理工大学校科技创新团队科研启动基金项目; 江西省教育厅科技项目;; 东华理工大学博士科研启动基金项

On the Reconstruction of the Ownership System of Aquatic Wild Animals

我国现行立法对水生野生动物所有权规定,存在着内涵不明确,外延不周延,立法规范与实践相背离等方面的问题。国外的立法实践可为我国的立法完善提供参考思路;突破传统的物权客体理论需要理念革新;明确界定水生野生动物的所有权具有重要的制度价值。我国对水生野生动物的所有权结构应具有多元性;权利主体的权利行使方式应有所限制。There are many problems on current provisions of ownership system of aquatic wild animals,for its ambiguous conception and range,the conflict between legislation and practice,etc.The alien legislation examples provide some useful information for us to improve our legislation.We need to change our traditional perspective of the object theory of ownership.The definite confines of the ownership of aquatic wild animals consists of some special system values.We should construct multiform ownership system for aquatic wild animals,and the exertion methods of the ownership should be restricted.农业部课题《水生野生动植物保护立法研究与建议》(RYB0801)阶段性研究成

Acoustic Emission Warning Signals Change Law Research during Deformation and Failure of Gassy Coal Rock

为提高矿井瓦斯突出灾害预测准确率,基于力学、声学理论,采用实验室试验方法,以标准煤试样为研究对象,对含瓦斯煤岩变形破裂过程声发射(AE)预警声信号变化规律进行研究。试验结果表明:围岩应力增大,煤岩破裂由低围岩应力的脆性破坏逐渐过渡到高围岩应力状态的塑性破坏,可根据声发射高幅信号提前预测煤岩破裂时刻。瓦斯的力学和非力学作用对煤岩体力学性质和变形特性的影响均随瓦斯压力的增加而变大。瓦斯压力越大,则煤岩破裂进程加快,积累的声发射能量迅速释放,高值振铃计数声发射信号覆盖时间区段变长,声发射信号能及时反映含瓦斯煤岩的不稳定性

退行性颈椎不稳运动降低椎动脉与小脑灌注血流动力的多模态MRI初期研究

目的采用椎动脉MR流速测定(2D-PC Cine)和动脉自选标记(ASL)成像研究退行性颈椎不稳运动对椎动脉和小脑灌注血流速度的影响。方法选取符合纳入标准的椎动脉迂曲并颈椎不稳者8例(观察A组),单纯椎动脉迂曲者6例(观察B组),椎动脉正常者6例(对照组)。在仰卧位分别行颈椎中立位、左旋转45°和右旋转45°椎动脉2D-PC Cine及小脑ASL成像。测量左侧与右侧椎动脉平均血流速度、血流速度落差,以及小脑灌注血流速度。结果无论是左侧还是右侧椎动脉,观察A组、观察B组、对照组颈椎不同体位下平均血流速度差异无统计学意义(P>0.05);但3组间颈椎不同体位下血流速度落差比较差异有统计学意义(P0.05);颈椎左旋转45°与右旋转45°时,3组小脑灌注血流速度差异有统计学意义(P<0.05),且观察A组小脑灌注血流速度明显低于观察B组与对照组。结论退行性颈椎不稳运动可引起椎动脉血流速度落差增大,降低小脑灌注动力。漳州市科技计划项目(Z07019

Experimental study on effect of Changrun Formula in regulating expression of AQP3 and AQP9 in colon mucosa of functional constipation rats

目的:观察肠润方对功能性便秘模型大鼠结肠黏膜AQP3、AQP9表达的影响,探究其调控AQP3、AQP9表达的分子机制。方法:60只SD大鼠随机分为空白组、模型组、阳性对照组(麻仁丸组)、肠润方组、肠润方联合P38MAPK抑制剂SB203580组。用复方地芬诺酯制造大鼠便秘模型,采用免疫组织化学及实时荧光定量PCR(q RT-PCR)检测大鼠便秘模型近端及远端结肠黏膜AQP3、AQP9的表达;Western Blot检测信号传导通路相关分子P38MAPK及P-P38MAPK的表达。结果:造模成功后,模型组近端结肠黏膜AQP3表达较空白组明显上升,而远端结肠黏膜AQP9表达较空白组明显下降(t值分别为3.148和7.069,P值均0.05)。肠润方联合P38MAPK抑制剂SB203580治疗后,近端结肠黏膜AQP3 m RNA水平显著上升(t=5.922,P0.05). The level of AQP3 m RNA in proximal colonic mucosa was significantly increased(t=5.922, P<0.01), and AQP9 m RNA was significantly decreased in distal colonic mucosa(t=4.038, P<0.01); The relative expression of P-P38/P38 protein was significantly decreased(t=19.419, P<0.01). Conclusion: The therapeutic action ofChangrun Formula on functional constipation rat models might through inhibiting the expression of AQP3 in proximal colon, and accelerating on expression of AQP9 in distal colon, and the regulating mechanism might relate with the inhibition on P38 MAPK and AKT phosphorylation.福建省自然科学基金项目(No.2012D043)~

Expression Analysis of a Stress Repressed Gene OsDSR4 from DUF966 Family and Generation of OsDSR4- overexpressing Transgenic Rice(Oryza sativa L. ssp. japonica)

