Clinical Trial of TB-IGRA kit by Whole Blood Culture

结核病是经结核分枝杆菌(Mycobacteriumtuberculosis，MTB)感染引起的慢性传染病，主要由空气飞沫通过呼吸道传播，近年来随着MTB耐药菌株的出现、HIV的流行、人口流动性的增加等因素呈现出逐渐蔓延的趋势。据统计，全球有近1/3的人感染了结核分枝杆菌，其中95%新发病例、99%的死亡病例集中在发展中国家。 结核杆菌进入人体后，机体对其产生的抗体并无保护作用，人体的抗结核分枝杆菌感染的免疫主要由T细胞免疫起主要作用。本研究的TB-IGRA试剂的原理是检测结核分枝杆菌感染者休内存在的特异性T细胞，在重新暴露于结核分枝杆菌抗原后释放出的IFN-γ的含量。在本实验室的前期研究工作...Tuberculosis (TB) was a chronic diseases caused by Mycobacterium tuberculosis (MTB),mainly spreaded from the air.In recent years tuberculosis showed a trend to expand by the reason of emergence of MDR-TB, prevalence of HIV, and the increased population mobility, etc. Nearly one third of people all around the World had been infected with MTB, of which 95% of new TB cases, 99% of deaths in devel...学位：理学硕士院系专业：生命科学学院生物医学科学系_生物化学与分子生物学学号：2172008115261

Usefulness of mycobacterium tuberculosis-specific interferon-γ release assay in investigating the background value of it in healthy elderly population in the region

目的:探索本地区45岁以上健康中老年人群血浆结核分支杆菌特异性γ-干扰素本底值,为Tb-IgrA定量试验广泛应用于结核病的临床诊断、疗效观察提供本底值参照依据。方法:选取100位45岁以上健康中老年人,结核分枝杆菌(MTb)γ-干扰素体外释放定量试验测定其血浆中特异性γ-干扰素含量。结果:盐城市100位健康中老年人血浆特异性γ-干扰素含量为0.1 Pg/Ml~7.1 Pg/Ml,中位数为1.1 Pg/Ml。结论:本地区健康中老年血浆中特异性γ干扰素含量存在一定的本底值,在使用Tb-IgrA定量试验方法进行结核病临床诊断、疗效观察时要充分考虑健康人群本底值带来的影响。Objective: To explore the background value of Mycobacterium Tuberculosis-specific Interferon-γ in healthy population with age over 45 years in Yancheng city and to provide reference for TB-IGRA test widely used in clinical diagnosis of TB and efficacy observation.Methods: Mycobacterium tuberculosis(MTB) interferon-γ test in vitro quantitative form was used to detect the specific interferon-α level in plasma of 100 selected healthy elderly over 45 years.Results: Plasma concentration of interferon-γ in 100 selected elderly was 0.1 pg/ml--7.1 pg/ml,with median of 1.1 pg/ml.Conclusion: There is a certain background value of MTB-specific interferon-γ in plasma of the elder over 45 years in the region,and the impact of which should be given full consideration when using the TB-IGRA quantitative test methods for clinical diagnosis of tuberculosis and efficacy observation