OSdSr4基因是duf966基因家族中的一个未知功能基因,目前其生物学功能尚不清楚。本研究生物信息学分析显示,OSdSr4基因CdnA全长2 167 bP,包含一个1 149 bP的开放阅读框(Orf),编码382个氨基酸,推测的蛋白中包含一个高度保守的duf966结构域;表达模式分析表明,OSdSr4主要在水稻(OryzA SATIVA l.SSP.JAPOnICA)的茎节间和叶片中表达,干旱、高盐和低温等非生物胁迫明显抑制了OSd Sr4的表达,而脱落酸(AbSCISIC ACId,AbA)则显著诱导了它的表达;利用重叠延伸PCr方法成功克隆了OSdSr4,并将其转化进水稻中,获得了32株超表达转基因植株。分子鉴定结果表明,该基因已被整合进水稻基因组中,并在部分转基因植株中实现了超量表达。本实验为进一步开展OSdSr4基因的生物学功能研究提供了基础资料。OsDSR4 is a gene of unkown function in DUF966 gene family,and the function of DUF966 family genes have not been reported until now.In this study,the bioinformatic analysis showed that the cDNA of OsDSR4 had 2 167 bp containing an open reading frame(ORF) of 1 149 bp,and it encoded a putative protein of 372 amino acids with a highly conserved DUF966 domain.The gene expression profile analysis indicated that OsDSR4 was expressed mainly in internode and leaf blade of rice(Oryza sativa L.),and it was repressed markedly by drought,salt and cold stresses,and induced significantly by abscisic acid(ABA).OsDSR4 was cloned using overlap extension PCR,and the fusion construct containing OsDSR4 was introduced into rice(Oryza sativa L.ssp.japonica) by Agrobacterium- mediated transformation method.Thirty- two OsDSR4- overexpressing transgenic plants were obtained and identified by PCR and qRT-PCR,which was demonstated that OsDSR4 had been integrated into rice genome and was overexpressed in some positive transgenic plants.These results establish the foundation for further study of the precise function of OsDSR4.国家重点基础研究发展计划(973)前期研究专项(No.2012CB126312

Al2O3含量对PVDF/PVC/Al2O3共混中空纤维膜的影响

以聚偏氯乙烯(PVDF)、聚氯乙烯(PVC)和纳米氧化铝(Al2O3)三元共混,制备中空纤维膜,探讨Al2O3含量对膜性能的影 响。结 果表明,由于 适量Al2O3的加入,中空纤维膜膜孔结构明显改善,大孔减少,微孔增多;亲水性明显提高。Al2O3为最佳含量3%时,水通量和截留率分别达到了301L/(h·m2)和77.2%

近海生态系统碳汇过程、调控机制及增汇模式

海洋是地球上最大的碳库,发挥着全球气候变化缓冲器的作用.蓝色碳汇,简称蓝碳,即由海洋生态系统捕获的碳(主要是有机碳),是海洋储碳的重要机制之一.; 蓝碳最初认识的形式是可见的海岸带植物固碳.其实之前没有得到足够重视的、看不见的微型生物(浮游植物、细菌、古菌、病毒、原生动物)占海洋生物量90%; 以上,是蓝碳的主要贡献者.中国陆架边缘海占国土总面积的1/3,碳汇潜力巨大,亟待研发.本文以近海生态系统碳汇过程、调控机制及增汇模式为主线,论述; 了近海生态系统结构与碳循环功能特征、碳汇形成过程与机理,并结合近海碳汇在沉积记录中的地史过程演变探讨了自然过程和人类活动对碳汇的可能影响,展望了; 碳汇工程在增加近海海洋储碳能力方面的应用前景.国家重大科学研究计划项目; 国家重点研发计划项目; 国家自然科学基金项目; 国家海洋局全球变化与海气相互作用专项项

原位心脏移植（7例报告）

【目的】总结7例心脏移植经验，探讨心脏移植的近远期疗效。【方法】1998年10月至2005年4月施行7例原位心脏移植，7例均为心肌病，其中5例为扩张型，2例为限制型，手术方法采用标准法4例、双腔静脉法3例，供心平均冷缺血时间为（167．4±22．1）min，术后定期行心内膜活检，使用四联免疫抑制剂。【结果】第1例存活5d，死于低心排及主动脉内球囊反搏（IABP）的并发症，第4例存活18月，死于中一重度的急、慢性并存的排斥反应，余5例至今存活，至今存活时间分别为6年9个月、5年6个月、1年7个月、10个月、3个月。【结论】心脏移植是治疗终末期心脏病的有效手段，处理好术后并发症，密切监测和处理排斥反应，能取得良好的近远期疗效，长期生存病例需特别注意慢性排斥反应的发生

Determination of 16 Herbicides in Seawater by Solid Phase Extraction /Gas Chromatography － Mass Spectrometry

建立了同时测定海水中16种除草剂的气相色谱-质谱检测方法。样品用固相萃取仪过HLB柱浓缩、净化,洗脱液氮吹至近干,乙酸乙酯定容,用气相色谱质谱仪选择离子监测模式(SIM)进行检测,外标法定量。该方法中草净津、二甲戊乐灵的检出限为10.0 ng/L,其余14种除草剂的检出限为5.0 ng/L;草净津、二甲戊乐灵的线性范围为4.0~200μg/L,其余14种除草剂的线性范围均为2.0~100μg/L;方法加标回收率为78.3%~115.0%,相对标准偏差为4.4%~9.9%。该方法操作简单,精确度高,适用于海水中16种除草剂的定性定量检测